A Kit for Multiplexed Detection of Antibodies to Agents of Hemotransmissible Infections

The paper presents the results of laboratory trials of method using complex initial testing of antibodies to six agents of hemotransmissible infections: HIV, hepatitis B and C viruses, cytomegalovirus, Treponema pallidum and Toxoplasma gondii. The method is based on multiplexed dot immunoassay using plane protein arrays (immunochips) with the use of colloidal gold conjugates and silver development. We describe composition of the kit enabling us to conduct dot assay at room temperature within 70 min. It was shown that the results of multiplexed analysis correlate well with data obtained using commercially available monospecific kits for ELISA. Furthermore, multiplexed dot assay is faster and more efficient in execution as compared to ELISA and can be carried out in field conditions.