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16 results for “RAPD primers”
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Research ArticleOpen accessIn the keywordsHost Plant Mediated Population Variations of Cotton Whitefly Bemisia tabaci Gennadius (Aleyrodidae: Homoptera) Characterized with Random DNA MarkersYasodha Perumal, Murugan Marimuthu, Abida Puthenpeedikal Salim, Balasubramanian PonnusamyProblem statement: Whitefly, Bemisia tabaci is an important sucking pest of field, horticultural and ornamental plants causing feeding injuries besides spreading disease by acting as a vector of Gemini viruses. The polyphagous nature of the pest makes it as a highly complex species. Approach: The influence of host plants utilized by the species on the population differences at molecular level … …American Journal of Biochemistry and Biotechnology, Vol. 5, Issue 1 · 31 Mar 2009 · 8,211 accessesView PDFCite
Research ArticleOpen accessIn the abstractIdentification of Molecular Markers Differentiating Betula papyrifera and B. pumila Populations from Northern Ontario (Canada)Nastaran Moarefi, Paul Michael, Peter Beckett, Kabwe NkongoloBetula is a polyploid and highly polymorphic genus with several species known to hybridize very readily. This high level of introgression has resulted in hybrid populations where different species are sympatric. The main objectives of the present study were to identify putative hybrids between B. papyrifera and B. pumila in the Greater Sudbury Region (Northern Ontario) and to develop ISSR … …American Journal of Environmental Sciences, Vol. 14, Issue 5 · 30 Nov 2018 · 8,057 accessesView PDFCite
Research ArticleOpen accessIn the abstractGenetic Relationship among Wild Medicinal Genotypes of Ziziphora canescens Benth. and Ziziphora tenuior L. and Detection of Genetic Variations Resulted From Tissue Culture, Salinity and pH MediaAbdulkarim Dakah, Mohamad Suleiman, Salim ZaidZiziphora tenuior L. and Ziziphora canescens Benth. are two of plants which using in folk medicine in the Kalamoon Mountains areas of Syria for cough, stomachache and dysentery. Samples of Ziziphora genotypes were collected from four different locations (Assal Al-Ward, Yabroud, Qarah and Maaraba), 20 RAPD and 12 ISSR primers were used to assess the genetic diversity of 10 genotypes. … …American Journal of Agricultural and Biological Sciences, Vol. 10, Issue 3 · 19 Oct 2015 · 7,897 accessesView PDFCite
Research ArticleOpen accessIn the abstractASSESSING THE GENETIC RELATIONSHIP OF TAIF ROSE WITH SOME ROSE GENOTYPES (ROSA SP.) BASED ON RANDOM AMPLIFIED POLYMORPHIC DNA, INTER SIMPLE SEQUENCE REPEAT AND SIMPLE SEQUENCE REPEAT MARKERSSalah El-Din El-Assal, Mohamed A. El-Awady, Adel El-Tarras, Gaber ShehabTaif-roses are a famous rose type that cultivated in Taif region and well known with their deep and intensive fragrance in the Arabian World. Despite of the great economical importance of Taif-roses for the kingdom of Saudi Arabia, their genetic origin has not been yet elucidated. The present study was mainly aimed to assess the genetic relationship between Taif-roses and … …American Journal of Biochemistry and Biotechnology, Vol. 10, Issue 1 · 29 Apr 2014 · 7,811 accessesView PDFCite
Research ArticleOpen accessIn the abstractRANDOMLY AMPLIFIED POLYMORPHIC DNA-A BRIEF REVIEWNandani Kumari, Saroj Kumar ThakurRAPD is a PCR based technique which involves the use of single arbitrary short primers (8-12 nucleotides), resulting in the amplification of many discrete DNA. The segments of DNA that are amplified are random. The technique was developed independently by two different laboratories and called as RAPD and AP-PCR (Arbitrary Primed PCR). This procedure detects nucleotide sequence polymorphisms in a … …American Journal of Animal and Veterinary Sciences, Vol. 9, Issue 1 · 2 Jan 2014 · 17,258 accessesView PDFCite
Research ArticleOpen accessIn the abstractGENOTYPIC IDENTIFICATION OF CANDIDA SPP. ISOLATED FROM ONYCHOCANDIDIASIS PATIENTS BY PHENOTYPIC METHODS, PCR AND RAPD-PCRZaidan Khlaif Imran, H. Al-GhalibiCandidal Onychomycosis (COM) is a common nail disease which plays as sources pathogenic reservoir giving a rise to repeated candidiasis infections. This study aimed to evaluate PCR assays and phenotypic tests for identification of yeasts isolated from COM patients. The study included 100 clinically suspected patients of COM attending the main hospital and clinics in Al-Dewania province in the middle … …Current Research in Medicine, Vol. 5, Issue 1 · 31 Dec 2013 · 7,263 accessesView PDFCite
Research ArticleOpen accessIn the abstractMolecular Characterization of Goats Using Random Amplified Polymorphic DnaNandani Kumari, L. B. Singh, Subodh KumarBlack Bengal goat is a very valuable goat breed in India. The present investigation was proposed to be undertaken to evaluate gene flow, phylogeographic history, genetic structure and differentiation of both the goat populations to study genetic variation prevailing among Black Bengal and Black Bengal type goat in west Bengal and Jharkhand respectively and to study genetic relationship between both … …American Journal of Animal and Veterinary Sciences, Vol. 8, Issue 1 · 20 Feb 2013 · 7,846 accessesView PDFCite
Research ArticleOpen accessIn the abstractGenetic Diversity of Some Saudi Barley (Hordeum Vulgare L.) Landraces Based on Two Types of Molecular MarkersMohamed Abdel Hamid Mohamed El-Awady, Adel Ahmed El-Sayed El-Tarras, Salah El-Din El-AssalProblem statement: Very little is known about the genetic diversity and morphological variability present in barley landrace in KSA, a country experiencing loss of biodiversity because of replacement of landraces with modern landraces. Approach: The molecular markers RAPD and ISSR were used as an efficient tools to estimate the intra-and inter-cultivar polymorphism among six barley KSA landraces collected from different … …American Journal of Applied Sciences, Vol. 9, Issue 5 · 12 Mar 2012 · 7,502 accessesView PDFCite
Research ArticleOpen accessIn the abstractDiscrimination Capacity of RAPD, ISSR and SSR Markers and of their Effectiveness in Establishing Genetic Relationship and Diversity among Egyptian and Saudi Wheat CultivarsSalah El-Din El-Assal, Ahmed GaberProblem statement: Yield crop cultivars and landraces are valuable sources of genetic variations that the knowledge and implication of these variations are critical in the plant breeding programs. our major objective of this study is investigating the discriminating capacity of RAPD, ISSR and SSR markers and of their effectiveness in establishing genetic relationship and diversity among Egyptian and Saudi wheat … …American Journal of Applied Sciences, Vol. 9, Issue 5 · 9 Mar 2012 · 7,768 accessesView PDFCite
Research ArticleOpen accessIn the abstractUsing Randomly Amplified Polymorphic DNA-Polymerase Chain Reaction of Barbodes spp. in ThailandKeeravit Petjul, Aphidech Sangdee, Bungorn Thaewnon-ngiwProblem statement: The genetic diversity of Barbodes species in Thailand is presently unavailable. The objectives of this study were to study external morphology and genetic diversity using Randomly Amplified Polymorphic DNA-Polymerase Chain Reaction (RAPD-PCR) of three Barbodes species; Barbodes altus, B. gonionotus and Barbodes sp. Approach: Two of twelve screened decanucleotide primers (OPA07 and UBC122), were selected for the genetic … …OnLine Journal of Biological Sciences, Vol. 11, Issue 1 · 6 Jul 2011 · 8,085 accessesView PDFCite