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Research ArticleOpen accessIn the keywordsEvaluation of Transcript Labeling Techniques and Development of a Membrane-based Parallel Gene Expression AssayMark A. Fisher, Bonnie B. Plikaytis, Thomas M. ShinnickAn inexpensive alternative to micro arrays was developed to examine the expression of small sets of genes in parallel and used to compare direct and indirect transcript labeling methods. Psoralen-biotin direct labeling was ~10-fold less sensitive than reverse transcriptase-biotin-dUTP labeling, but the enzymatic method generated higher levels of background noise in miniarray hybridizations. The reproducibility of hybridization intensities was the … …American Journal of Biochemistry and Biotechnology, Vol. 1, Issue 1 · 31 Mar 2005 · 6,868 accessesView PDF Cite

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