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10 results for “Lipolytic”
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Research ArticleOpen accessEvery word in the titleThe Enzymatic Role of Lipid Metabolism in Yarrowia lipolytica Grown on GlycerolRuixue Wang, Feifei Xin, Yufei Chang et al.More attention has been paid to the production of commercial fatty acids by microbial fermentation. The basic biochemistry of lipid accumulation in oleaginous microorganisms under nitrogen limitation has been extensively studied. As an oleaginous microorganism, Yarrowia lipolytica can produce fatty acid-derived biofuels and biochemicals. However, the response of the enzymes in Y. lipolytica to glycerol remains unclear. Therefore, we aimed … …American Journal of Biochemistry and Biotechnology, Vol. 19, Issue 2 · 17 Apr 2023 · 6,309 accessesView PDFCite
Research ArticleOpen accessEvery word in the titleTranscriptomic Analysis of the Erythritol High-Yielding Mutant Strain Yarrowia lipolytica Y44Xiaojie Ren, Heng Ban, Chao Huang et al.RNA-seq technique was used to analyze the transcriptomics of Yarrowia lipolytica control strain Po1g, an erythritol-producing natural isolate Yarrowia lipolytica Y 22 and an erythritol high-yielding mutant strain Y44 derived from Y 22. Functional annotation of genes and classification of metabolic pathways were carried out to identify Differentially Expressed Genes (DEGs). These DEGs were classified into related metabolic pathways to … …American Journal of Biochemistry and Biotechnology, Vol. 17, Issue 4 · 20 Dec 2021 · 6,115 accessesView PDFCite
Research ArticleOpen accessEvery word in the titleKnock Down of Erythrulose Kinase (eyk1) Leads to the Enhancement of Erythritol Production in Yarrowia lipolytica Mutant Strain YE4-2Xinhe Zhao, Tianlong Huang, Yaxin Zhang et al.Erythritol is a nearly zero-calorie sweetener, which is in great demand in food and health care industry. Our previous work obtained a Yarrowia lipolytica mutant strain YE4-2 with high erythritol yield. However, its conversion rate of glucose needs further improvement. Erythrulose kinase (eyk1) participates in the early stages of erythritol catabolism; the existence of eyk1 prevents the accumulation of erythritol. … …American Journal of Biochemistry and Biotechnology, Vol. 16, Issue 3 · 24 Jul 2020 · 6,750 accessesView PDFCite
Research ArticleOpen accessEvery word in the titleCocoa-Butter-Equivalent Production from Yarrowia lipolytica by Optimization of Fermentation TechnologyLina Zhao, Bo Li, Dan Xiong et al.In order to obtain the optimal control point for single-cell lipid production with fatty acid profile similar to cocoa butter, this study explored the effects of carbon source, nitrogen source, temperature, sterculic acid methyl ester, cobalt on cell growth and lipid accumulation of Yarrowia lipolytica. Glycerol and ammonium tartrate-yeast extract were chosen as carbon source and nitrogen source for production … …American Journal of Biochemistry and Biotechnology, Vol. 12, Issue 4 · 29 Dec 2016 · 9,285 accessesView PDFCite
Research ArticleOpen accessEvery word in the titleConversion of Mutton Fat to Cocoa Butter Equivalent by Increasing the Unsaturated Fatty Acids at the Sn-2 Position of Triacylglycerol Through Fermentation by Yarrowia LipolyticaDan Xiong, Huaiyuan Zhang, Yifang Xie et al.Mutton fat has a similar fatty acid profile with Cocoa Butter (CB), except that its degree of unsaturation of Triacylglycerol (TAG) at the Sn-2 position is considerably lower than CB and maybe increased by Sn-2 specific lipase to produce Cocoa Butter Equivalent (CBE), a healthy functional lipid. However, there is no commercially available Sn-2 specific lipase that can be used … …American Journal of Biochemistry and Biotechnology, Vol. 11, Issue 2 · 4 Mar 2015 · 8,698 accessesView PDFCite
Research ArticleOpen accessIn the keywordsMicroflora Contribution to Cellulase and Digestion Enzyme in Mud Crab Digestive TractSiti Aslamyah, Yushinta Fujaya, Nita RukminasariMicroflora in the digestive tract of aquatic animals is thought to be not only the microbes that contribute to the production of cellulase enzymes but also contribute to other digestive enzymes, such as protease, amylase and lipase. This study aims to examine the microflora contribution to the mud crab’s digestive tract. Scylla sp. were maintained in the crab box individually … …OnLine Journal of Biological Sciences, Vol. 21, Issue 1 · 26 Mar 2021 · 6,453 accessesView PDFCite
Research ArticleOpen accessIn the keywordsBiochemical Studies of Lipase from Germinating Oil Seeds (Glycine max)P. P. Gadge, S. D. Madhikar, J. N. Yewle et al.Problem statement: Lipase is one of the important enzymes in food, pharmaceutical, detergent and biofuels industries. Search for the lipase with distinct features, possibly from germinating seeds, is of interest for industrial applications. Approach: The lipase produced by soybean oil seeds was partially purified and characterized in terms of the optimal pH and temperature for activity as well as substrate … …American Journal of Biochemistry and Biotechnology, Vol. 7, Issue 3 · 19 Jan 2012 · 9,703 accessesView PDFCite
Research ArticleOpen accessIn the keywordsThe Body Calming and Body Vitalizing Effects of Vernonia Amygdalina Leaf Extract and Leaf Powder and their MechanismsUtoh-Nedosa Uchechukwu AnastasiaProblem statement: Vernonia amygdalina (V. amygdalina) leaf soup is regarded by the indigenous Igbo people of South Eastern Nigeria as their best leafy vegetable soup. Despite the bitterness of the bitter leaf soup, the native eaters of the soup feel very content when they eat pounded carbohydrate foods with it. Oral V. amygdalina leaf extract was taken methodically in this … …American Journal of Agricultural and Biological Sciences, Vol. 6, Issue 3 · 20 Sep 2011 · 8,163 accessesView PDFCite
Research ArticleOpen accessIn the abstractInvestigation of the Contribution of Late Embryogenesis Abundant (LEA) K Peptide in Enhancing the Expression of Lipase from Sphingobacterium sp.: In vitro and in Silico StudiesIbrahim Muhammad et al.Sphingobacterium sp. AB3 lipase is a psychrophilic enzyme with optimal lipolytic activity at pH 7 and 15°C. The cold-adapted properties of the lipase render it suitable for various low-temperature industrial applications such as biodiesel production, detergent formulation, and non-thermal food processing. Recent studies have shown that co-expression of LEA K peptide with AB3 lipase resulted in enhanced protein expression in … …American Journal of Biochemistry and Biotechnology, Vol. 20, Issue 4 · 21 Nov 2024 · 4,569 accessesView PDFCite
Research ArticleOpen accessIn the abstractProduction, Extraction and Characterization of Lipases from the Antarctic Yeast Guehomyces pullulansLuis Lascano-Demera, Patricia Portero Barahona, Enrique Javier Carvajal BarrigaThe production of extracellular lipases from the Antarctic yeast Guehomyces pullulans is induced using an olive oil medium as an inductor substrate and a first characterization of its enzyme, using the protein extract obtained from the medium, is described. For this, the effect of pH and temperature on the lipase activity are evaluated and the enzyme kinetic for the lipase … …American Journal of Biochemistry and Biotechnology, Vol. 15, Issue 2 · 11 Apr 2019 · 10,020 accessesView PDFCite