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35 articles for “HPLC method”
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Research ArticleOpen accessPhrase in the titleA HPLC Method for Detection of 17 Characteristic Components in Tea ExtractYing Liu, Dan Wu, Ping Tang, Xingqian YeA HPLC method has been developed for the determination of tea for seven catechins ((+)-catechin, (−)-epicatechin, (−)-epigallocatechin, (−)-epigallocatechin gallate, (−)-epicatechin gallate, (−)-gallocatechins and (−)-gallocatechin gallate), two flavonols (quercetin, kaempferol), seven organic acids (Gallic acid, ferulic acid, protocatechuic acid, p-hydroxybenzoic acid，p-coumaric acid, erucic acid，vanillic acid) and caffeine. Using HPLC with gradient elution and photodiode array detection, seventeen compounds were separated and … …American Journal of Biochemistry and Biotechnology, Vol. 18, Issue 1 · 2 Feb 2022 · 7,028 accessesView PDFCite
Research ArticleOpen accessEvery word in the titleBioanalytical Method Validation and Quantification of Flupirtine in Canine Plasma by HPLC with Spectrofluorimetric DetectionVirginia De Vito, Alessandro Saba, Helen Owen, Mario GiorgiFlupirtine (FLU) is a non-opioid analgesic drug belongs to the class of N-Methyl-D-Aspartate (NMDA) antagonist without antipyretic or antiphlogistic properties. No analytical method to detect FLU in canine plasma samples through a fluorimetric detector has been published to date. The analytical method described in this work provides a selective and accurate quantification of FLU. The mobile phase consisted of ACN:AcONH4 … …American Journal of Animal and Veterinary Sciences, Vol. 10, Issue 2 · 22 Jun 2015 · 7,982 accessesView PDFCite
Research ArticleOpen accessIn the keywordsEffect of Verapamil on Serum Level of Salinomycin in Diabetic RatsHossein Najafzadeh, Said Reza Fatemi, Amir AshnaProblem statement: The aim of present study was evaluation function of P-glycoprotein with or without verapamil in normal and diabetic rats; which the function of P-glycoprotein was indirectly evaluated by detection of serum slainomycin concentration with HPLC method. Approach: This study was carried in 4 groups of rats including normal rats which received salinomycin and verapamil together salinomycin; and diabetic … …American Journal of Applied Sciences, Vol. 8, Issue 9 · 1 Aug 2011 · 6,823 accessesView PDFCite
Research ArticleOpen accessIn the abstractUnveiling the Potential of Dombeya buettneri K.Schum As a Drug Agent: Molecular Docking of Compounds With Enzymes Linked to Diabetes and Neurodegenerative DiseaseEsther Emem Nwanna, Samuel Chima Ugbaja et al.This study investigated the mechanisms of Dombeya buettneri (snowball leafy) extracts on diabetes, hypertension, and neurodegenerative diseases. It compared extracts obtained using steep water (a by-product of corn wet milling) and aqueous water. The extracts were analyzed for total phenol, total flavonoid content, antioxidant activities such as FRAP, ABTS, NO, DPPH, iron-induced lipid peroxidation assay, degradation of deoxyribose (Fenton’s reaction), … …OnLine Journal of Biological Sciences, Vol. 25, Issue 4 · 1 Feb 2026 · 2,025 accessesView PDFCite
Research ArticleOpen accessIn the abstractPolygonatum sibiricum Red. With Steaming Yellow Rice Wine: Processing Technology, the Content of 5 Hydroxymethylfurfura and Safety AssessmentShiyu Ji, Shuo Li et al.To investigate the content and safety evaluation of 5 Hydroxymethylfurfural (5-HMF) in the processing of Polygonatum sibiricum Red, the steaming technology was optimized by response surface method combined with analytic hierarchy process of multiple indexes. The content of 5-HMF was quantitatively determined by HPLC. Safety assessment was conducted using a zebrafish model. The optimized conditions were a steaming time of … …American Journal of Biochemistry and Biotechnology, Vol. 21, Issue 2 · 20 Nov 2025 · 2,587 accessesView PDFCite
Research ArticleOpen accessIn the abstractAcaricidal Activity of Azadirachta indica (Neem Tree) and Carica papaya (Papaya) Leaf Extracts against Rhipicephalus sanguineus (Dog Ticks) Using Spray MethodAlfredo Vita Corpuz, Carmela Montoya Florentino, Paul Erman Tabuloc Quilana et al.Ticks pose a serious threat to dogs, with synthetic acaricides leading to environmental impact and resistance concerns. While Azadirachta indica and Carica papaya have shown acaricidal potential against Rhipicephalus sanguineus, their efficacy against dog ticks has not been thoroughly investigated. This study determined the acaricidal activity of A. indica and C. papaya leaf extract against R. sanguineus, both individually and … …OnLine Journal of Biological Sciences, Vol. 25, Issue 2 · 22 Apr 2025 · 8,409 accessesView PDFCite
Research ArticleOpen accessIn the abstractStudies on Pharmacokinetics and Liver Microsome Metabolism of Nuciferine in Different Species of AnimalsDong Xiao-Chen, Liu Ming-Qi, Liu Jun et al.This study aimed to compare the characteristics of pharmacokinetics and liver microsome metabolism among different species of animals including rabbit, rat and mouse and healthy New Zealand white rabbits, SPF Sprague Dawley (SD) rats and ICR mice were given intraperitoneal injection of nuciferine at a dose of 50 mg per kg body weight. Blood samples were collected via caudal vein … …American Journal of Biochemistry and Biotechnology, Vol. 17, Issue 3 · 12 Aug 2021 · 6,468 accessesView PDFCite
Research ArticleOpen accessIn the abstractOptimization of Ultrasonic-Assisted Total Flavonoids Extraction from Flower of Albizia Julibrissin Durazz. by Response Surface Methodology for Characterization of Chemical Profile and Evaluation of Antioxidant and Tyrosinase Inhibitory ActivitiesYijie Cheng, Yuwen Zhang, Hanzhang Sun et al.To investigate the flavonoids in flower of Albizia julibrissin (‘He Huan Hua’ in Chinese), the ultrasonic-assisted extraction of total flavonoids from ‘He Huan Hua’ was optimized by response surface methodology and the chemical profile was characterized by HPLC- QTOF-MS/MS. What’s more, the antioxidant and tyrosinase inhibitory activities of ‘He Huan Hua’ were also evaluated in this study. The extraction time, … …American Journal of Biochemistry and Biotechnology, Vol. 18, Issue 1 · 14 Mar 2022 · 6,733 accessesView PDFCite
Research ArticleOpen accessIn the abstractAntioxidant Activity, Anti-Inflammatory and Analgesic Effects of Caralluma europaea (Eddaghmouss) in MiceAaziz Kebbou, Mehdi Ait Laaradia, Sara Oufquir et al.The aim of the current study is to evaluate the anti-inflammatory, antioxidant and analgesic properties of ethanolic (100 and 200 mg/kg, p.o.) and ethyl acetate extracts (100 and 200 mg/kg, p.o.) of Caralluma europaea. Formalin-induced paw licking test, Acetic Acid induced Writhing Test and Hot-PlateTest were used to assess the analgesic activity. Xylene-induced ear edema test was used to evaluate … …OnLine Journal of Biological Sciences, Vol. 19, Issue 4 · 24 Dec 2019 · 7,598 accessesView PDFCite
Research ArticleOpen accessIn the abstractExtraction, Partial Identification and Bioactivities of Total Flavonoids from Carex meyeriana KunthHongbiao Wang, Penghui Wang, Xiudong Yang et al.To date, knowledge associated with extraction, chemical constituents and bioactivities of Total Flavonoids from Carex meyeriana Kunth (TFCMK) that remains unclear. Therefore, this paper was designed to optimize the ethanol reflux extraction method by response surface methodology and determine the chemical constituents by Liquid Phase-Mass Spectrometry (HPLC-MS); additionally, the bioactivities including antioxidant, antibacterial and hemolysis test were also explored. The … …American Journal of Biochemistry and Biotechnology, Vol. 15, Issue 3 · 7 Aug 2019 · 7,523 accessesView PDFCite