4 articles for “Cryoprotectants”

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Research ArticleOpen accessEvery word in the titleAugmentation of Temperatures, Media and Cryoprotectants on in vitro Fertilization of Pig OocytesTumelo Thelma Maduwa, Masindi Lottus Mphaphathi et al.There are numerous challenges to the large-scale production of pig embryos using in vitro procedures. The aim of the present study was to compare the different holding temperatures, in vitro maturation media, and permeating cryoprotectants in the in vitro fertilization of pig oocytes. Ovaries were obtained from the local slaughterhouse, oocytes were retrieved by means of aspiration and slicing techniques … …American Journal of Animal and Veterinary Sciences, Vol. 19, Issue 3 · 29 Aug 2024 · 4,650 accessesView PDF Cite

Research ArticleOpen accessEvery word in the titleEffect of two Different Permitting and Combination of Cryoprotectants on Cattle Oocytes Maturation Rate Following Brilliant Cresyl Blue ExposureMasindi Lottus Mphaphathi, Hester Adri O'Neill, Tshimangadzo Lucky NedambaleThe objectives of this study were to elucidate the toxicity of Dimethyl sulfoxide (DMSO), Ethylene glycol (EG), and the combination of both permitting cryoprotectants (CPAs) to cattle oocytes and the effectiveness of Brilliant cresyl blue (BCB) on immature oocytes pre-selection. Cattle ovaries were collected from the local slaughterhouse and transported within 2 hours of slaughter. The oocytes were exposed to … …American Journal of Animal and Veterinary Sciences, Vol. 17, Issue 2 · 6 May 2022 · 6,201 accessesView PDF Cite

Research ArticleOpen accessIn the keywordsBiological Study Scabiosa ochroleuca Seeds Germination and Development of Methods for a Long-Term StorageSayagul Serikovna Tyrzhanova, Margarita Yulaevna Ishmuratova, Sayran Nikolayevna Atikeeva et al.The organization of a storage system for seeds of medicinal plants is an important task of introduction. Our study aimed to identify biological features during the germination of Scabiosa ochroleuca seeds and optimize the conditions for their storage and germination activation. The paper presents the results of assessing the viability of seeds of S. ochroleuca depending on the origin, size, … …OnLine Journal of Biological Sciences, Vol. 22, Issue 4 · 26 Nov 2022 · 5,089 accessesView PDF Cite

Research ArticleOpen accessIn the abstractUse of Cooled Bull Semen as a Strategy to Increase the Prenancy Rate in Fixed-Time Artificial Insemination Prgrams-Case ReportAndre Maciel Crespilho, Frederico Ozanam Papa, Marcos de Paula Santos, Manoel Francisco de Sa FilhoSemen cryopreservation is still considered suboptimal due to lower fertility when compared to fresh semen. The reasons for the loss of fertility are various and related to irreversible damage caused to the cells during the freeze-thaw process. An alternative to conventional cryopreservation represents the use of chilled bull semen, preventing the damage associated with freezing, thereby guaranteeing greater sperm viability. … …American Journal of Animal and Veterinary Sciences, Vol. 7, Issue 4 · 2 Feb 2013 · 8,681 accessesView PDF Cite