86 articles for “Cell Culture”

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Research ArticleOpen accessPhrase in the titleMarine-Derived Extracts as Economically Sustainable and Ethical Alternatives to Fetal Bovine Serum in HEK293 Cell CultureKian Kenneth Francisco Sanchez, Jennifer Seki-Wong et al.Fetal Bovine Serum (FBS) is a staple supplement in cell culture, providing key growth factors that promote cell health and proliferation. Since 1885, cell culture has been a cornerstone of both basic and applied research, leading to significant discoveries of cell communication and function. However, the use of FBS presents economic and ethical challenges. Millions of bovine fetuses are euthanized … …American Journal of Biochemistry and Biotechnology, Vol. 22, Issue 3 · 7 Aug 2026 · 1,042 accessesView PDF Cite

Research ArticleOpen accessPhrase in the titleEffect of Elicitors and Precursors on the Synthesis of Anthocyanin in Grape Vitis vinifera Cell CulturesNay Min Min Thaw Saw, Heidi Riedel, Onur Kütük et al.Problem statement: Nowadays, plant cell suspension cultures and immobilized cells are being utilized for the higher yield and quality of the products than extraction of whole plants. Anthocyanins are compounds found in plants that have powerful antioxidant properties. They also provide some of the coloring or pigment of plants, flowers and fruits. Anthocyanin from grape cell cultures can be used … …Energy Research Journal, Vol. 1, Issue 2 · 31 Oct 2010 · 15,277 accessesView PDF Cite

Research ArticleOpen accessPhrase in the titleIndications of Potential Toxic/Mutagenic Effects of World Trade Center Dust on Human Lung Cell CulturesConstantino G. Lambroussis, Barbara D. Soares, Sergio Perez et al.Problem statement: Respiratory complications have been linked with exposure to dust particulates after the tragedy of September 11th, 2001 at the World Trade Center (WTC) site. Approach: The purpose of these experiments was to investigate the extent of cellular damage resulting from WTC dust exposure. Results: This research project was conducted with human lung fibroblast cells exposed to WTC dust. … …OnLine Journal of Biological Sciences, Vol. 9, Issue 3 · 30 Sep 2009 · 7,394 accessesView PDF Cite

Research ArticleOpen accessPhrase in the titleDevelopment of Growth Medium for Centella Asiatica Cell Culture Via Response Surface MethodologyRozita Omar, M. A. Abdullah, M. A. Hasan, M. MarziahThe effects of sucrose, Indole-3-Acetic Acid (IAA) and 6-BenzylAminoPurine (BAP) concentrations on cell growth of Centella asiatica cell suspension culture were studied. The concentrations were designed using Central-composite experimental design and regression analysis was carried out to obtain response surface model describing cell growth for prediction of optimum conditions. Only sucrose as a single factor was positively significant for cell … …American Journal of Applied Sciences, Vol. 1, Issue 3 · 30 Sep 2004 · 7,204 accessesView PDF Cite

Research ArticleOpen accessPhrase in the titleBlue and White LED Lights Enhance Biosynthesis of Rosmarinic Acid in Cell Culture of Agastache rugosaWoo Tae Park, Sun Kyung Yeo, Nam Su Kim et al.Agastache rugosa, an herbaceous Korean perennial, contains pharmacologically important phenylpropanoids such as tilianin and rosmarinic acid. However, the knowledge of biosynthesis of phenylpropanoids from A. rugosa is limited. Therefore, a study was conducted to develop an efficient protocol for in vitro regeneration of A. rugosa and to investigate the influence of three different LED light wavelengths (white, blue and red) … …OnLine Journal of Biological Sciences, Vol. 20, Issue 2 · 24 May 2020 · 6,860 accessesView PDF Cite

Research ArticleOpen accessEvery word in the titleComparative Hematological Values, Morphometric and Morphological Observation of the Blood Cell in Capture and Culture Asian Eel, Monopterus albus (Zuiew)Siripan Ponsen, Nual-Anong Narkkong, Supaporn Pamok, Worapol AengwanichProblem statement: Monopterus albus (Zuiew), Asian Eel, is one of the common fish found mainly in Asia. At present, the habitats of Asian Eels have been on the decrease. Contrarily, consumption of Asian Eel has increased. Whereas, data regarding blood cell characteristics, blood cell sizes and hematological values of Asian Eel are limited. As such, the objective of this study … …American Journal of Animal and Veterinary Sciences, Vol. 4, Issue 2 · 30 Dec 2009 · 7,801 accessesView PDF Cite

Research ArticleOpen accessIn the keywordsPrenatal Ethanol Exposure Affects the Proliferation and Differentiation of the Osteoblasts from Newborn RatsIsabel Chaves Silva Carvalho, Dennia Perez de Andrade, Noala Vicensoto Moreira Milhan et al.Alcohol exerts teratogenic effects and its consumption during pregnancy may cause various alterations in the fetus, including deficit of bone development. The objective of this study was to evaluate the initial responses, on osteoblasts isolated from newborn rat calvaria, after prenatal ethanol exposure. Nine pregnant rats were divided into three groups according to the diet fed during pregnancy: Rats fed … …OnLine Journal of Biological Sciences, Vol. 15, Issue 3 · 14 Jul 2015 · 6,704 accessesView PDF Cite

Research ArticleOpen accessIn the keywordsEffect of Plant Growth Regulators on Callus, Cell Suspension and Cell Line Selection for Flavonoid Production from Pegaga (centella asiatica L. urban)Suat Hian Tan, Radzali Musa, Arbakariya Ariff, Mahmood MaziahProblem statement: Considering pegaga medicinal properties and over-exploitation, the requirement for a tissue culture technique as an alternative production system was crucial. Approach: Investigation of cell suspension culture response to different plant growth regulators (PRGs) for flavonoid production from elite cell line was carried out. Callus cultures were initiated from the leaf explants of Centella asiatica on Murashige and Skoog … …American Journal of Biochemistry and Biotechnology, Vol. 6, Issue 4 · 31 Dec 2010 · 11,254 accessesView PDF Cite

Research ArticleOpen accessIn the keywordsThe Participation of Oxidative Stress in Breast Cancer Cells Progression and Treatment ResistancePoliana Camila Marinello, Kaliana Larissa Machado, Rubens Cecchini, Alessandra Lourenço CecchiniThis article presents a general discussion about the participation of oxidative stress in relevant points related to breast cancer progression in vitro. All of the evidences presented here are based on published papers that used breast cancer cells with different phenotype characteristics in their research. We observed that oxidative stress could modulate by several manners the tumor progression and these … …American Journal of Immunology, Vol. 10, Issue 4 · 17 Jan 2015 · 7,511 accessesView PDF Cite

Research ArticleOpen accessIn the keywordsThe Effect of Salivary Gland Extract of Lucillia Sericata Maggots on the Human Dermal Fibroblasts ProliferationSimona Polakovicova, Stefan Polak, Marcela Kuniakova et al.Problem statement: The aim of study was to investigate the effect of Salivary Gland Extract (SGE) of Lucillia sericata maggots on Human Neonatal Fibroblasts (B-HNF-1) proliferation and migration within collagen/hyaluronan membrane. Chronic, non-healing wounds are major health care problem worldwide. Nowadays, Maggot Debridement Therapy (MDT) has attracted much attention due to its successfull application in a treatment of chronic wounds … …OnLine Journal of Biological Sciences, Vol. 11, Issue 3 · 19 Jan 2012 · 7,641 accessesView PDF Cite