3 articles for “Dithiothreitol”

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Research ArticleOpen accessEvery word in the titleSupplementation with Different Concentrations of Dithiothreitol and Glutathione During Cryopreservation of Semen from Large White BoarsMahlatsana Ramaesela Ledwaba, Masindi Lottus Mphaphathi et al.The study aims to compare different concentrations (0, 2.5, 5, 7.5 and 10 mM) of antioxidants, Dithiothreitol (DTT) and Glutathione (GSH), on sperm parameters following the freeze-thawing of semen from large white boars. Three large white boars were selected for semen collection and the collected semen was sent to the lab for analysis. Following dilution with Beltsville thawing solution and … …American Journal of Animal and Veterinary Sciences, Vol. 19, Issue 3 · 3 Jun 2024 · 5,517 accessesView PDF Cite

Research ArticleOpen accessIn the keywordsExtraction and Purification of Collagenase Enzymes: A Critical ReviewSaid M Daboor, Suzanne M Budge, Abdel E Ghaly et al.Problem statement: Enzymes have vital roles in several industrial processes (foods, cosmetics, nutraceuticals and pharmaceuticals) due to their highly selective nature and high activity at very low concentrations. Recent efforts to identify new sources of useful enzymes have been concentrated on the marine environment because of the potential to make use of processing wastes. About 35-50% of the mass of … …American Journal of Biochemistry and Biotechnology, Vol. 6, Issue 4 · 31 Dec 2010 · 14,311 accessesView PDF Cite

Research ArticleOpen accessIn the abstractCharacterization of Pullulanase Type II from Bacillus cereus H1.5Hii Siew Ling, Tau Chuan Ling, Rosfarizan Mohamad, Arbakariya B. AriffProblem statement: Pullulanase is one of the important enzymes in starch industry. Search for the pullulanase with distinct features, possibly from easily grown bacterium, is of interest for industrial applications Approach: The extracellular pullulanase produced by Bacillus cereus HI.5 was purified by chromatographic method of DEAE-Sepharose, followed by Superdex gel filtration. The enzyme was characterized in terms of the optimal … …American Journal of Biochemistry and Biotechnology, Vol. 5, Issue 4 · 31 Dec 2009 · 8,808 accessesView PDF Cite