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Research ArticleOpen accessIn the abstractThe Application of Electrochemical Impedance Techniques in Analyzing the AC Response of Some Two-electron Transfer Dye SystemsFarouk RashwanThe Electrochemical Impedance Spectroscopic techniques (EIS) were used to investigate the behavior of some dye compounds (quinoid systems) characterized with 2e-transfer processes. For this purpose, Alizarin Red S (ARS), Alizarin Cyanine (AC), Alizarin Viridin (AV) and carminic acid were chosen for the measurements. The EIS experiments were performed using a small AC amplitude (10 mV p-p) in addition to a … …American Journal of Applied Sciences, Vol. 2, Issue 12 · 31 Dec 2005 · 6,780 accessesView PDFCite
Research ArticleOpen accessIn the abstractPhytochemical Content and Antioxidant Potential of Leaf Extract Berchermia discolour (Munie Tree) For Goats in The Limpopo Province, South AfricaMosima Molepo, Grace Manyelo, Busisiwe GunyaThe study aims to investigate the phytochemical content and antioxidant potential of leaf extract Berchermia discolour (Munie tree) for goats. Plant leaves were ground, and their powder was mixed with reagents and chemicals to attain the concentration level of phytochemicals and other bioactive compounds found in these plant's properties. Most of the phytoconstituents were present (shown by the change of … …OnLine Journal of Biological Sciences, Vol. 25, Issue 2 · 29 Mar 2025 · 5,357 accessesView PDFCite
Research ArticleOpen accessIn the abstractEnhancement of Geobacillus thermodenitrificans Lipase Activity and Binding Affinity by Acetone Treatment: Kinetic and in silico InvestigationsAmmar Khazaal Kadhim Almansoori, Alyaa Abdulhussein Alsaedi et al.Lipase Geobacillus thermodenitrificans (LipGt) is a thermostable enzyme that has an optimal activity temperature of 65°C. It can tolerate various organic solvents and has an improved activity, especially in the presence of acetone. In this study, in-vitro and in-silico studies were carried out to investigate the effect of acetone in enhancing the kinetic and binding affinity of LipGt. The LipGt … …American Journal of Biochemistry and Biotechnology, Vol. 20, Issue 4 · 10 Oct 2024 · 4,817 accessesView PDFCite
Literature ReviewOpen accessIn the abstractOptimization of Extraction of Cannabinoids and Flavonoids Substances from Influence Cannabis sp. and their Biological Activities before and after DecarboxylationOlga Kronusová, Michala Piklová, Kristýna Šírová et al.Inflorescence of eight different varieties of Cannabis sp. (3 hemp varieties and 5 medical cannabis varieties) were subjected to extraction with n-hexane or ethanol as representatives of non-polar and polar solvents. Crude extracts were either used directly or after decarboxylation of cannabis acids for measurement of selected biological activities-cytotoxicity against carcinogenic line B16 (musculus skin melanoma cells), cytotoxicity against Human … …American Journal of Applied Sciences, Vol. 19, Issue 1 · 26 May 2022 · 6,735 accessesView PDFCite
Research ArticleOpen accessIn the abstractIsolation and Characterization of Chemical Constituents, Cytotoxicity, Antibacterial and Antioxidant Activity of the Compounds from Leptadenia hastata Roots Extract ByIsaac John Umaru, Hauwa A. UmaruThe aim of this work was to extract, isolate and characterise the chemical constituent from Leptadenia hastata roots. Fresh roots were washed and dried and was subjected to sequential maceration in polar and non-polar solvents. And introduced into rotary evaporator to concentrate the extract. Isolated compound was subjected to Thin Layer Chromatography and spectral analysis such as column and UV-Visible … …American Journal of Biochemistry and Biotechnology, Vol. 15, Issue 4 · 24 Dec 2019 · 7,959 accessesView PDFCite
Research ArticleOpen accessIn the abstractAntifungal Potential of Leptadenia Hastata Against Some Pathogenic FungiIsaac John Umaru, Fasihuddin A. Badruddin, Henry Y. Wakawa et al.The objective of this study was to evaluate if the plant extract could provide antifungal potential against some pathogenic fungi. Extract of Dichloromethane solvents was used for disc diffusion assay. The inhibitory concentration of the extract was performed by broth dilution method and zone of inhibition was studied by disc diffusion method at the concentration of 25, 50, 100, 250, … …American Journal of Biochemistry and Biotechnology, Vol. 14, Issue 1 · 9 Mar 2018 · 10,158 accessesView PDFCite
Review ArticleOpen accessIn the abstractNASA Sees First in 2018 the Direct Proof of Ozone Hole RecoveryRelly Victoria Petrescu, Raffaella Aversa, Antonio Apicella, Florian Ion Tiberiu PetrescuFor the first time, scientists have shown through direct observations of the ozone hole by a satellite instrument, built by NASA's Jet Propulsion Laboratory in Pasadena, California, that levels of ozone-destroying chlorine are declining, resulting in less ozone depletion. Measurements show that the decline in chlorine, resulting from an international ban on chlorine-containing human-produced chemicals called Chlorofluorocarbons (CFCs), has resulted … …Journal of Aircraft and Spacecraft Technology, Vol. 2, Issue 1 · 22 Jan 2018 · 10,688 accessesView PDFCite
Research ArticleOpen accessIn the abstractHeterologous Expression and Biochemical Characterization of Lipase from Burkholderia cepacia Lu10-1Yao Zhang, Lu Wang, Yuanda SongEven though lipase genes from a variety of microorganism have been cloned and over-expressed, the prospective lipase resources for commercial production and industry application are still limited. In the present study, a lipase from Burkholderia cepacia Lu10-1 is heterologously over-expressed in Escherichia coli strain BL21(DE3) and purified to homogeneity. The molecular weight of the recombinant lipase from B. cepacia Lu10-1 … …American Journal of Biochemistry and Biotechnology, Vol. 13, Issue 4 · 23 Dec 2017 · 8,765 accessesView PDFCite
Review ArticleOpen accessIn the abstractA Review: Biodegradation and Applications of Keratin Degrading Microorganisms and Keratinolytic Enzymes, Focusing on Thermophiles and Thermostable Serine ProteasesWirojne Kanoksilapatham, Weeranut IntagunKeratins are hard-degrading fibrous proteins, insoluble in water and organic solvents, often accumulated in nature and major components in feathers, skins, hair, horn, nail, hoof etc., Keratin-degrading microorganisms such as bacteria, archaea, actinomycetes and fungi employ keratinases to attack keratin. Keratinases belonging to subtilisin-like serine proteases were classified based on similarity of amino acid sequences. Keratinolytic thermophilic or hyperthermophilic bacteria … …American Journal of Applied Sciences, Vol. 14, Issue 11 · 10 Nov 2017 · 9,981 accessesView PDFCite