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10 results for “Gene Cloning”
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Research ArticleOpen accessEvery word in the titleMolecular Cloning and Heterologous Expression of Human Interferon Alpha2b GeneI. Made Artika, Yemima Budirahardja, Anna Lucia EkowatiHuman alpha Interferons (hIFNα) have been shown to have antiviral, antiproliferative and immunomodulatory activities. The human interferon alpha2b (hIFNα2b), is one of the human interferon alpha2 sub variants, naturally synthesized as a polypeptide of 188 amino acid residues, the first 23 residues of which represents a signal peptide. In the present study, the hIFNα2b gene was expressed after being fused … …American Journal of Biochemistry and Biotechnology, Vol. 9, Issue 4 · 20 Dec 2013 · 7,835 accessesView PDFCite
Research ArticleOpen accessEvery word in the titleCloning of the Endoglucanase Gene from a Bacillus amyloliquefaciens PSM 3.1 in Escherichia coli Revealed Catalytic Triad Residues Thr-His-GluZeily Nurachman, Sari Dewi Kurniasih, Ferra Puspitawati et al.Problem statement: An Indonesian marine bacterial isolate, Bacillus amyloliquefaciens PSM 3.1 was isolated for hydrolyzing cellulose. A 1500-bp nucleotide fragment was amplified from the chromosomal DNA by the use of primers directed against the conserved sequence of Bacilli endoglucanase genes obtained from GenBank. Approach: The fragment was cloned and expressed in Escherichia coli. Results: The endoglucanase gene (eglII gene) had … …American Journal of Biochemistry and Biotechnology, Vol. 6, Issue 4 · 31 Dec 2010 · 9,724 accessesView PDFCite
Research ArticleOpen accessIn the keywordsCharacterization of Mi1.2 Whitefly (Bemisia tabaci) Resistance GeneSherin Amin Mahfouze, Shipra Saxena, Heba Amin Mahfouze, Manchikatla Venkat RajamTomato (Solanum peruvianum) Mi gene provides resistance to whitefly (Bemisia tabaci), potato aphids and nematode making Mi a useful source in integrated pest management program. The aim of this work was to isolate, clone and sequence Mi1.2 gene from S. peruvianum. In addition, physico-chemical identification of amino acids deduced from Mi1.2 gene was done. Secondary (2D) and tertiary (3D) structures … …OnLine Journal of Biological Sciences, Vol. 17, Issue 4 · 15 Nov 2017 · 7,793 accessesView PDFCite
Research ArticleOpen accessIn the abstractcDNA Cloning and Characterization of the House Dust Mite Allergen Sui p 2Joshua Evans M. Bajao, John Donnie A. RamosSuidasia pontifica (Sp) is a House Dust Mite (HDM) species found in tropical urban residential areas which causes sensitization of up to 75% of allergic patients. Cloning of allergens from Sp is a significant initial step in understanding the clinical significance of this HDM species. The study describes the cloning and in silico characterization of the group 2 allergen gene … …American Journal of Biochemistry and Biotechnology, Vol. 16, Issue 2 · 14 May 2020 · 7,885 accessesView PDFCite
Research ArticleOpen accessIn the abstractPHYLOGENETIC ANALYSIS OF THE SWINE LEUKOCYTE ANTIGEN-6 GENE GENERATED FROM SPLEENTISSUE OF KOREAN NATIVE PIGJaeyoung Kim, Hyojun Park, Yoonchung Choi, Hoyoung ChungThis study was aimed to search genetic variants, to investigate phylogenetic relationships between pig breeds and to provide basic genetic information of Korean Native Pigs (KNP) using the variations of the Swine Leukocyte Antigen-6 (SLA-6) gene. Cloning of the Swine Leukocyte Antigen (SLA)-6 gene in the MHC non-classical region was performed with spleen tissues of Korean Native Pigs (KNP). Sequencing … …American Journal of Animal and Veterinary Sciences, Vol. 9, Issue 1 · 22 Feb 2014 · 7,234 accessesView PDFCite
Research ArticleOpen accessIn the abstractGenetic Polymorphisms in the Fat Mass and Obesity-Associated Gene Confers Risk of Obesity in Iraqi PopulationShatha Ramadhanzaidan, Faris Abd Al Kareem, Lujain Anwar AlkhazrajyProblem statement: Obesity is strongly influenced by genetic factors, with an estimated heritability of 60% BMI. Genetic susceptibility to the common form of obesity appears to be polygenic. Although theoretical analyses emphasized the power of genetic association study in common polygenic diseases, the search for genes conferring the risk of obesity has thus far not been very successful. Approach: In … …American Journal of Infectious Diseases, Vol. 7, Issue 2 · 21 Sep 2011 · 7,622 accessesView PDFCite
Research ArticleOpen accessIn the abstractVibrio cholerae O1 Strains of Different Ribotypes have Similar hlyA RFLP Patterns but Different Vacuolating AbilityJorge E. Vidal, Paula Figueroa-Arredondo, Silvia Giono-Cerezo et al.Extensive cytoplasmic vacuolation on Vero and HeLa cells in vitro by the Vibrio cholerae pore forming toxin HlyA, has been previously reported by our group. Vibrio cholerae O1 and non-O1 pathogenic strains show differences in the potential to induce vacuolation, here we study occurring variations on vacuolating cytotoxic ability, related to changes in the nucleotide sequence of the hlyA-orf. A … …American Journal of Infectious Diseases, Vol. 3, Issue 2 · 30 Jun 2007 · 6,984 accessesView PDFCite
Research ArticleOpen accessAll words, across the recordCloning and Functional Analysis of tri14 in Trichoderma brevicompactumXu-Ping Shentu, Xiao-Feng Yuan, Wei-Ping Liu et al.Trichodermin, one of the trichothecenes, is a potential antifungal antibiotic and a protein synthesis inhibitor. Trichodermin is synthesized mainly by Trichoderma brevicompactum, but the biosynthetic pathway has yet been completely elucidated. Our earlier transcriptome assay on the trichodermin synthesis in T. brevicompactum 0248 identified a unique tri14 transcript, designated Tbtri14, that was co-regulated with other tri cluster genes. However, the … …American Journal of Biochemistry and Biotechnology, Vol. 11, Issue 3 · 25 Aug 2015 · 7,393 accessesView PDFCite
Research ArticleOpen accessAll words, across the recordMolecular Characterization of a Phytoplasma Associated with Coconut Yellow Decline (CYD) in MalaysiaN. Nejat, K. Sijam, S. N.A. Abdullah et al.Problem statement: Molecular methods have been used to detect phytoplasma in Malayan Red Dwarf (MRD) ecotype of coconut for the first time in Malaysia. Detect phytoplasma from coconut showing yellowing symptoms by nested PCR and Classify phytoplasma associated with disease of coconut palms, based on analysis of 16S rRNA gene operon sequences and virtual RFLP. Approach: Twenty MRD symptomatic palms … …American Journal of Applied Sciences, Vol. 6, Issue 7 · 31 Jul 2009 · 7,919 accessesView PDFCite
Research ArticleOpen accessAll words, across the recordToll-Like Receptor (TLR) 2 and TLR4 Gene Expression in Canine HeartA. Linde, F. Blecha, T. MelgarejoToll-like receptors (TLRs) are archetypal pattern recognition receptors of immediate importance for an efficacious innate immune response. TLRs exhibit marked differential tissue activity and their levels within a discrete cell type can be highly dynamic. Of 13 known mammalian paralogues, three TLRs have been identified in the dog. Although cardiac TLR expression has been reported in other species, this study … …American Journal of Animal and Veterinary Sciences, Vol. 2, Issue 1 · 31 Mar 2007 · 6,921 accessesView PDFCite