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9 articles for “ISSR Analysis”
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Research ArticleOpen accessIn the keywordsDNA Yields and PCR Amplification Success Using Degraded Animal CorpsesJimmy Ilunga, Paul Michael, Grace Daniel et al.A recurring problem with ancient specimens from wildlife animals is that their preserved tissues contain small amounts of DNA in a degraded state. The specific objectives of the present study were: (1) To determine DNA yields from different animal tissues; (2) to compare traditional (manual) DNA extraction protocols with commercial procedures and (3) to assess the success of PCR amplification … …American Journal of Biochemistry and Biotechnology, Vol. 16, Issue 1 · 12 Feb 2020 · 7,479 accessesView PDFCite
Research ArticleOpen accessIn the keywordsASSESSING THE GENETIC RELATIONSHIP OF TAIF ROSE WITH SOME ROSE GENOTYPES (ROSA SP.) BASED ON RANDOM AMPLIFIED POLYMORPHIC DNA, INTER SIMPLE SEQUENCE REPEAT AND SIMPLE SEQUENCE REPEAT MARKERSSalah El-Din El-Assal, Mohamed A. El-Awady, Adel El-Tarras, Gaber ShehabTaif-roses are a famous rose type that cultivated in Taif region and well known with their deep and intensive fragrance in the Arabian World. Despite of the great economical importance of Taif-roses for the kingdom of Saudi Arabia, their genetic origin has not been yet elucidated. The present study was mainly aimed to assess the genetic relationship between Taif-roses and … …American Journal of Biochemistry and Biotechnology, Vol. 10, Issue 1 · 29 Apr 2014 · 7,811 accessesView PDFCite
Research ArticleOpen accessIn the abstractGenetic State of Pulsatilla Patens (L.) Mill. Coenopopulations Based on DNA LabelingRustem Saltanovich Beishov, Gulnar Sultangazina, Indira Saltanovna Beishova et al.In the article, the results of studying the gene pools of the Kazakhstan coenopopulations of a rare plant species Pulsatilla patens (L.) Mill. are shown. The biomaterial taken from five coenopopulations located in Northern Kazakhstan has been used as samples. To identify the multilocus DNA polymorphism, the authors have used ISSR (Inter Simple Sequence Repeats, or intermicrosatellite analysis) labeling. The … …OnLine Journal of Biological Sciences, Vol. 21, Issue 1 · 2 Apr 2021 · 5,944 accessesView PDFCite
Research ArticleOpen accessIn the abstractComparison of Molecular Markers ISSR, SSR and RFLP to Study the Genetic Variation of Strawberry CultivarsGanies Riza Aristya, Rina Kasiamdari, Muhammad Fauzi Arif et al.Strawberry is grown in Indonesia because it has potential high economic value. The purpose of this study was to investigate relationship of phenetic lineage and genetic variations in 12 strawberry cultivars using three different molecular marker methods, including ISSR, SSR and RFLP. Five primers of ISSR markers were used and they generated 53 bands with 44 polymorphic DNA bands. Percentage … …American Journal of Agricultural and Biological Sciences, Vol. 14, Issue 1 · 19 Mar 2019 · 9,182 accessesView PDFCite
Research ArticleOpen accessIn the abstractCOMPARATIVE ANALYSIS OF INTER SIMPLE SEQUENCE REPEATS AND SIMPLE SEQUENCE REPEATS MARKERS: GENETIC ANALYSIS OF DESCHAMPSIA CESPITOSA POPULATIONS GROWING IN METAL CONTAMINATED REGIONS IN CANADAK.K. Nkongolo, S. Gervais, P. Michael, Y. ZhouComparative studies conducted on the genetic variation of metal-tolerant populations and their non-metal-tolerant counterparts have been performed on numerous species using isozyme markers. Analysis of genetic differences among plant populations growing in heavy metal-contaminated and uncontaminated regions are limited. The main objectives of the present study were to compare ISSR and microsatellite markers in assessing genetic variation in D. cespitosa … …American Journal of Biochemistry and Biotechnology, Vol. 10, Issue 1 · 8 Apr 2014 · 7,286 accessesView PDFCite
Research ArticleOpen accessIn the abstractDiscrimination Capacity of RAPD, ISSR and SSR Markers and of their Effectiveness in Establishing Genetic Relationship and Diversity among Egyptian and Saudi Wheat CultivarsSalah El-Din El-Assal, Ahmed GaberProblem statement: Yield crop cultivars and landraces are valuable sources of genetic variations that the knowledge and implication of these variations are critical in the plant breeding programs. our major objective of this study is investigating the discriminating capacity of RAPD, ISSR and SSR markers and of their effectiveness in establishing genetic relationship and diversity among Egyptian and Saudi wheat … …American Journal of Applied Sciences, Vol. 9, Issue 5 · 9 Mar 2012 · 7,768 accessesView PDFCite
Research ArticleOpen accessIn the abstractMolecular Characterization of Opium Poppy (Papaver somniferum) GermplasmHari Shanker Acharya, Vimal SharmaProblem statement: The medicinal value of opium poppy (2n = 22, Papaver somniferum L.) is due to presence of more than two dozen alkaloids. The genetic origin and diversity of cultivars used in the breeding collection is not fully known. To evaluate the genetic diversity of the 24 germplasm currently cultivated in the state to provide genetic information about heterogeneity, … …American Journal of Infectious Diseases, Vol. 5, Issue 2 · 30 Jun 2009 · 8,096 accessesView PDFCite
Research ArticleOpen accessIn the abstractMolecular Characterization of Tospovirus Transmitting Thrips Populations from IndiaRang Lal Meena, T. Ramasubramanian, S. Venkatesan, S. MohankumarThe presence of Tospovirus in thrips vector, Scirtothrips dorsalis has been detected for the first time in India using RT- PCR analysis. Similarly RT-PCR analysis with GBNV infected tomato leaves also resulted in the amplification of cDNA corresponding to the N gene (approximately 830 bp) of Coimbatore isolate. Dendrogram constructed on the basis of RAPD similarity matrix revealed that S. … …American Journal of Biochemistry and Biotechnology, Vol. 1, Issue 3 · 30 Sep 2005 · 6,733 accessesView PDFCite
Research ArticleOpen accessAll words, across the recordHEAVY METAL ANALYSIS IN RED OAK (QUERCUS RUBRA) POPULATIONS FROM A MINING REGION IN NORTHERN ONTARIO (CANADA): EFFECT OF SOIL LIMING AND ANALYSIS OF GENETIC VARIATIONAnh Tran, K. K. Nkongolo, M. Mehes-Smith et al.Understanding the dynamic of metals in soil and plants and population diversity in Northern Ontario is essential in determining progress toward ecosystem sustainability in reclaimed sites. The objectives of the present study were to assess the levels of metal content in soils and their accumulation in red oak plants from limed and unlimed sites. Genetic variation in red oak populations … …American Journal of Environmental Sciences, Vol. 10, Issue 4 · 25 Jun 2014 · 7,732 accessesView PDFCite