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150 articles for “ELISA”
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Research ArticleOpen accessEvery word in the titleCombination of Serological Tests (Anti-CCP Antibody, Rheumatoid Factor IgM ELISA and Latex Test) are more Useful in Detection of Rheumatoid ArthritisAnindya Das, Chimanjita Phukan, Chitralekha BaruahRheumatoid arthritis is a polyarticular and chronic inflammatory disease occurring throughout the world. To prevent significant joint damage, early diagnosis and proper treatment is of paramount importance. Though patients are diagnosed clinically supported by radiography and serological tests, early disease may present with non-specific arthritis and absence of specific radiographic findings. Though anti-CCP antibody is used for the diagnosis and … …American Journal of Immunology, Vol. 13, Issue 3 · 23 Sep 2017 · 7,389 accessesView PDFCite
Research ArticleOpen accessEvery word in the titleELISA-Based Development of Functional Antibodies for the Efficient Detection of Mycotoxigenic Fusarium (F. oxysporum and F. circinatum) and Diplodia (D. pinea) SpeciesPhumzile Mkhize, Phetole MangenaFusarium contains the most toxigenic and important fungal pathogens, producing a wide range of mycotoxins that include the more toxic secondary metabolites called fumonisins. This study analyzed the presence of common exo- and mycelium-soluble antigens, purified proteins using reducing SDS-PAGE and the expression of antibodies against different fungal pathogens. The results showed the presence of common antigens between pathogens and … …American Journal of Biochemistry and Biotechnology, Vol. 16, Issue 4 · 18 Nov 2020 · 6,601 accessesView PDFCite
Research ArticleOpen accessIn the keywordsSerological and Molecular Investigation of Middle East Respiratory Syndrome Coronavirus and Equine Herpesviruses in Horses at Southwestern Saudi ArabiaAli S. AlqahtaniHost reservoirs other than dromedaries for the Middle East Respiratory Syndrome Coronavirus (MERS-CoV) still to be unknown. Equine Herpesviruses (EHVs) are widespread in equine populations worldwide. In this study, the presence of MERS-CoV, Equine Herpesviruses type 1 (EHV-1) and 4 (EHV-4) in horses in Najran, southwestern of Saudi Arabia was investigated. One hundred and thirty four blood samples collected from … …American Journal of Animal and Veterinary Sciences, Vol. 15, Issue 3 · 29 Sep 2020 · 6,602 accessesView PDFCite
Research ArticleOpen accessIn the keywordsStudy on Sero-Prevalence of Small Ruminant and Human Brucellosis in Yabello and Dire Districts of Borena Zone Oromia Regional State, EthiopiaWubishet ZewdieCross-sectional study was conducted in Yabello and Dire districts of Borena zone Oromia regional state, Ethiopia to estimate sero-prevalence of small ruminant and human Brucellosis. Sero-survey was applied to the target population of 228 small ruminants and 86 humans. The sera samples were collected from small ruminants (sheep and goats) and humans for serological analysis of brucellosis. Samples were serially … …American Journal of Animal and Veterinary Sciences, Vol. 15, Issue 1 · 21 Jan 2020 · 7,987 accessesView PDFCite
Research ArticleOpen accessIn the keywordsAssociation of Reproductive Problems and Dairy Cow Productivity in a Farm with an Outbreak of Neosporosis Abortion in BrazilFrancisco Carlos Rodrigues de Oliveira, Samira Salim Mello Gallo, Murilo de Oliveira Bôa-Morte, Nicole Brand EderliNeosporosis is a disease caused by the protozoan Neospora caninum and its only clinical symptom in adult cows is abortion. In order to determine whether N. caninum was associated with an outbreak of abortion on a high milk production dairy farm with excellent livestock and health control standards, blood was collected from all the animals in the herd to obtain … …American Journal of Animal and Veterinary Sciences, Vol. 14, Issue 1 · 1 Feb 2019 · 7,459 accessesView PDFCite
Research ArticleOpen accessIn the keywordsIncidence of Cystic Echinococcosis in Libya: I. Seroprevalence of Hydatid Disease in Sheep and Goats Naturally Exposed to the Infection in the North Midland RegionMohamed M. Ibrahem, Badereddin B. Annajar, Wafa M. IbrahemHydatid disease is one of the most and serious public health and veterinary problems in Libya and other North African countries. Thirteen rural villages of two main districts bordered to each other at the north midland of the country namely, Misrata which is almost agricultural area and about 200 km east of Tripoli and Sirt which is almost pasture area … …American Journal of Animal and Veterinary Sciences, Vol. 11, Issue 3 · 28 Oct 2016 · 7,418 accessesView PDFCite
Research ArticleOpen accessIn the keywordsA Kit for Multiplexed Detection of Antibodies to Agents of Hemotransmissible InfectionsAlexander Poltavchenko, Anna Ersh, Pavel Filatov et al.The paper presents the results of laboratory trials of method using complex initial testing of antibodies to six agents of hemotransmissible infections: HIV, hepatitis B and C viruses, cytomegalovirus, Treponema pallidum and Toxoplasma gondii. The method is based on multiplexed dot immunoassay using plane protein arrays (immunochips) with the use of colloidal gold conjugates and silver development. We describe composition … …OnLine Journal of Biological Sciences, Vol. 16, Issue 3 · 6 Sep 2016 · 7,165 accessesView PDFCite
Research ArticleOpen accessIn the keywordsOccurrence of Feline Immunodeficiency Virus (FIV) and Feline Leukemia (FeLV) in São Luís-MANathálya dos Santos Martins, Ana Paula Sousa Rodrigues, Stefanne Aparecida Gonçalves et al.The aim of this study was to evaluate the occurrence of feline immunodeficiency virus and feline leukemia virus in the city of São Luí­s, Maranhão state (MA), Brazil. A total of 120 blood samples were tested using the Polymerase Chain Reaction (PCR) to detect the proviral DNA, with an amplification of a fragment of 244bp of the FIV's gene gag. … …American Journal of Animal and Veterinary Sciences, Vol. 10, Issue 3 · 24 Jul 2015 · 8,944 accessesView PDFCite
Research ArticleOpen accessIn the keywordsSerological and Molecular Investigation of Cutaneous Leishmaniasis in Healthy Individuals from an American Cutaneous Leishmaniasis-Endemic RegionRejane Cristina Ribas-Silva, Taisa Rocha Navasconi, Laís de Souza Braga et al.American Cutaneous Leishmaniasis (ACL) is a major, clinically relevant zoonosis in Brazil. The disease spectrum includes single, localized, cutaneous ulcers, diffuse cutaneous leishmaniasis and mucosal disease. A subclinical form of the disease has also been described in individuals living in ACL-endemic regions. The goal of this study was to employ immunological and molecular diagnostic methods to evaluate the presence of … …American Journal of Infectious Diseases, Vol. 11, Issue 1 · 23 Jun 2015 · 7,324 accessesView PDFCite
Research ArticleOpen accessIn the keywordsDETECTABLE ANTI-DENGUE VIRUS IGM ANTIBODIES AMONG HEALTHY INDIVIDUALS IN OGBOMOSO, OYO STATE, NIGERIAE. K. Oladipo, C. Amanetu, T. A. Gbadero, J. K. OlokeDengue fever is a zoonosis maintained in nature by mosquitoes transmitting virus between non-human primate species, most of which develop clinically in apparent infection. It has been found to be endemic in Africa and beyond. A survey for Dengue virus IgM antibody was carried out in Ogbomoso (urban and rural areas) using Enzyme Linked Immunosorbent Assay (WKEA Med Supplies Corp, … …American Journal of Infectious Diseases, Vol. 10, Issue 2 · 3 May 2014 · 7,827 accessesView PDFCite