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92 results for “Incubation”

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Research ArticleOpen accessIn the abstractCharacterization and Wash Performance Analysis of Microbial Extracellular Enzymes from East Calcutta Wetland in IndiaRamesh Malathu, Sanhita Chowdhury, Madhusmita Mishra et al.Extracellular protease from a novel bacterial isolate showing maximum similarity of 98.22% with Microbacterium luteolum was obtained from East Calcutta Wetland, India. It showed compatibility with commercial detergents. The enzyme retains more than 60% of its activity between 6.0 to 10.5 pH. The maximum activity is at pH 7.5 with 71% activity at pH 10.0 and 10.5. The protease retained … …American Journal of Applied Sciences, Vol. 5, Issue 12 · 31 Dec 2008 · 8,731 accessesView PDFCite
Research ArticleOpen accessIn the abstractDistillation and Microdiffusion Modifications for total Nh4f Quantification and 15N RecoveryGaius D. Eudoxie, Gregory A. GouveiaProblem statement: Applying 15N techniques to accurately determine the fate of fixed ammonium (NH4f) in the strongly and weakly held pools require modifications to existing methodologies. Modifications are necessary for measurement of total NH4f in soils by direct digestion with 5 M HF: 1 M HCl, excluding alkali pretreatment, followed by distillation and quantification of NH4. Quantification by distillation was … …American Journal of Agricultural and Biological Sciences, Vol. 3, Issue 4 · 31 Dec 2008 · 7,593 accessesView PDFCite
Research ArticleOpen accessIn the abstractLaboratory Investigation of the Biology of Bactericera tremblayi Wag. (Homoptera: Triozidae) a New Pest in Onion Fields of IranMohammad Hossein. Kazemi, Mohammad Mashhadi JafarlooProblem statement: Psyllids as a small group of insects with plant feeding adult and nymphal stages, not only could have direct feeding damages but they could also transmit plant diseases especially viruses. Bactericera tremblayi had recently increased to high densities in onion fields in East Azarbaijan province in Iran and the pest had become widespread. This was the first study … …American Journal of Agricultural and Biological Sciences, Vol. 3, Issue 4 · 31 Dec 2008 · 7,505 accessesView PDFCite
Research ArticleOpen accessIn the abstractDegradability Characteristics of Treated and Untreated Barley Grain Using In situ TechniqueAkbar Taghizadeh, Nemati ZabihollahThis study was carried out to determine of degradability characteristics of untreated barely grain (UBG) and treated barley grain with autoclaving at 120°C, 5´ (TBG1) and 20' (TBG2), treated barley grain at 100°C, 5´ (TBG3) and 20' (TBG4), using in situ technique in Ghizel sheep’s. The sheep fed diet content 40% alfalfa: 60% concentrate containing 2.9 Mcal kg−1 DM and … …American Journal of Animal and Veterinary Sciences, Vol. 3, Issue 2 · 30 Jun 2008 · 8,308 accessesView PDFCite
Research ArticleOpen accessIn the abstractNutrient Digestibility and Gas Production of Some Tropical Feeds Used in Ruminant Diets Estimated by the in vivo and in vitro Gas Production TechniquesPaya Hamid, Taghizadeh Akbar, Janmohammadi Hossein, Moghadam Gholam AliSome feedstuffs which used in ruminants diet (corn grain, soybean meal, wheat bran and alfalfa) were analyzed for chemical composition, apparent in vivo nutrient digestibility, in vitro fermentation gas production and metabolizable energy. Chemical composition of test feeds differed in nutrient contents. Initially apparent in vivo digestibility of alfalfa nutrients were obtained then digestibility of nutrients for the other test … …American Journal of Animal and Veterinary Sciences, Vol. 2, Issue 4 · 31 Dec 2007 · 7,672 accessesView PDFCite
Research ArticleOpen accessIn the abstractEffect of Fig (Ficus carica) Leaf Extract on the Secretion and Content of Cholesterol in Hepg2 CellAhmad Fatemi, Ali Rasouli, Farzad AsadiTraditional medicines remain a source of potential for discovering of new compounds with valuable pharmacological activities. Leaves of Ficus carica were dried, powdered and extracted using methanol (ME).An aliquot of ME was dried and re-extracted by water:chloroform and the other aliquot by water: peteruleum ether. Effect of aqueous fractions of the former (ACR; 0.08, 0.1 and 0.13 mg dL-1), the … …American Journal of Animal and Veterinary Sciences, Vol. 2, Issue 4 · 31 Dec 2007 · 7,423 accessesView PDFCite
Research ArticleOpen accessIn the abstractCardiovascular Effect of Capparis spinosa Aqueous Extract. Part VI: in vitro Vasorelaxant EffectN. A. Zeggwagh, J. B. Michel, M. EddouksThe aim of this study was to investigate the in vitro vasorelaxanteffect of Capparis spinosa aqueous extract (CSAE) at a dose of 10 mg mL-1. Aortic rings isolated from normal wistar rats were used. The addition of CSAE during the plateau phase of contraction, induced by noradrenaline and KCl, produced a rapid relaxation. Incubation of aortic ring with CSAE during … …American Journal of Pharmacology and Toxicology, Vol. 2, Issue 3 · 30 Sep 2007 · 7,449 accessesView PDFCite
Research ArticleOpen accessIn the abstractImmobilization of Lipase from Candida Rugosa on Palm-Based Polyurethane Foam as a Support MaterialRoila Awang, Mohd Rafaei Ghazuli, Mahiran BasriLipase from Candida rugosa was immobilized onto palm-based polyurethane foam (PU), which the polymer was pre-soaked in co-immobilized agent. The activities of PU-immobilized lipase were tested by the esterification reaction of oleic acid and oleyl alcohol in hexane. The PU-immobilized lipase was then characterized in term of its thermal, operational and storage stability. The optimum temperature for native and PU-immobilized … …American Journal of Biochemistry and Biotechnology, Vol. 3, Issue 3 · 30 Sep 2007 · 7,485 accessesView PDFCite
Research ArticleOpen accessIn the abstractEmetine produce Entamoeba histolytica Death by Inducing a Programmed Cell DeathJ.D. Villalba-Magdaleno, R. Rojas, C. Gómez et al.Amoebiasis is a major public health problem in tropical and subtropical countries. Although a number of antiamoebic agents are used for its treatment and the molecular targets of these drugs have been identified, the biochemical and physiological mechanisms that produce trophozoite death are little known. The present study dates the in vitro induction of programmed cell death (PCD) in E. … …American Journal of Infectious Diseases, Vol. 3, Issue 2 · 30 Jun 2007 · 7,214 accessesView PDFCite
Research ArticleOpen accessIn the abstractInoculation of a Poultry Isolate Salmonella enteritidis on Egg Vitelline Membrane: Survival and Growth in Egg Components after Different Refrigeration Storage TimesZ.R. Howard, R.W. Moore, I.B. Zabala Diaz et al.An in vitro study was designed to determine the extent of Salmonella enterica serovar enteritidis survival and growth permissiveness in egg components isolated from shell eggs held at refrigeration temperature over an 8 week time period. Eggs were collected from a commercial laying facility at one-week intervals for eight weeks and stored at refrigeration temperature. After storage, eggs were dipped … …American Journal of Agricultural and Biological Sciences, Vol. 2, Issue 2 · 30 Jun 2007 · 7,664 accessesView PDFCite