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94 results for “primer”

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Research ArticleOpen accessIn the abstractCharacterization of Mi1.2 Whitefly (Bemisia tabaci) Resistance GeneSherin Amin Mahfouze, Shipra Saxena, Heba Amin Mahfouze, Manchikatla Venkat RajamTomato (Solanum peruvianum) Mi gene provides resistance to whitefly (Bemisia tabaci), potato aphids and nematode making Mi a useful source in integrated pest management program. The aim of this work was to isolate, clone and sequence Mi1.2 gene from S. peruvianum. In addition, physico-chemical identification of amino acids deduced from Mi1.2 gene was done. Secondary (2D) and tertiary (3D) structures … …OnLine Journal of Biological Sciences, Vol. 17, Issue 4 · 15 Nov 2017 · 7,793 accessesView PDFCite
Research ArticleOpen accessIn the abstractCariogenic Pathogen Scardovia Wiggsiae Screening Among Pediatric Orthodontic Patients: A Pilot StudyWeston Milne, Ghazaleh Rezaei, Adam Whiteley, Karl KingsleyDental caries remains one of the most prevalent oral health diseases in the United States, affecting nearly half of all children and a majority of adults. Most medically important cariogenic bacteria, including Streptococcus, Lactobacillus, Actinomyces and Veillonella species are well known, although recent evidence has identified the new cariogenic pathogen Scardovia wiggsiae (S. wiggsiae) among children and minorities with severe … …Current Research in Dentistry, Vol. 9, Issue 1 · 6 Oct 2017 · 11,869 accessesView PDFCite
Research ArticleOpen accessIn the abstractReassessment of Molecular Variation in Isolated Populations of Deschampsia cespitosa from Metal Contaminated Regions in Northern Ontario (Canada) after 17 Years of Potential Genetic RecombinationSabrina Rainville, Peter Beckett, Kabwe NkongoloThe effects of ore extraction and processing procedures in the Greater Sudbury and Cobalt regions have been long-lasting. The objective of this study is to determine the current level of genetic variation in Deschampsia cespitosa populations from metal contaminated and uncontaminated sites in samples collected in Northern Ontario in 2016 after 17 years of potential genetic recombination since the last … …American Journal of Environmental Sciences, Vol. 13, Issue 4 · 13 Jul 2017 · 7,044 accessesView PDFCite
Research ArticleOpen accessIn the abstractSex Identification and the Sex-Related Genes of Nipponia NipponFeilong Sun, Xuanfu Huang, Shuiyun YangSex identification in birds is necessary for studying their ecology, behavior and population dynamics and structure. This study was carried out to determine the sex of Nipponia nippon by a Polymerase Chain Reaction (PCR) method. The sexing universal primers of non-ratite birds were employed to amplify the sexing related genes and identify the sex of each individual. A 657 bp … …American Journal of Biochemistry and Biotechnology, Vol. 13, Issue 2 · 30 May 2017 · 7,841 accessesView PDFCite
Research ArticleOpen accessIn the abstractPhylogenetic of ERIC-DNA Fingerprinting and New Sequencing of Aeromonas Species and V. Cholerae DNAHawraa Natiq Kabroot AL-Fatlawy, Hawraa Abdalameer Aldahhan, Ali Hmood AlsaadiThe current study included 44 isolate of A. hydrophila, A. sobria and V. cholerae and other bacteria isolated from stool samples and environmental samples (Kufa river water and hospital environmental samples). ERIC DNA Fingerprinting with ERIC primers pairs generated distinct amplification bands ranging in size from (87 bp to 8000 kb). The 44 isolates produced 93 different patterns by ERIC … …American Journal of Applied Sciences, Vol. 14, Issue 10 · 5 May 2017 · 7,801 accessesView PDFCite
Research ArticleOpen accessIn the abstractInfluence of Probiotics on Coccidia, H. Contortus and Markers of Infection in GoatsKwaku Barima Gyenai, Mulumebet Worku, Mehrdad Tajkarimi, Salam IbrahimImmunostimulants can induce nonspecific resistance against parasites. The use of probiotics to control development of animal gastrointestinal parasites could help reduce the risks of infestation. In this study, we investigated the effects of probiotics administration on gastrointestinal parasites coccidia, H. contortus and markers of infection. A cocktail of probiotics mix including Bifidobacterium longum, Bifidobacterium breve, Lactobacillus acidophilus, Lactobacillus reuteri and … …American Journal of Animal and Veterinary Sciences, Vol. 11, Issue 3 · 29 Jul 2016 · 9,028 accessesView PDFCite
Research ArticleOpen accessIn the abstractExtended-Spectrum β-Lactamases Producing Escherichia coli Strains Monitored Over 4 Years in The University Hospital in Košice, SlovakiaViera Lovayová, Lucia Vargová, Mária Nagyová et al.The beta-lactamases with extended spectrum of activity (ESBL) are medically one of the most important group of enzymes. The presented study provides identification and determination of the spectrum of resistance against different and clinically used antimicrobial drugs in the clinical isolates of Escherichia coli. These isolates had their origin in different departments of the University Hospital L. Pasteur in Košice. … …Current Research in Microbiology, Vol. 7, Issue 1 · 16 Jun 2016 · 14,537 accessesView PDFCite
Research ArticleOpen accessIn the abstractGenetic Relationship among Wild Medicinal Genotypes of Ziziphora canescens Benth. and Ziziphora tenuior L. and Detection of Genetic Variations Resulted From Tissue Culture, Salinity and pH MediaAbdulkarim Dakah, Mohamad Suleiman, Salim ZaidZiziphora tenuior L. and Ziziphora canescens Benth. are two of plants which using in folk medicine in the Kalamoon Mountains areas of Syria for cough, stomachache and dysentery. Samples of Ziziphora genotypes were collected from four different locations (Assal Al-Ward, Yabroud, Qarah and Maaraba), 20 RAPD and 12 ISSR primers were used to assess the genetic diversity of 10 genotypes. … …American Journal of Agricultural and Biological Sciences, Vol. 10, Issue 3 · 19 Oct 2015 · 7,897 accessesView PDFCite
Research ArticleOpen accessIn the abstractSerological and Molecular Investigation of Cutaneous Leishmaniasis in Healthy Individuals from an American Cutaneous Leishmaniasis-Endemic RegionRejane Cristina Ribas-Silva, Taisa Rocha Navasconi, Laís de Souza Braga et al.American Cutaneous Leishmaniasis (ACL) is a major, clinically relevant zoonosis in Brazil. The disease spectrum includes single, localized, cutaneous ulcers, diffuse cutaneous leishmaniasis and mucosal disease. A subclinical form of the disease has also been described in individuals living in ACL-endemic regions. The goal of this study was to employ immunological and molecular diagnostic methods to evaluate the presence of … …American Journal of Infectious Diseases, Vol. 11, Issue 1 · 23 Jun 2015 · 7,324 accessesView PDFCite
Research ArticleOpen accessIn the abstractFungal Concentrations in Dust Samples of Animal Rearing Houses of Bangalore, IndiaPavan RangahanumaiahFungal concentrations in dust samples analyzed by molecular methods were carried out of animal rearing house at Hessaraghatta village, Bangalore. Handy air sampler EnviroTech-APM 821 was used to collect the dust samples in triplicate and sampling time was limited to eight hours. The sampled filter papers were used for the assay of DNA extracted from all the four animal rearing … …Current Research in Microbiology, Vol. 6, Issue 1 · 8 Jun 2015 · 7,537 accessesView PDFCite