3 results for “Recombinant his-tagged proteins”

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Research ArticleOpen accessPhrase in the titleAn Alternative to the Traditional Western Blot For Recombinant His-tagged ProteinsJustin B. Morgenthaler, Jason R. Stephenson, Jon A. Friesen, Marjorie A. JonesA rapid, inexpensive and sensitive alternative to the traditional Western Blot is presented here. This method is very useful for detection of recombinant 6-his tagged proteins. …American Journal of Biochemistry and Biotechnology, Vol. 2, Issue 4 · 31 Dec 2006 · 6,849 accessesView PDF Cite

Research ArticleOpen accessIn the abstractInfluence of Tags on the Binding Affinity of Acyl-CoA Binding ProteinJizhong Han, Yunlong Sun, Yu Chen et al.Tagged fusion proteins are frequently employed for protein purification methods, but their effects on protein function and binding affinity are rarely studied. Here we expressed recombinant protein Acyl-CoA Binding Protein (ACBP) cloned from the full-length cDNA of Aspergillus oryzae and Saccharomyces cerevisiae. ACBP was expressed in Escherichia coli fused to a Maltose-Binding Protein (MBP) and Histidine-tag fusion. Recombinant ACBP was … …American Journal of Biochemistry and Biotechnology, Vol. 15, Issue 1 · 23 Jan 2019 · 9,102 accessesView PDF Cite

Research ArticleOpen accessIn the abstractThe Murine Charged Multivesicular Body Protein 2A, CHMP2A Interacts with the 5' and 3' Terminal Regions of Dengue Virus Complementary Minus-strand RNASuchita Chaudhry, Sathyamangalam Swaminathan, Navin KhannaDengue (DEN) viruses, of which there are four distinct serotypes (DEN-1, -2, -3 and –4), belong to the Flaviviridae family. Their ‘plus’ sense RNA genomes contain Non-Translated Regions (NTRs) at their 5’ and 3’ ends. Replication of viral genomic RNA, which takes place in close association with host cell membranes, involves the initial synthesis of the complementary minus sense RNA … …American Journal of Infectious Diseases, Vol. 2, Issue 1 · 31 Mar 2006 · 7,317 accessesView PDF Cite