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85 articles for “Protein Yield”
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Research ArticleOpen accessIn the abstractSequence Analysis of Scaffold/Matrix Attachment Regions (S/MARs) From Human Embryonic Kidney and Chinese Hamster Ovary CellsNur Shazwani Mohd Pilus, Azrin Ahmad, Nurul Yuziana Mohd YusofBinding of intergenic Scaffold/Matrix Attachment Regions (S/MARs) to nuclear matrix proteins is believed to poise adjacent genes for transcription by forming chromatin loops. Vector constructs containing Scaffold/Matrix Attachment Regions (S/MAR) flanking the gene of interest, therefore, are able to enhance recombinant protein expression in mammalian cells. We compared two methods that are based on buffers containing 2M NaCl and Lithium-3,5-diidosalicylate … …OnLine Journal of Biological Sciences, Vol. 18, Issue 4 · 8 Oct 2018 · 7,630 accessesView PDFCite
Research ArticleOpen accessIn the abstractIn Silico Prediction and Functional Characterization of Genes Related to Abiotic and Biotic Stresses in Chickpea (Cicer arietinum)Sukhdeep Kaur, Satendra Singh, Gitanjali Tandon et al.Chickpea (Cicer arietinum L.) is second largest grown legumes worldwide contributing 75% of total pulse production. It is a cool season legume crop and grown in tropical and subtropical areas. Due to drastic climatic changes, chickpea suffers from many biotic (blight and wilt) and abiotic (salinity, drought, cold) stresses that directly impact the growth and yield. In our study, we … …Current Research in Bioinformatics, Vol. 7, Issue 1 · 25 Sep 2017 · 14,188 accessesView PDFCite
Research ArticleOpen accessIn the abstractWays of Increasing Seed Germination of Sweet Clover and Methods of Reducing the Amount of Coumarin in the Leaf-Stem MassMarden Ersainovich Baidalin, Igilik Imangalievich Zhumagulov, Ermek Ualikhanovich Sagalbekov, Ualikhan Molgazhdarovich SagalbekovThe article presents the results of laboratory and field experiments on studying the influence of ways of presowing seed treatment and methods of reducing the amount of coumarin on yield and forage quality of sweet clover in the conditions of Northern Kazakhstan. The aim of the research is to study and to prove the effective way of presowing treatment of … …OnLine Journal of Biological Sciences, Vol. 17, Issue 2 · 17 Jun 2017 · 7,241 accessesView PDFCite
Research ArticleOpen accessIn the abstractOptimization of Hydrolysis Conditions for the Production of Iron-Binding Peptides from Scad (Decapterus maruadsi) Processing ByproductsWenting Zhang, Yanan Li, Jie Zhang, Guangrong HuangThe scad (Decapterus maruadsi) processing byproduct (SPB) was hydrolyzed by four commercial enzymes, namely, trypsin, flavourzyme, protamex and alcalase, for preparing high Iron-Binding Capacity (IBC) hydrolysate. Alcalase was the best choice for obtaining high IBC hydrolysate from SPB. Response surface methodology using a central-composite design was employed to optimize the enzymatic hydrolysis conditions with alcalase to obtain a maximum hydrolysate … …American Journal of Biochemistry and Biotechnology, Vol. 12, Issue 4 · 17 Oct 2016 · 7,589 accessesView PDFCite
Research ArticleOpen accessIn the abstractQuality Herbage Production of Dwarf Napiergrass with Italian Ryegrass Cropping under Digested Effluent Application in Southern Kyushu, JapanHadijah Hasyim, Yasuyuki Ishii, Ahmad Wadi, Sachiko IdotaDigested effluent produced by a biogas-plant contains essential plant nutrients to solve the treated and disposal problems of livestock manure. Dwarf variety of late-heading type (dwarf) napiergrass as a perennial summer crop and intercropping of over-sown Italian ryegrass as an annual winter crop were applied to 3 levels of digested effluent in southern Kyushu from 2007 to 2009. Digested effluent … …American Journal of Agricultural and Biological Sciences, Vol. 11, Issue 1 · 25 Feb 2016 · 7,552 accessesView PDFCite
Research ArticleOpen accessIn the abstractAssessment of Immunogenicity of CP8, CLFA-FnBPB and CP8-CLFA-FnBPB AntigensXiuli Liu, Yongqing Hao, Shuyi Wang et al.To assess the effects of conjugating Capsular Polysaccharide (CP) with Clumping factor A (ClfA) and Fibronectin-Binding Proteins B (FnBPB) on the immunogenicity of the antigen, CP was isolated and purified from serotype 8 (CP8) Staphylococcus aureus from bovine mastitis dairy herds in Inner Mongolia and ClfA-FnBPB was produced with Escherichia coli encoding a fusion protein ClfA-FnBPB gene. The CP8-ClfA-FnBPB complete … …American Journal of Animal and Veterinary Sciences, Vol. 8, Issue 3 · 30 Aug 2013 · 7,545 accessesView PDFCite
Research ArticleOpen accessIn the abstractGenetic Polymorphism Exon 9-11 at the Leptin Gene Receptor in Breeder Hens of Mazandaran Native FowlsHossein Ali Abbasi, Shahabodin Gharavysi, Rohullah AbdullahpourProblem statement: Leptin a 16 kD protein synthesized by white adipose tissue and involved in regulation of feed intake, energy balance, fertility and immune function. In order to evaluate the leptin gene receptor polymorphism, we have used a Restriction Fragment Length Polymorphism (RFLP) method. Approach: Blood samples were collected from randomly chosen 100 native fowls breeding station of Mazandaran. The … …American Journal of Animal and Veterinary Sciences, Vol. 6, Issue 2 · 17 Sep 2011 · 7,700 accessesView PDFCite
Research ArticleOpen accessIn the abstractToxicological Response of the Green Alga Chlorella vulgaris, to Some Heavy MetalsE. Afkar, H. Ababna, A. A. FathiProblems statement: The disturbance of aquatic ecosystems provoked by heavy metals pollution from industrial and domestic sources, has as consequence the loss of biological diversity, as well as increased bioaccumulation and magnification of toxicants in the food chain. The aim of this study was to evaluate the effect of some heavy metals on some physiological activities of Chlorella vulgaris beyerinck … …American Journal of Environmental Sciences, Vol. 6, Issue 3 · 30 Jun 2010 · 10,778 accessesView PDFCite
Research ArticleOpen accessIn the abstractEffects of Rumen-Protected Methionine on Dairy Performance and Amino Acid Metabolism in Lactating CowsW.R. Yang, H. Sun, Q.Y. Wang et al.Problem statement: Free Met as one of the most limiting AA in dairy cows would be mostly degraded in the rumen. This study was to determine the effect of different levels of Rumen-Protected Met (RPMet) on dairy performance and serum amino acid metabolism. Approach: Thirty-six Holstein cows in similar condition were randomly assigned to six experimental treatments with six replicates … …American Journal of Animal and Veterinary Sciences, Vol. 5, Issue 1 · 31 Jan 2010 · 8,262 accessesView PDFCite
Research ArticleOpen accessIn the abstractPurification of Chymotrypsin-Trypsin Inhibitor from Winged Bean Seeds using Single Step Immunoaffinity ColumnSanhita Roy, Samir Kumar DuttaProblem statement: Winged bean is a rich source of protease inhibitors. The seeds of winged bean contain several serine protease inhibitors of which winged bean chymotrypsin-trypsin inhibitor (WbCTI) is of significant importance due to its dual inhibition of both chymotrypsin and trypsin. The purification of WbCTI has always been difficult and a time taking process that involved large number of … …American Journal of Biochemistry and Biotechnology, Vol. 5, Issue 3 · 30 Sep 2009 · 7,095 accessesView PDFCite