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72 articles for “Enzyme Concentration”
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Research ArticleOpen accessPhrase in the titleThe Influence of Trichoderma viride Cellulase Enzyme Concentration on Saccharification of Waste Paper MaterialsKhomotso M. P. Mokatse, M. Alfred Mogale, J. Pieter H. Van WykEnzymatic hydrolysis of lignocellulosic materials is one of the major steps in the commercialization process of converting cellulosic substrates into bio-products. The saccharification of lignocellulosic materials is usually achieved by a synergistic action of an enzyme mixture consisting of multiple cellulase enzymes such as endo-and exo-glucanase, cellobiohydrolase and β-glucosidase with different mode of actions. During the enzymatic hydrolysis of the … …OnLine Journal of Biological Sciences, Vol. 21, Issue 4 · 28 Oct 2021 · 6,456 accessesView PDFCite
Research ArticleOpen accessIn the abstractPotentiometric Biosensor for Highly Toxic Pb (lead) and Cd (cadmium) Detection in Milk Using Layer-by-Layer ImmobilizationZhainagul Kakimova, Klara Zharykbasova, Aitbek Kakimov et al.Milk contamination with heavy metals such as lead (Pb) and Cadmium (Cd) poses a serious threat to public health, particularly in industrial regions. To address the need for rapid and on-site detection of these contaminants, a potentiometric biosensor was developed for the quantification of Pb²⁺ and Cd²⁺ ions in milk and dairy products. The biosensor functions by measuring the change … …OnLine Journal of Biological Sciences, Vol. 25, Issue 4 · 8 Feb 2026 · 1,480 accessesView PDFCite
Research ArticleOpen accessIn the abstractIsolation and Characterization of Collagen from IndonesiaLocal Duck Feet Using Bromelain EnzymeRafika Mila Amanah, Dita Prameswari Trenggono Putri, Mohammad Zainal Abidin et al.The utilization of local duck feet as a collagen source to minimizewaste and add value to the duck by-product. This research aimed to isolate andcharacterize collagen from Local duck feet using enzymatic method. Thebromelain enzyme in various concentrations: 0% (A), 0.2% (B), 0.4%(C), 0.6%(D), and 0.8% (E). Variables observed were collagen yield, viscosity, pH,Fourier Transform Infrared Spectroscopy (FTIR), and Sodium … …American Journal of Animal and Veterinary Sciences, Vol. 20, Issue 2 · 24 May 2025 · 5,165 accessesView PDFCite
Research ArticleOpen accessIn the abstractAnalysis of Constituents and Bacteriostatic Activity of Essential Oil Extracted from Sea Buckthorn Seeds by Different MethodsHepeng Zhao, Fuyin Zhao, Qian Xiao et al.Sea buckthorn is a homologous plant listed in Chinese pharmacopoeia. The Essential Oils (EOs) of Sea Buckthorn Seeds (SBTS) are widely used in medicine and health products in China, Russia and other countries. The extraction methods of SBTS with advantages of low cost, high yield and high activity have attracted considerable attention. In this study, the extraction rate, chemical compositions … …American Journal of Biochemistry and Biotechnology, Vol. 17, Issue 2 · 4 Jun 2021 · 7,480 accessesView PDFCite
Research ArticleOpen accessIn the abstractThe Effect of Bacterial Enzyme-Based Feed Additives on the Productivity, Digestibility and Assimilation of Nutrients in Young Laying HensSmolentsev Sergei Yur’evich, Rudakova Natalia Leonidovna, Koryagina Anastasia Olegovna et al.The work aimed to study the effect of feed additives based on proteinase and phytase on the productivity, digestibility of nutrients and the development of laying hens up to 18 weeks of age. A total of 360 1-day-old Hisex Brown chickens were assigned to a completely randomized design composed of 3 diets with 4 replicates of 30 birds each. Dietary … …OnLine Journal of Biological Sciences, Vol. 20, Issue 4 · 19 Nov 2020 · 6,297 accessesView PDFCite
Research ArticleOpen accessIn the abstractCyclodextrin Glucanotransferase Activity of Microbial Strains Isolated from Yam-Cultivation Soils in Northwestern BeninCyrille Alode Vodounon, Ténor Dias-Mendel Allode et al.β-Cyclodextrin, an industrially important oligosaccharide, is produced by microbial enzymes such as cyclodextrin glucanotransferase (CGTase). This study investigated ways to optimize the enzymatic activity of CGTase from Bacillus cereus, Bacillus pumilus, Bacillus brevis, and Bacillus subtilis strains isolated from soils in north-western Benin. Cyclization activity of CGTase and the influence of pH, temperature, and metal ions were assessed using conventional … …American Journal of Biochemistry and Biotechnology, Vol. 22, Issue 3 · 21 Sep 2026 · 652 accessesView PDFCite
Research ArticleOpen accessIn the abstractMomordica balsamina Fruit Extracts Enhances Selected Aspects of the Insulin Synthesis/Secretion PathwayAnanias Hodi Kgopa, Leshweni Jerry Shai, Motetelo Alfred MogalePrevious studies have suggested that crude plant extracts of the anti-diabetic medicinal plant, Momordica balsamina (MB) may exert their anti-diabetic effect through either enhancement of insulin sensitivity or inhibition of intestinal glucose absorption. However, the effects of these MB extracts on insulin synthesis and secretion are not well documented in the literature. Therefore, a main purpose of the current research … …American Journal of Biochemistry and Biotechnology, Vol. 16, Issue 4 · 17 Dec 2020 · 6,999 accessesView PDFCite
Research ArticleOpen accessIn the abstractLarvicidal Activity of Spondias purpurea (Sineguelas) Leaf and Hylocereus costaricensis (Dragon Fruit) Stem Extracts Against Aedes aegyptiAlfredo V. Corpuz, Nigell Mikhail A. Gallardo, Ace Danielle C. AveroThe study determined the larvicidal activity of the different concentrations of Spondias purpurea and Hylocereus costaricensis extracts by measuring percent mortality in Aedes aegypti larvae, a primary vector of dengue and other arboviral diseases. The hypothesis proposed that combining both extracts would yield greater larvicidal activity due to potential synergistic interactions. Ethanolic extraction was used to isolate a wide array … …OnLine Journal of Biological Sciences, Vol. 25, Issue 3 · 20 Nov 2025 · 2,417 accessesView PDFCite
Research ArticleOpen accessIn the abstractBiosynthesis and Evaluation of Zinc, Copper, and Methionine Feed Supplements Using Saccharomyces cerevisiae on Duck Performance in Minimal Water SystemsHendi Setiyatwan, Muhammad Rifqi IsmirajOptimizing duck performance under minimal water conditions is essential for sustainable poultry farming. Biosynthesizing essential feed supplements like Zinc (Zn), Copper (Cu), and methionine using Saccharomyces cerevisiae presents a promising method to enhance growth and meat quality. This study developed a biosynthesis method for Zn, Cu, and methionine supplements using S. cerevisiae and evaluated their effects on duck performance. In … …American Journal of Animal and Veterinary Sciences, Vol. 20, Issue 1 · 11 Feb 2025 · 4,405 accessesView PDFCite
Research ArticleOpen accessIn the abstractDevelopment of an Immunomagnetic Separation Method for Viable Salmonella Typhimurium Detected by Flow CytometryShakil Ahmed, Horst-Günter Rubahn, Helmut ErdmannA very small number of bacterial pathogens may have fatal effects on food safety. In spite of having great advancements in bioanalytical methods, most of the accepted detection methods are still cultivation based and thus time consuming. This leads to an intense need for efficient and rapid methods for detection of food-related bacteria. In this study, a flow cytometry based … …OnLine Journal of Biological Sciences, Vol. 16, Issue 4 · 25 Nov 2016 · 7,574 accessesView PDFCite