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6 results for “Cellobiohydrolase”
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Research ArticleOpen accessEvery word in the titleEffect of Adding Cellulose on Lipid Accumulation in Cellobiohydrolase and Delta-6 Desaturase Engineered Mucor circinelloides StrainsYao Zhang, Yanxia Wang et al.Despite microbial production of multifunctional gamma-linolenic acid (GLA) being an important and promising way, the high cost is still a major factor limiting industrial applications. Previously, we proposed a novel approach to promote the production of GLA from cellulose, a widely available and cheapest resource, by overexpression of the cellobiohydrolase (CBH2) and delta-6 desaturase (D6) in Mucor circinelloides. In the … …American Journal of Biochemistry and Biotechnology, Vol. 19, Issue 3 · 13 Oct 2023 · 4,731 accessesView PDFCite
Research ArticleOpen accessIn the keywordsCloning of the Endoglucanase Gene from a Bacillus amyloliquefaciens PSM 3.1 in Escherichia coli Revealed Catalytic Triad Residues Thr-His-GluZeily Nurachman, Sari Dewi Kurniasih, Ferra Puspitawati et al.Problem statement: An Indonesian marine bacterial isolate, Bacillus amyloliquefaciens PSM 3.1 was isolated for hydrolyzing cellulose. A 1500-bp nucleotide fragment was amplified from the chromosomal DNA by the use of primers directed against the conserved sequence of Bacilli endoglucanase genes obtained from GenBank. Approach: The fragment was cloned and expressed in Escherichia coli. Results: The endoglucanase gene (eglII gene) had … …American Journal of Biochemistry and Biotechnology, Vol. 6, Issue 4 · 31 Dec 2010 · 9,724 accessesView PDFCite
Research ArticleOpen accessIn the abstractEffects of Soil Storage at Freezing Temperatures on Soil Enzymatic ActivitiesAinsley Lougheed, Kabwe NkongoloSoil enzyme activities are good indicators of soil health. It has been hypothesized that soil storage before analysis might affect microbial function. The objective of the present study was to determine if soil storage at -20 and -80°C affects soil enzymatic activities. Soil samples were collected and stored for four weeks at -20 and -80°C. Activities of nine enzymes were … …American Journal of Biochemistry and Biotechnology, Vol. 20, Issue 3 · 12 Aug 2024 · 4,984 accessesView PDFCite
Research ArticleOpen accessIn the abstractInfluence of Decreasing Temperature on Soil Microbial Activity in a Boreal Shield EcozoneAlistar Moy, Kabwe NkongoloAlthough the focus on warming is imperative to address climate change, addressing the effects of cooling is arguably just as relevant in characterizing the enzyme response to temperature fluctuations. In this study, the response of enzyme activity to diminishing temperatures in the field was evaluated. The targeted sites were located in the City of Greater Sudbury in Northern Ontario, Canada. … …American Journal of Environmental Sciences, Vol. 18, Issue 2 · 17 Apr 2022 · 6,660 accessesView PDFCite
Research ArticleOpen accessIn the abstractThe Influence of Trichoderma viride Cellulase Enzyme Concentration on Saccharification of Waste Paper MaterialsKhomotso M. P. Mokatse, M. Alfred Mogale, J. Pieter H. Van WykEnzymatic hydrolysis of lignocellulosic materials is one of the major steps in the commercialization process of converting cellulosic substrates into bio-products. The saccharification of lignocellulosic materials is usually achieved by a synergistic action of an enzyme mixture consisting of multiple cellulase enzymes such as endo-and exo-glucanase, cellobiohydrolase and β-glucosidase with different mode of actions. During the enzymatic hydrolysis of the … …OnLine Journal of Biological Sciences, Vol. 21, Issue 4 · 28 Oct 2021 · 6,456 accessesView PDFCite
Research ArticleOpen accessIn the abstractBrevibacillus Thermoruber 9X-GLC, Bacteria Isolated from Hot Compost, Producer of a Beta-Glucosidase Resistant to Glucose InhibitionAlejandro Sosa-Peinado, María Eugenia Hidalgo-Lara, Miguel Ángel Plascencia-Espinosa et al.The thermotolerant strain Brevibacillus thermoruber 9X-GLC produces large amounts of β-D-glucosidase and cellobiohydrolase when grown on cellobiose and avicel. 9X-GLC was isolated from hot compost of sugarcane bagasse and filter mud and selected for its high enzymatic activity from a Bacilli collection of 77 isolates from soils and compost. Culture supernatants and ultrafiltered concentrates from shaken flask cultures of 9X-GLC … …American Journal of Biochemistry and Biotechnology, Vol. 13, Issue 4 · 20 Oct 2017 · 8,153 accessesView PDFCite