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Research ArticleOpen accessIn the author namesEthanol Production by Novel Proline Accumulating Pichia kudriavzevii Mutants Strains Tolerant to High Temperature and Ethanol StressesRika Indri Astuti, Sena Alifianti, Ratu Nabila Maisyitoh et al.Accumulation of osmoprotectant molecule, proline, has been reported to induce a stress tolerance phenotype in yeast cells. In this study, two ethanologenic isolates of yeast, Pichia kudriavzevii (R and T), both capable of using both five and six sugar compounds to produce ethanol, were mutated via ethyl methanesulfonate (EMS) treatment. Prolineaccumulating mutant strains were selected by using proline-analogue (L-azetidine-2-carboxylic acid). … …OnLine Journal of Biological Sciences, Vol. 18, Issue 3 · 11 Sep 2018 · 7,838 accessesView PDFCite