6 results for “Pyrosequencing”

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Research ArticleOpen accessEvery word in the titlePyrosequencing-An Alternative to Traditional Sanger SequencingMd Fakruddin, Abhijit ChowdhuryProblem statement: Pyrosequencing has the potential to rapidly and reliably sequence DNA taking advantages over traditional Sanger di-deoxy sequencing approach. Approach: A comprehensive review of the literature on the principles, applications, challenges and prospects of pyrosequencing was performed. Results: Pyrosequencing was a DNA sequencing technology based on the sequencing-by-synthesis principle. It employs a series of four enzymes to accurately detect … …American Journal of Biochemistry and Biotechnology, Vol. 8, Issue 1 · 2 Mar 2012 · 12,887 accessesView PDF Cite

Research ArticleOpen accessIn the abstractNerve Growth Factor (NGF) Gene Analysis in Menstrual Blood of Endometriosis: DNA Methylation and mRNA Expression LevelsOcktariyana, Nurul Hikmawati et al.Menstrual blood flow is reversed through the fallopian tubes during the menstrual process and implants outside the uterus, which is the theory of pathogenesis in endometriosis sufferers. This study aims to analyze the level of DNA methylation and mRNA expression of the Nerve Growth Factor (NGF) gene in menstrual blood in endometriosis patients and analyze the correlation. The study was … …OnLine Journal of Biological Sciences, Vol. 25, Issue 2 · 7 Aug 2025 · 4,684 accessesView PDF Cite

Research ArticleOpen accessIn the abstractThe Role of Living, Resident Bacterial Biofilm Populations in the Osseointegration of Oral ImplantsStephen Nelson, Honghua Hu et al.This study presents a human jawbone model which explains bone health, disease and quality and the osseointegration interphase, at the cell level. Curation of 454 pyrosequencing data obtained from 153 bone, serous-effusion and granulation samples taken from 40 patients during two-stage dental implant installation, revealed a 20 phyla jawbone microbiome with total genera of 387. Multivariate statistical ordination of curated … …Current Research in Dentistry, Vol. 14, Issue 1 · 16 Nov 2023 · 8,245 accessesView PDF Cite

Case ReportOpen accessIn the abstractBacterial Biofilm Persistence in Human Jawbone Following Tooth Extraction: Implications of Surgical Debridement and Resident Population-Shift for Oral ImplantsStephen Nelson, Honghua Hu et al.Intra-radicular infection theory assumes that when an infected tooth is extracted, the main source of infection is removed and any residual contaminating bacteria are killed by the immune response, then normal healing ensues. This study investigated whether bacteria and bacterial biofilms persisted in deep bone space following tooth extraction. The study was conducted on two adult male patients seeking dental … …Current Research in Dentistry, Vol. 14, Issue 1 · 31 May 2023 · 6,861 accessesView PDF Cite

Research ArticleOpen accessIn the abstractTen Bioinformatics Tools for Single Nucleotide Polymorphisms DetectionMahin Ghorbani, Hamed KarimiSingle Nucleotide Polymorphisms (SNPs) as unique genetic variations are widely used as biomarkers for drug designing and development for individualized therapy. SNP genotyping techniques such as SBE with MS detection, SBE with fluorescence intensity detection, The FP-TDI, Pyrosequencing and The TaqMan 5’ nuclease assay are low-speed, time consuming and very expensive. To overcome such disadvantages, bioinformatics tools are emerged as … …Current Research in Bioinformatics, Vol. 3, Issue 2 · 10 Apr 2015 · 17,728 accessesView PDF Cite

Research ArticleOpen accessIn the abstractChanges in the Human Skin Microbiome Over One Year’s TimeRichard W. Hyman, Farbod Babrzadeh, Curtis Palm et al.Human skin comprises a large number of distinguishable ecological niches. To describe fully the human skin microbiome, it will be necessary to identify the bacteria in each niche and to distinguish the commensal bacteria from the temporary residents. To contribute to the description of the human skin microbiome and employing a gene-based technology, we have identified the bacteria in two … …Current Research in Microbiology, Vol. 3, Issue 2 · 23 Jul 2012 · 7,043 accessesView PDF Cite