2 articles for “Cre/loxp”

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Research ArticleOpen accessIn the keywordsKnock Down of Erythrulose Kinase (eyk1) Leads to the Enhancement of Erythritol Production in Yarrowia lipolytica Mutant Strain YE4-2Xinhe Zhao, Tianlong Huang, Yaxin Zhang et al.Erythritol is a nearly zero-calorie sweetener, which is in great demand in food and health care industry. Our previous work obtained a Yarrowia lipolytica mutant strain YE4-2 with high erythritol yield. However, its conversion rate of glucose needs further improvement. Erythrulose kinase (eyk1) participates in the early stages of erythritol catabolism; the existence of eyk1 prevents the accumulation of erythritol. … …American Journal of Biochemistry and Biotechnology, Vol. 16, Issue 3 · 24 Jul 2020 · 6,750 accessesView PDF Cite

Research ArticleOpen accessIn the abstractTransformation of Lesquerella Fendleri with the New Binary Vector pGPro4-35SGrace Q. Chen, Roger Thilmony, Jiann-Tsyh LinProblem statement: Crop genetic engineering requires the use of various promoters to control the expression of introduced transgenes. Some of the binary vectors currently available for promoter characterization in dicotyledonous plants have pitfalls due to their construction, such as containing a selectable marker cassette with enhancer sequences that can potentially interfere with the expression specificity of nearby promoters. Also, many … …OnLine Journal of Biological Sciences, Vol. 11, Issue 3 · 19 Jan 2012 · 7,425 accessesView PDF Cite