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24 results for “Genotyping”
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Research ArticleOpen accessEvery word in the titleIdentification of Clonal Clusters of Klebsiella pneumoniae Isolates from Chennai by Extended Spectrum Beta Lactamase Genotyping and Antibiotic Resistance Phenotyping AnalysisC. Kamatchi, H. Magesh, Uma Sekhar, Rama VaidyanathanProblem statement: An increased resistance to antibiotics has been reported in Klebsiella pneumoniae, an opportunistic gram negative pathogen belonging to Entrobacteraceae due to the evolution and spread of plasmid encoded extended spectrum beta lactamases and other genes conferring cross-resistance to other antibiotics. This is of concern due to the increasing cost of antibiotic treatment and the spread of multidrug resistance … …American Journal of Infectious Diseases, Vol. 5, Issue 2 · 30 Jun 2009 · 7,953 accessesView PDFCite
Research ArticleOpen accessEvery word in the titleMolecular Analysis of Coagulase Gene Polymorphism in Clinical Isolates of Methicilin Resistant Staphylococcus aureus by Restriction Fragment Length Polymorphism Based GenotypingM. Himabindu, D. Sugapriya Muthamilselvan, Dillip Kumar Bishi, Rama S. VermaProblem statement: To investigate coagulase gene variants through Restriction Fragment Length Polymorphism (RFLP) amongst clinical isolates of Methicillin-Resistant Staphylococcus Aureus (MRSA) collected from Chennai based hospitals. Approach: A total of 85 MRSA clinical isolates collected from various clinical sources were studied for the molecular typing purpose. To perform coagulase gene typing, the repeated units encoding hypervariable regions of the Staphylococcus … …American Journal of Infectious Diseases, Vol. 5, Issue 2 · 30 Jun 2009 · 8,766 accessesView PDFCite
Research ArticleOpen accessEvery word in the titleMolecular Genotyping of Mycobacterium tuberculosis Isolates from TurkeyBerrin Gencer, Thomas M. ShinnickTo survey the circulating strains of Mycobacterium tuberculosis in Turkey, all clinical isolates (381 patients) recovered in laboratories in six cities during one-month periods were collected and typed by IS6110-DNA fingerprinting and spoligotyping. Drug susceptibilities were also determined. About 23% of the isolates were resistant to one or more drugs and about 4% were multidrug resistant (i.e., resistant to at … …American Journal of Infectious Diseases, Vol. 1, Issue 1 · 31 Mar 2005 · 6,731 accessesView PDFCite
Research ArticleOpen accessIn the keywordsAn Effective and Viable DNA Extraction Protocol for FFPE Tissues and its Effect on Downstream Molecular ApplicationSai Sushmitha Kontham, Charles Emmanuel Jebaraj Walter, Manjula Raju et al.Archival Formalin-Fixed Paraffin-Embedded (FFPE) tissues are a readily available source of DNA for retrospective studies including genetic predisposition. FFPE tissues present significant challenges for extraction of pure genomic DNA in adequate amounts due to the harsh fixation conditions and long-term storage associated with samples. The aim of the study is to propose a simple, in-expensive, viable protocol for the extraction … …American Journal of Biochemistry and Biotechnology, Vol. 16, Issue 1 · 24 Mar 2020 · 7,374 accessesView PDFCite
Research ArticleOpen accessIn the keywordsGenetic Diversity and Drug Resistance of Mycobacterium tuberculosis Complex Isolates and Nontuberculous Mycobacteria Identification from Presumptive Tuberculosis Cases in Oaxaca, MexicoNakamura-Lopez Yuko, Valencia-Carmona Oscar Daniel, Martinez-Cruz Perla Monica et al.Tuberculosis (TB) is a re-emerging health problem worldwide. In Mexico, information about genetic diversity and drug resistance of Mycobacterium Tuberculosis complex (MTBc) is limited. Samples from 463 Presumptive TB cases were tested for TB by smear, culture and PCR, from which 19.2% were identify as MTBc and 16% as nontuberculous mycobacteria. MTBc isolates were characterized by Large Sequence Polymorphisms (LSPs), … …American Journal of Infectious Diseases, Vol. 16, Issue 1 · 9 Feb 2020 · 7,159 accessesView PDFCite
Research ArticleOpen accessIn the keywordsGENOTYPIC IDENTIFICATION OF CANDIDA SPP. ISOLATED FROM ONYCHOCANDIDIASIS PATIENTS BY PHENOTYPIC METHODS, PCR AND RAPD-PCRZaidan Khlaif Imran, H. Al-GhalibiCandidal Onychomycosis (COM) is a common nail disease which plays as sources pathogenic reservoir giving a rise to repeated candidiasis infections. This study aimed to evaluate PCR assays and phenotypic tests for identification of yeasts isolated from COM patients. The study included 100 clinically suspected patients of COM attending the main hospital and clinics in Al-Dewania province in the middle … …Current Research in Medicine, Vol. 5, Issue 1 · 31 Dec 2013 · 7,263 accessesView PDFCite
Research ArticleOpen accessIn the keywordsFirst Report of Human Immunodeficiency Virus Type 1 Circulating Recombinant Form 02_AG Recombinant Strain of African Origin from North IndiaUjjwal Neogi, Vikas Sood, Arpita Choudhury et al.Problem statement: Human Immunodeficiency Virus Type-1 (HIV-1) displays extensive genetic diversity globally which poses a serious challenge in making efficacious vaccine and developing effective therapeutic interventions. HIV-1 epidemic in India is largely driven by genetic subtype C but lately a number of reports suggest the presence of circulating recombinant forms and unique recombinant forms from different states of India. Approach: … …Current Research in Medicine, Vol. 1, Issue 2 · 31 Oct 2010 · 6,737 accessesView PDFCite
Research ArticleOpen accessIn the abstractGenome-Wide Association Study of Single Nucleotide Polymorphisms with Milk Traits in Red Gorbatov CattleMarina Ivanovna Selionova, Grigorii Sergeyevich Shekhovtsev, Ivan Petrovich Prokhorov et al.In order to preserve the gene pool of the Red Gorbatov breed, it is necessary to use a set of genomic analysis tools that allows more efficient planning in obtaining individuals who meet the economic requirements of dairy farming. The aim of the work was to identify candidate genes associated with milk production traits in Red Gorbatov cattle using a … …American Journal of Animal and Veterinary Sciences, Vol. 19, Issue 4 · 12 Oct 2024 · 4,736 accessesView PDFCite
Research ArticleOpen accessIn the abstractAnalysis of Plasma Amyloid-Beta and Apolipoprotein E Genotypes as Markers of Alzheimer’s Disease in Healthy Korean AdultsEun Ju Oh, Jung Jae Lee, Bo Kyeung Jung, Jae Kyung KimThe presence of the ApoE4 allele interferes with human Amyloid-Beta (Aβ) clearance and reduces the neuronal damage response, which, in turn, is associated with increased Alzheimer’s Disease (AD) risk. Identifying individuals with the highest genetic risk may aid clinical AD diagnosis and prognosis. However, to the best of our knowledge, no previous study has performed blood-based analysis of ApoE polymorphisms … …American Journal of Biochemistry and Biotechnology, Vol. 20, Issue 1 · 26 Mar 2024 · 4,492 accessesView PDFCite
Research ArticleOpen accessIn the abstractDistribution of Alleles and Genotypes of ACE2 Gene Polymorphisms (rs2285666 and rs35803318) and Association with COVID-19 Among Populations in KazakhstanAnar Bisseneva, Konstantin Li, Gayane Pogossyan, Valeriya ProtasThere is still no definitive confirmation regarding the association between ACE2 gene polymorphisms and COVID-19. This study was conducted to analyze the distribution of alleles and genotypes of the ACE2 gene rs2285666 and rs35803318 two polymorphisms and their possible association with COVID-19 among two ethnic groups, namely, the Kazakhs and Eastern Slavs, residing within the same geographic area in the … …OnLine Journal of Biological Sciences, Vol. 24, Issue 1 · 31 Oct 2023 · 4,942 accessesView PDFCite