27 articles for “Enzyme Properties”

Research ArticleOpen accessIn the keywordsDiscovery of a New Thermostable Chitinase from Streptomyces No. 6Xiaowen Que, Yonghong Bao, Qing Liu et al.Chitinases are a class of hydrolases that hydrolyze the β-1, 4-glycosidic bonds between N-acetyl-D-glucosamine (GlcNAc) in chitin. Among chitinases, thermophilic chitinases and thermostable chitinases have received extensive attention in recent years due to their ability to tolerate higher temperatures and maintain enzyme stability for a longer period of time. However, it has previously been observed that not all chitinases are … …American Journal of Biochemistry and Biotechnology, Vol. 20, Issue 3 · 1 Aug 2024 · 5,115 accessesView PDF Cite

Research ArticleOpen accessIn the abstractImmunomodulatory and Antioxidant Effects of Stachys Japonica Miq. Ethanol Extract on Cyclophosphamide-Induced Immunosuppressed Mice and its Phytochemical CompositionXueping Jiang, Jianglong He, Yanjun Chen, Fang WangStachys japonica Miq. is an edible wild botanical drug and a traditional medicine in China. This study focused on examining the phytochemical profile and antioxidant properties of the ethanol extract of S. japonica Miq (SJEE) in vitro, as well as its immunomodulatory and antioxidant activities in cyclophosphamide (Cy)-induced immunosuppressed mice. The SJEE was analysed by UPLC-HRMS/MS. 36 Polyphenols, including flavonoids, … …American Journal of Biochemistry and Biotechnology, Vol. 22, Issue 1 · 13 Apr 2026 · 1,449 accessesView PDF Cite

Research ArticleOpen accessIn the abstractUnveiling the Potential of Dombeya buettneri K.Schum As a Drug Agent: Molecular Docking of Compounds With Enzymes Linked to Diabetes and Neurodegenerative DiseaseEsther Emem Nwanna, Samuel Chima Ugbaja et al.This study investigated the mechanisms of Dombeya buettneri (snowball leafy) extracts on diabetes, hypertension, and neurodegenerative diseases. It compared extracts obtained using steep water (a by-product of corn wet milling) and aqueous water. The extracts were analyzed for total phenol, total flavonoid content, antioxidant activities such as FRAP, ABTS, NO, DPPH, iron-induced lipid peroxidation assay, degradation of deoxyribose (Fenton’s reaction), … …OnLine Journal of Biological Sciences, Vol. 25, Issue 4 · 1 Feb 2026 · 2,025 accessesView PDF Cite

Research ArticleOpen accessIn the abstractA Novel Industrially Promising Keratinase Isolated from Aspergillus aureolatus Changes its Substrate Specificity after pH-Mediated AutolysisArina Mokhova, Anna ShestakovaMicrobial proteolytic enzymes are green catalysts with many potential applications. Keratinolytic enzymes (or keratinases) are crucial for efficient keratin degradation, making them essential in waste management, biomedicine, cosmetics, and various industrial applications. This study describes the production and characterization of a protease with keratinolytic activity from Aspergillus aureolatus. The protease was isolated from the culture fluid on the 5th day … …American Journal of Biochemistry and Biotechnology, Vol. 21, Issue 1 · 14 Feb 2025 · 5,580 accessesView PDF Cite

Research NotesOpen accessIn the abstractNuciferine Attenuates Cigarette Smoke-Induced Senescence in HepG-2 Cells via the miR-217/SIRT1/Nrf2 PathwayYun-Hong Zhao, Yi-Meng Ge, Wei-Jia Xu et al.The lotus leaf, widely used in both medicine and cuisine, contains bioactive compounds with therapeutic potential. Nuciferine (NF), an active constituent of significant importance, is extracted from the lotus leaf. The present study sought to uncover the molecular pathways through which Nuciferine (NF) mitigates cellular senescence triggered by Cigarette Smoke Components (CSCs) in HepG-2 cells, employing an experimental model where … …American Journal of Biochemistry and Biotechnology, Vol. 21, Issue 2 · 4 Oct 2025 · 2,296 accessesView PDF Cite

Research ArticleOpen accessIn the abstractInvestigation of the Contribution of Late Embryogenesis Abundant (LEA) K Peptide in Enhancing the Expression of Lipase from Sphingobacterium sp.: In vitro and in Silico StudiesIbrahim Muhammad et al.Sphingobacterium sp. AB3 lipase is a psychrophilic enzyme with optimal lipolytic activity at pH 7 and 15°C. The cold-adapted properties of the lipase render it suitable for various low-temperature industrial applications such as biodiesel production, detergent formulation, and non-thermal food processing. Recent studies have shown that co-expression of LEA K peptide with AB3 lipase resulted in enhanced protein expression in … …American Journal of Biochemistry and Biotechnology, Vol. 20, Issue 4 · 21 Nov 2024 · 4,569 accessesView PDF Cite

Research ArticleOpen accessIn the abstractEnhancement of Geobacillus thermodenitrificans Lipase Activity and Binding Affinity by Acetone Treatment: Kinetic and in silico InvestigationsAmmar Khazaal Kadhim Almansoori, Alyaa Abdulhussein Alsaedi et al.Lipase Geobacillus thermodenitrificans (LipGt) is a thermostable enzyme that has an optimal activity temperature of 65°C. It can tolerate various organic solvents and has an improved activity, especially in the presence of acetone. In this study, in-vitro and in-silico studies were carried out to investigate the effect of acetone in enhancing the kinetic and binding affinity of LipGt. The LipGt … …American Journal of Biochemistry and Biotechnology, Vol. 20, Issue 4 · 10 Oct 2024 · 4,817 accessesView PDF Cite

Research ArticleOpen accessIn the abstractProduction, Extraction and Characterization of Lipases from the Antarctic Yeast Guehomyces pullulansLuis Lascano-Demera, Patricia Portero Barahona, Enrique Javier Carvajal BarrigaThe production of extracellular lipases from the Antarctic yeast Guehomyces pullulans is induced using an olive oil medium as an inductor substrate and a first characterization of its enzyme, using the protein extract obtained from the medium, is described. For this, the effect of pH and temperature on the lipase activity are evaluated and the enzyme kinetic for the lipase … …American Journal of Biochemistry and Biotechnology, Vol. 15, Issue 2 · 11 Apr 2019 · 10,020 accessesView PDF Cite

Research ArticleOpen accessIn the abstractBiological Activity of Lactic Acid Based Feed Additive and its Physiological Effect on the Animal OrganismNadezhda Nikolaevna Lantseva, Alexander Nikolaevich Shvydkov, Lyudmila Anatolyevna Ryabukha, Veronica Valeryevna EvsyukovaThe data obtained as a result of the study of the enzymatic activity of the lactic acid based feed additive LAFA, based on various bacteria, are consistent with the results of the research presented in the publications on the matter, concerning the fact that probiotic bacteria have enzymatic activity. The conducted studies revealed the leading enzymatic properties of bifidobacteria in … …OnLine Journal of Biological Sciences, Vol. 18, Issue 4 · 4 Oct 2018 · 7,416 accessesView PDF Cite

Research ArticleOpen accessIn the abstractBiochemical Indicators and Nutritional Value of Horsemeat Infected by Parascaridosis and Sampled from the Surrounding Areas of Semipalatinsk Nuclear Test Site, KazakhstanShyngys Suleimenov, Sergazy Duyssembaev, Ainur Serikova et al.Due to high prevalence of the Parascaris equorum among horses, the effect of Paracardiosis infestation on nutritional and biological values of meat is required. In an effort to analyze the radionuclides contents, biochemical parameters and biological value of horsemeat collected from different areas of Semipalatinsk Nuclear Test Site (SNTS) of Kazakhstan. The prevalence of parascaridosis was performed following standard protocols. … …American Journal of Animal and Veterinary Sciences, Vol. 16, Issue 1 · 9 Feb 2021 · 6,600 accessesView PDF Cite