Review ArticleOpen accessPhrase in the titleAssessing Protein PurityShiva Siddappa, Liang-Yin Ke, Chu-Huang Chen, Gopal Kedihithlu MaratheDetermination of protein purity is crucial, as proteins are frequently used as therapeutic agents and reagents in many biochemical reactions. Additionally, purified protein is necessary for biophysical and structural studies. Assessing protein purity involves examining for impurities or demonstrating their absence. In our experience, a satisfactory answer is seldom obtained when biochemistry students are asked a common question on how … …American Journal of Biochemistry and Biotechnology, Vol. 21, Issue 4 · 9 Feb 2026 · 1,901 accessesView PDFCite
Research ArticleOpen accessIn the abstractThe Deproteinization, Antioxidant Acticities and Inhibitory Effect on α-Amylase of Polysaccharides from Corn SilkYaping Li, Songbiao Shi, Xiudong Yang, Hongli ZhouAn approach of deproteinization of polysaccharides from corn silk (CSPs) was established to improve the purity and activities of CSPs by investigating Sevag method, trichloroacetic acid (TCA) method and HCl method. Deproteinization rate, polysaccharide retention rate, antioxidant activities and inhibition α-amylase effects were used as indicative mark to select suitable deproteinization method of CSPs. The crude CSPs was extracted by … …American Journal of Biochemistry and Biotechnology, Vol. 15, Issue 2 · 23 May 2019 · 9,294 accessesView PDFCite
Research ArticleOpen accessIn the abstractInfluence of Tags on the Binding Affinity of Acyl-CoA Binding ProteinJizhong Han, Yunlong Sun, Yu Chen et al.Tagged fusion proteins are frequently employed for protein purification methods, but their effects on protein function and binding affinity are rarely studied. Here we expressed recombinant protein Acyl-CoA Binding Protein (ACBP) cloned from the full-length cDNA of Aspergillus oryzae and Saccharomyces cerevisiae. ACBP was expressed in Escherichia coli fused to a Maltose-Binding Protein (MBP) and Histidine-tag fusion. Recombinant ACBP was … …American Journal of Biochemistry and Biotechnology, Vol. 15, Issue 1 · 23 Jan 2019 · 9,097 accessesView PDFCite