8 results for “DNA Fragmentation”

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Research ArticleOpen accessPhrase in the titleSperm DNA Fragmentation and Apoptosis Levels: A Comparison of the Swim up and the Density Gradient Centrifugation Methods for Sperm PreparationSilvia W. Lestari, Triyana Sari, Dwi A. PujiantoThis study investigated the efficiency of two sperm preparation methods, namely, density gradient centrifugation and the swim-up method, in selecting spermatozoa based on the DNA fragmentation index and the apoptosis levels. Fifteen semen samples from infertile couples underwent sperm preparation in this study. The Makler® counting chamber and Eosin Y staining were used to analyse the sperm concentration, motility and … …OnLine Journal of Biological Sciences, Vol. 16, Issue 4 · 14 Nov 2016 · 7,419 accessesView PDF Cite

Research ArticleOpen accessPhrase in the titleBiotic and Abiotic Factors of Sperm nDNA Fragmentation in Farm AnimalsBaylar Iolchiev, Natalya Volkova, Pavel Klenovitskiy et al.Infertility and subfertility are among the global challenges of our time. Fertility in livestock farming is not only biologically important, it is also economically important. The aim of this study was to study the effect of these factors on sperm chromatin nucleus and to assess the impact of the fragmentation index degree on bull reproductive performance. The influence of abiotic … …American Journal of Animal and Veterinary Sciences, Vol. 16, Issue 1 · 11 Mar 2021 · 6,714 accessesView PDF Cite

Research ArticleOpen accessIn the keywordsUse of Quinolones in Bull Semen Extenders to Reduce Sperm Deoxyribonucleic Acid DamageClara Gonzalez-Marin, Michael Eduard Kjelland, Rosa Roy et al.Cryopreserved sperm samples from Holstein bulls (n = 20) were examined for bacterial presence and Sperm DNA Fragmentation (SDF) dynamics. SDF was assessed after thawing (T0) and at 4, 24 and 48 h of incubation (37°C) and the rate of SDF (r-SDF), as an estimator of the DNA degradation over time, was calculated. Two groups of bulls were identified based … …American Journal of Animal and Veterinary Sciences, Vol. 7, Issue 4 · 29 Dec 2012 · 7,430 accessesView PDF Cite

Research ArticleOpen accessIn the abstractApoptosis Inducer Capacity of Cardiotonic Steroids of Urginea maritima Extract on SH-SY5Y Neuroblastoma Cells, with Less Susceptibility among Neuron-Module CellsMaati M. Elghuol, Khalilah Abdul Khalil, Mazatulikhma Mat Zain, Mohamed Saifulaman Mohamed SaidNatural products have played a significant role in conventional treatment and in the development of new drugs for a wide range of illnesses, including malignant disease. Urginea maritima is classified as an herb is endemic to the Mediterranean area. This herb was renowned in traditional ethnopharmacological applications. Notwithstanding, there is a lack of evidence pertaining to the characterization of the … …American Journal of Applied Sciences, Vol. 13, Issue 5 · 28 May 2016 · 7,345 accessesView PDF Cite

Research ArticleOpen accessIn the abstractEvidences for the Role of Melatonin as a Protective Additive During Buffalo Semen FreezingMohamed El-Raey, M. R. Badr, Z. M. Rawash, G. M. DarwishTill now, the exact role of melatonin in male infertility hasn’t been fully discovered. Moreover, the intracellular signaling pathways activated by melatonin in buffalo spermatozoa especially on the level of cryopreservation process haven’t been reported. The current study aimed simply to clarify the precise role of this hormone during buffalo semen cryopreservation. Semen samples were obtained randomly from 6 fertile … …American Journal of Animal and Veterinary Sciences, Vol. 9, Issue 4 · 23 Dec 2014 · 8,112 accessesView PDF Cite

Research ArticleOpen accessIn the abstractEmetine produce Entamoeba histolytica Death by Inducing a Programmed Cell DeathJ.D. Villalba-Magdaleno, R. Rojas, C. Gómez et al.Amoebiasis is a major public health problem in tropical and subtropical countries. Although a number of antiamoebic agents are used for its treatment and the molecular targets of these drugs have been identified, the biochemical and physiological mechanisms that produce trophozoite death are little known. The present study dates the in vitro induction of programmed cell death (PCD) in E. … …American Journal of Infectious Diseases, Vol. 3, Issue 2 · 30 Jun 2007 · 7,211 accessesView PDF Cite

Research ArticleOpen accessIn the abstractEvidences of Apoptosis in Hepatitis C Virus-Infected Hepatocytes and Peripheral Blood Monocuclear Cells in the Absence of Liver InjuryViviana Falcón, Nelson Acosta-Rivero, Mineko Shibayama et al.Understanding the mechanism of Hepatitis C Virus (HCV) pathogenesis is an important part of HCV research. In this study, the presence of apoptosis in HCV-infected liver and Peripheral Blood Mononuclear Cells (PBMC) from patients positive for anti-HCV antibodies and negative for serum HCV-RNA was investigated. The samples obtained from 21 patients were studied by in situ Hybridization (ISH), Immunofluorescence, TUNEL … …American Journal of Infectious Diseases, Vol. 1, Issue 1 · 31 Mar 2005 · 6,975 accessesView PDF Cite

Research ArticleOpen accessIn the abstractDetection of HCV Components and Pathological Reactions in Apoptotic Hepatocytes from Chronically HCV-infected PatientsViviana Falcón, Nelson Acosta-Rivero, Mineko Shibayama et al.Analysis of Hepatitis C Virus (HCV)-infected hepatocytes at the cellular level may contribute to elucidate the mechanisms of HCV pathogenesis. In this work, the presence of HCV components and pathological reactions in apoptotic hepatocytes from chronic HCV-infected patients were studied by electron microscopy and confocal microscopy. Eight samples of liver biopsies from patients with chronic hepatitis C were studied by … …American Journal of Infectious Diseases, Vol. 1, Issue 1 · 31 Mar 2005 · 6,907 accessesView PDF Cite