3 results for “Indirect Immunofluorescence”

Research ArticleOpen accessIn the keywordsSerological and Molecular Investigation of Cutaneous Leishmaniasis in Healthy Individuals from an American Cutaneous Leishmaniasis-Endemic RegionRejane Cristina Ribas-Silva, Taisa Rocha Navasconi, Laís de Souza Braga et al.American Cutaneous Leishmaniasis (ACL) is a major, clinically relevant zoonosis in Brazil. The disease spectrum includes single, localized, cutaneous ulcers, diffuse cutaneous leishmaniasis and mucosal disease. A subclinical form of the disease has also been described in individuals living in ACL-endemic regions. The goal of this study was to employ immunological and molecular diagnostic methods to evaluate the presence of … …American Journal of Infectious Diseases, Vol. 11, Issue 1 · 23 Jun 2015 · 7,324 accessesView PDF Cite

Research ArticleOpen accessIn the abstractComparison of enzyme Immunoassay with Immunofluorescence Assay for the Diagnosis of Mycoplasma pneumoniae Respiratory Tract Infections in ChildrenOsama Mohammed Saed Abdul-WahabMycoplasma pneumoniae is a frequent cause of acute respiratory infections in both children and adults. Currently, diagnosis of M. pneumoniae infection will based principally on serology and the detection of IgM can provide an early and sensitive diagnosis in children. A variety of commercial immunodiagnostic assays, such as Indirect Enzyme-Linked Immunosorbent (ELISA) assay and Indirect Immunofluorescence (IFA), are now available … …American Journal of Immunology, Vol. 9, Issue 2 · 21 Jun 2013 · 7,412 accessesView PDF Cite

Research ArticleOpen accessIn the abstractImmunohistochemistry Assay to Detect Turkey Coronavirus (TCoV) from Experimentally Infected PoultsThais Larissa L Castanheira, Heitor F. Ferrari, Maria Cecília R. Luvizotto, Tereza C. CardosoThe objective of this study was to develop a direct immunohistochemical assay to detect TCoV antigens in formalin-fixed paraffin-embedded sections prepared from experimentally infected poults. The sections of ileo, ileo-cecal junction and ceca regions from intestine were prepared and submitted to two different primary antibodies, first the non-biotin labeled polyclonal antibody for the indirect method, and second the biotin-labeled polyclonal … …American Journal of Immunology, Vol. 3, Issue 2 · 31 Dec 2007 · 6,900 accessesView PDF Cite