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16 articles for “ISSR”
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Research ArticleOpen accessEvery word in the titleDiscrimination Capacity of RAPD, ISSR and SSR Markers and of their Effectiveness in Establishing Genetic Relationship and Diversity among Egyptian and Saudi Wheat CultivarsSalah El-Din El-Assal, Ahmed GaberProblem statement: Yield crop cultivars and landraces are valuable sources of genetic variations that the knowledge and implication of these variations are critical in the plant breeding programs. our major objective of this study is investigating the discriminating capacity of RAPD, ISSR and SSR markers and of their effectiveness in establishing genetic relationship and diversity among Egyptian and Saudi wheat … …American Journal of Applied Sciences, Vol. 9, Issue 5 · 9 Mar 2012 · 7,768 accessesView PDFCite
Research ArticleOpen accessEvery word in the titleGenetic Diversity of Common Wild Wheat Species in Armenia Using ISSR MarkersManvel Badalyan, Tatevik Aloyan, Varya Dilanyan et al.Armenia is one of the primary genealogical centers of numerous crops, the wild relatives of many of which are still found in Armenia today. Population limits of three out of 4 species of wild relatives of endemic wheat in Armenia (T. araraticum Jakubz, T. urartu Tumanian ex Gandilyan, T. boeoticum Boiss.) have been reduced in recent decades as a result … …OnLine Journal of Biological Sciences, Vol. 23, Issue 3 · 15 Aug 2023 · 5,217 accessesView PDFCite
Research ArticleOpen accessEvery word in the titleComparison of Molecular Markers ISSR, SSR and RFLP to Study the Genetic Variation of Strawberry CultivarsGanies Riza Aristya, Rina Kasiamdari, Muhammad Fauzi Arif et al.Strawberry is grown in Indonesia because it has potential high economic value. The purpose of this study was to investigate relationship of phenetic lineage and genetic variations in 12 strawberry cultivars using three different molecular marker methods, including ISSR, SSR and RFLP. Five primers of ISSR markers were used and they generated 53 bands with 44 polymorphic DNA bands. Percentage … …American Journal of Agricultural and Biological Sciences, Vol. 14, Issue 1 · 19 Mar 2019 · 9,182 accessesView PDFCite
Research ArticleOpen accessIn the keywordsGenetic State of Pulsatilla Patens (L.) Mill. Coenopopulations Based on DNA LabelingRustem Saltanovich Beishov, Gulnar Sultangazina, Indira Saltanovna Beishova et al.In the article, the results of studying the gene pools of the Kazakhstan coenopopulations of a rare plant species Pulsatilla patens (L.) Mill. are shown. The biomaterial taken from five coenopopulations located in Northern Kazakhstan has been used as samples. To identify the multilocus DNA polymorphism, the authors have used ISSR (Inter Simple Sequence Repeats, or intermicrosatellite analysis) labeling. The … …OnLine Journal of Biological Sciences, Vol. 21, Issue 1 · 2 Apr 2021 · 5,944 accessesView PDFCite
Research ArticleOpen accessIn the keywordsDNA Yields and PCR Amplification Success Using Degraded Animal CorpsesJimmy Ilunga, Paul Michael, Grace Daniel et al.A recurring problem with ancient specimens from wildlife animals is that their preserved tissues contain small amounts of DNA in a degraded state. The specific objectives of the present study were: (1) To determine DNA yields from different animal tissues; (2) to compare traditional (manual) DNA extraction protocols with commercial procedures and (3) to assess the success of PCR amplification … …American Journal of Biochemistry and Biotechnology, Vol. 16, Issue 1 · 12 Feb 2020 · 7,479 accessesView PDFCite
Research ArticleOpen accessIn the keywordsIdentification of Molecular Markers Differentiating Betula papyrifera and B. pumila Populations from Northern Ontario (Canada)Nastaran Moarefi, Paul Michael, Peter Beckett, Kabwe NkongoloBetula is a polyploid and highly polymorphic genus with several species known to hybridize very readily. This high level of introgression has resulted in hybrid populations where different species are sympatric. The main objectives of the present study were to identify putative hybrids between B. papyrifera and B. pumila in the Greater Sudbury Region (Northern Ontario) and to develop ISSR … …American Journal of Environmental Sciences, Vol. 14, Issue 5 · 30 Nov 2018 · 8,057 accessesView PDFCite
Research ArticleOpen accessIn the keywordsReassessment of Molecular Variation in Isolated Populations of Deschampsia cespitosa from Metal Contaminated Regions in Northern Ontario (Canada) after 17 Years of Potential Genetic RecombinationSabrina Rainville, Peter Beckett, Kabwe NkongoloThe effects of ore extraction and processing procedures in the Greater Sudbury and Cobalt regions have been long-lasting. The objective of this study is to determine the current level of genetic variation in Deschampsia cespitosa populations from metal contaminated and uncontaminated sites in samples collected in Northern Ontario in 2016 after 17 years of potential genetic recombination since the last … …American Journal of Environmental Sciences, Vol. 13, Issue 4 · 13 Jul 2017 · 7,044 accessesView PDFCite
Research ArticleOpen accessIn the keywordsDevelopment, Polymorphism and Cross-Species Transferability of Genomic SSR Markers in Duabanga Moluccana, an Indigenous Tree Species from SarawakKit-Siong Liew, Wei-Seng Ho, Shek-Ling Pang, Julaihi AbdullahIn this study, we used ISSR-suppression methods to develop a set of SSR markers for Duabanga moluccana. It is an indigenous fast growing tropical tree species. A total of 44 SSR regions were identified and specific primer pairs were designed. The SSR motifs contained perfect compound with 24 (54.5%) occurrences, followed by the imperfect compounds with 8 (18.2%), simple perfect … …OnLine Journal of Biological Sciences, Vol. 16, Issue 1 · 26 Jan 2016 · 7,784 accessesView PDFCite
Research ArticleOpen accessIn the keywordsGenetic Relationship among Wild Medicinal Genotypes of Ziziphora canescens Benth. and Ziziphora tenuior L. and Detection of Genetic Variations Resulted From Tissue Culture, Salinity and pH MediaAbdulkarim Dakah, Mohamad Suleiman, Salim ZaidZiziphora tenuior L. and Ziziphora canescens Benth. are two of plants which using in folk medicine in the Kalamoon Mountains areas of Syria for cough, stomachache and dysentery. Samples of Ziziphora genotypes were collected from four different locations (Assal Al-Ward, Yabroud, Qarah and Maaraba), 20 RAPD and 12 ISSR primers were used to assess the genetic diversity of 10 genotypes. … …American Journal of Agricultural and Biological Sciences, Vol. 10, Issue 3 · 19 Oct 2015 · 7,897 accessesView PDFCite
Research ArticleOpen accessIn the keywordsHEAVY METAL ANALYSIS IN RED OAK (QUERCUS RUBRA) POPULATIONS FROM A MINING REGION IN NORTHERN ONTARIO (CANADA): EFFECT OF SOIL LIMING AND ANALYSIS OF GENETIC VARIATIONAnh Tran, K. K. Nkongolo, M. Mehes-Smith et al.Understanding the dynamic of metals in soil and plants and population diversity in Northern Ontario is essential in determining progress toward ecosystem sustainability in reclaimed sites. The objectives of the present study were to assess the levels of metal content in soils and their accumulation in red oak plants from limed and unlimed sites. Genetic variation in red oak populations … …American Journal of Environmental Sciences, Vol. 10, Issue 4 · 25 Jun 2014 · 7,732 accessesView PDFCite