8 results for “Putative Sequence”

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Research ArticleOpen accessIn the keywordsBrevibacillus Thermoruber 9X-GLC, Bacteria Isolated from Hot Compost, Producer of a Beta-Glucosidase Resistant to Glucose InhibitionAlejandro Sosa-Peinado, María Eugenia Hidalgo-Lara, Miguel Ángel Plascencia-Espinosa et al.The thermotolerant strain Brevibacillus thermoruber 9X-GLC produces large amounts of β-D-glucosidase and cellobiohydrolase when grown on cellobiose and avicel. 9X-GLC was isolated from hot compost of sugarcane bagasse and filter mud and selected for its high enzymatic activity from a Bacilli collection of 77 isolates from soils and compost. Culture supernatants and ultrafiltered concentrates from shaken flask cultures of 9X-GLC … …American Journal of Biochemistry and Biotechnology, Vol. 13, Issue 4 · 20 Oct 2017 · 8,153 accessesView PDF Cite

Research ArticleOpen accessIn the abstractStructural and Substrate Interaction Properties of Alkaline Phosphatase from Paenibacillus sp. PSB04: In-Silico AnalysisHerman Umbau Lindang, Cahyo BudimanA Phosphate-Solubilising Bacterium (PSB) of Paenibacillus sp. PSB04 was previously isolated from the Sabah tropical rainforest in Malaysia. Interestingly, the genome sequence of the PSB04 strain harbored an Alkaline Phosphatase (AP) (EC 3.1.3.1) gene and was hypothesized to have unique structural characteristics. Therefore, this study aims to determine the AP three-Dimensional (3D) model and catalytic mechanism from Paenibacillus sp. PSB04 … …OnLine Journal of Biological Sciences, Vol. 22, Issue 3 · 16 Jul 2022 · 5,910 accessesView PDF Cite

Research ArticleOpen accessIn the abstractIdentification and Characterization of Diacylglycerol Acyltransferase in Oleaginous Yeast Rhodosporidium toruloidesZhen Wang, Huaiyuan Zhang, Lina Zhao, Yuanda SongDiacylglycerol acyltransferase (DGAT), which catalyzes TAG formation from DAG and acyl-CoA, has been considered to play a vital role in TAG accumulation in oleaginous microorganisms. The genome of oleaginous yeast Rhodosporidium toruloides contains two putative DGAT genes, RtDGATa and RtDGATb, which shared little conserved amino acid coding sequence with each other. Phylogeny tree analysis showed that RtDGATa belonged to DGAT1 … …American Journal of Biochemistry and Biotechnology, Vol. 12, Issue 4 · 17 Oct 2016 · 7,387 accessesView PDF Cite

Research ArticleOpen accessIn the abstractIn Silico Analysis of Cellulose Synthase Gene (NcCesA1) in Developing Xylem Tissues of Neolamarckia CadambaTiong Shing Yiing, Ho Wei Seng, Pang Shek Ling, Ismail JusohThis study reported the isolation and in silico characterization of full-length cellulose synthase (CesA) cDNA from Neolamarckia cadamba, an important tropical plantation tree species. CesA is a member of processive glycosyltransferases that involved in cellulose biosynthesis of plants. CesA acts as a central catalyst in the generation of plant cell wall biomass or cellulose. It also plays an important role … …Current Research in Bioinformatics, Vol. 3, Issue 2 · 13 Jan 2015 · 7,990 accessesView PDF Cite

Research ArticleOpen accessIn the abstractDetecting Patterns in 5’ Untranslated Regions of Genes Involved in the Regulation of Blood Pressure and Oxidative StressMaria Sanchez-Aguilar, Luz Ibarra-Lara, Beatriz Zamora-Lopez et al.Hypertension is a chronic physical condition with multifactorial causes. Although genetic factors have been associated to this pathological condition, characterization of the sequence patterns in the regulatory sites, for example, in 3’ or 5’ Untranslated Regions (UTR), remains to be explored. In particular, the 5’ UTR of genes associated with the regulation of blood pressure and oxidative stress are analyzed … …Current Research in Medicine, Vol. 4, Issue 2 · 27 Nov 2013 · 7,283 accessesView PDF Cite

Research ArticleOpen accessIn the abstractRat Cardiomyocytes Express a Classical Epithelial Beta-DefensinAnnika Linde, Gerald H. Lushington, Frank Blecha, Tonatiuh MelgarejoBeta-defensins (BDs) are classical epithelial antimicrobial peptides of immediate importance in innate host defense. Since recent studies have suggested that certain BDs are also expressed in non-traditional tissues, including whole heart homogenate, and because effector molecules of innate immunity and inflammation can influence the development of certain cardiovascular disease processes, we hypothesized that BDs are produced by cardiomyocytes as a … …American Journal of Animal and Veterinary Sciences, Vol. 3, Issue 1 · 31 Mar 2008 · 6,994 accessesView PDF Cite

Research ArticleOpen accessIn the abstractPrediction of a Putative Functional Region in the Human Bax Protein by Computational AnalysisMaría Sánchez-Aguilar, Laurence A. Marchat, Absalom ZamoranoStructural domains are relevant elements for interactions between Bcl-2 family members to regulate apoptosis and cell survival. Although BH1-BH4 domains can be identified by their sequence and selective occurrence in Bcl-2-family members, structural regions have not been entirely determined yet. The functional residues of Bax, the most representative pro-apoptotic protein of the human Bcl-2 family, remain almost unknown. Here, we … …American Journal of Infectious Diseases, Vol. 3, Issue 2 · 30 Jun 2007 · 7,230 accessesView PDF Cite

Research ArticleOpen accessIn the abstractMolecular Characterization of Soybean Mosaic Virus NIa Protein and its Processing Event in Bacterial ExpressionBong K. Choi, Jae S. Park, Hye J. Ahn et al.Soybean mosaic virus (SMV)-CN18 is an Rsv resistance-breaking (RB) isolate to overcome soybean resistance genes Rsv1, Rsv3 and Rsv4. The aim of this study was to characterize nuclear inclusion protein a (NIa protein) of RB isolate at the molecular level and demonstrate its processing into genome-linked protein (VPg) and NIa-Pro domains in Esherichia coli containing a bacterial expression pET vector … …American Journal of Biochemistry and Biotechnology, Vol. 2, Issue 4 · 31 Dec 2006 · 6,902 accessesView PDF Cite