4 results for “Hideaki Yamashiro”

Research ArticleOpen accessIn the author namesOxidation of Exogenous Lactate by Lactate Dehydrogenase C in the Midpiece of Rat Epididymal Sperm is Essential for Motility and Oxidative ActivityHideaki Yamashiro, Masaaki Toyomizu, Akane Kadowaki et al.Problem statement: To identify the metabolic reaction-glycolysis or oxidative phosphorylation that is mainly involved in the production of energy required for rat sperm mobilization. Approach: Epididymal sperm were collected from Wistar rats and extended in lactate-containing or lactate-free raffinose-modified Krebs-Ringer Bicarbonate solution (mKRB)-egg yolk medium supplemented with 0, 1, 2, or 3 mM 2-Deoxy-D-Glucose (2 DG) and 1, 2, or … …American Journal of Applied Sciences, Vol. 6, Issue 10 · 31 Oct 2009 · 6,901 accessesView PDF Cite

Research ArticleOpen accessIn the author namesInfluence of the Prostatic Fluid from the First and Third Fractions of the Ejaculates on the Cryosurvival of Poodle Dog SpermHideaki Yamashiro, Kaori Narita, Satoshi Sugimura et al.Problem statement: Two experiments were conducted to determine the effect of the prostatic fluid from the first and third fractions of the ejaculates on the freezability of poodle dog sperm. Approach: The ejaculates from 2 trained poodle dogs were collected. Effect of prostatic fluid from the first and third fractions of ejaculates on cryosurvival of dog sperm was examined. SDS-PAGE … …American Journal of Animal and Veterinary Sciences, Vol. 4, Issue 1 · 31 Mar 2009 · 7,831 accessesView PDF Cite

Research ArticleOpen accessIn the author namesNoninvasive Measurement of Fecal Progesterone Concentration in Toy Poodles by Time Resolved Fluoroimmunoassay (TR-FIA)Satoshi Sugimura, Kaori Narita, Hideaki Yamashiro et al.Progesterone is an important reproductive hormone and measurement of its level by repeated blood samplings is beneficial to monitoring of estrus cycle. However, since toy poodles have a small body size and thin-walled blood vessels, repeated blood samplings cause stress and affect their preparation for mating or artificial insemination (AI). Therefore, a noninvasive method for monitoring progesterone concentration should be … …American Journal of Animal and Veterinary Sciences, Vol. 3, Issue 1 · 31 Mar 2008 · 6,564 accessesView PDF Cite