87 results for “Cell Culture”

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Research ArticleOpen accessIn the abstractEvaluation of Transcript Labeling Techniques and Development of a Membrane-based Parallel Gene Expression AssayMark A. Fisher, Bonnie B. Plikaytis, Thomas M. ShinnickAn inexpensive alternative to micro arrays was developed to examine the expression of small sets of genes in parallel and used to compare direct and indirect transcript labeling methods. Psoralen-biotin direct labeling was ~10-fold less sensitive than reverse transcriptase-biotin-dUTP labeling, but the enzymatic method generated higher levels of background noise in miniarray hybridizations. The reproducibility of hybridization intensities was the … …American Journal of Biochemistry and Biotechnology, Vol. 1, Issue 1 · 31 Mar 2005 · 6,868 accessesView PDF Cite

Research ArticleOpen accessAll words, across the recordStatistical Improvement of Batch Culture with Immobilized Pichia pastoris Cells for rPOXA 1B Laccase ProductionMichelle Gouzy-Olmos, Luis M. Cháves-Tequia, María F. Rojas-Fajardo et al.Immobilized Pichia pastoris X33/pGAPZαA-LaccPost-Stop in Ca2+ alginate beads was employed for Pleurotus ostreatus rPOXA 1B laccase batch production. Sequential statistical improvement was achieved through Plackett-Burman (PBED), (PBED-T11, 29.5±0.8 UL-1), which allowed to increase activity by 2.36-fold (12.5±2.6 UL-1) obtained in a preliminary study. Following, Box-Behnken Experimental Design (BBED) was implemented and obtained enzymatic activity in PBED-T11 was further increased by … …American Journal of Biochemistry and Biotechnology, Vol. 14, Issue 2 · 26 Apr 2018 · 11,776 accessesView PDF Cite

Research ArticleOpen accessAll words, across the recordPotential of HMEC-1 Line and HUVEC Primary Culture Cells to Study the Neonatal IgG Fc Receptor in vitroLuz Belinda Ortiz-Alegría, Irma Cañedo-Solares, Felipe Vadillo-Ortega et al.The neonatal IgG Fc receptor (FcRn) plays an important role in IgG homeostasis and immunity passive transfer. Fine points regarding these mechanisms, however, are still emerging. In order to obtain information about these phenomena, it is essential to have in vitro models of endothelium that express this receptor. In this study we chose two widely used models of human endothelial … …American Journal of Immunology, Vol. 12, Issue 1 · 21 Mar 2016 · 9,446 accessesView PDF Cite

Research ArticleOpen accessAll words, across the recordSCREENING FOR CUCUMBER MOSAIC RESISTANT LINES FROM THE OVULE CULTURE DERIVED DOUBLE HAPLOID CUCUMBERSParichat Plapung, Sirisupaporn Khamsukdee, NutthaPotapohn, Prasartporn SmitamanaCMV is one of the major destructive viruses worldwide and commercial CMV resistant cucumber is very rare. Therefore we aimed to establish the ovule derived resistant lines used for the breeding program. Haploid plants of sixty-eight cucumber lines were successfully obtained by culturing the un pollinated ovaries harvested one day before an thesis on a modified MS medium supplemented with … …American Journal of Agricultural and Biological Sciences, Vol. 9, Issue 3 · 28 Feb 2014 · 7,903 accessesView PDF Cite

Research ArticleOpen accessAll words, across the recordModeling Growth of Microalgae Dunaliella Salina under Different Nutritional ConditionsSergio Santos Jesus, Rubens Maciel FilhoProblem statement: The aim of this study was to find the empirical model that describes the growth kinetics of Dunaliella salina, with low production cost and to estimate parameters of this model. Approach: In this study the strain of D. salina UTEX 200 was cultivated in seawater (0.5 M NaCl) at room temperature with agitation of 150 rpm and luminous … …American Journal of Biochemistry and Biotechnology, Vol. 6, Issue 4 · 31 Dec 2010 · 9,014 accessesView PDF Cite

Research ArticleOpen accessAll words, across the recordCallus Induction and Cellular Suspensions from Murtilla (Ugni molinae Turcz.) for trans-resveratrol ProductionSalazar Carolina, Bustos Evelyn, Perez Claudia et al.The present study reports for the first time the quantification of resveratrol and the use of biotechnological techniques applied to stilbene productions in species from genus Ugni and calli production from adult explants (fruits). Resveratrol is synthesized by a series of families of higher plants, which has generated much interest in recent years for its antioxidant, anticancer and antitumor properties, … …American Journal of Biochemistry and Biotechnology, Vol. 13, Issue 1 · 24 Jan 2017 · 7,942 accessesView PDF Cite

Research ArticleOpen accessAll words, across the recordThe Comparison of Spectrophotometric Method and High-Performance Liquid Chromatography in Photosynthetic Pigments AnalysisSabina Jodłowska, Adam LatałaProblem statement: Spectrophotometric method and High-Performance Liquid Chromatography (HPLC) are the most useful tools to photosynthetic pigments analysis. The main objective of this reaserch was to compere the results obtained by these two independent methods in experiments under controlled laboratory conditions. Approach: Comparison between spectrophotometric and HPLC methods for pigment analysis was carried out on Baltic cyanobacterium Geitlerinema amphibium, which … …OnLine Journal of Biological Sciences, Vol. 11, Issue 2 · 30 Sep 2011 · 10,865 accessesView PDF Cite