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Research ArticleOpen accessEvery word in the titleApplication of a Serum/Protein-Free Medium for the Growth of Mammalian Cell Lines and High Level Production of Classical, Variant and Very Virulent Strain of Infectious Bursal Disease Virus (IBDV)Ane Franciele Tapparo, Paloma Oliveira Tonietti, Maria CecÍlia R. Luvizotto, Tereza C. CardosoThe aim of this study was to verify the capacity of IBDV strains, Lukert, F52/70 and very virulent Brazilian genotype (G11) to infect and replicate on cell culture maintained without serum or protein supplementation. BHK21 and CER cells were directed adapted to growth under absence of protein and/or fetal bovine serum with addition of 5 µg mL-1 of insulin-like growth … …American Journal of Agricultural and Biological Sciences, Vol. 3, Issue 2 · 30 Jun 2008 · 7,656 accessesView PDFCite
Review ArticleOpen accessEvery word in the titleOverview of Klebsiella Pneumoniae as a Nosocomial Pathogen and ESBL Producing Strains in IranFariba Akrami, Amirmorteza Ebrahimzadeh Namvar, Iman Vazife SirzariKlebsiella pneumoniae is one of the most important human bacterial pathogens with an extensive range of community and hospital acquired infections that may lead to morbidity and mortality. Evaluating the prevalence and epidemic sources of infections and the pathogenicity mechanism of bacteria can be investigated by various typing methods. The emergence of multi-drug resistant strains and extended-spectrum ß-lactamase (ESBL) producing … …American Journal of Infectious Diseases, Vol. 15, Issue 1 · 11 Mar 2019 · 7,890 accessesView PDFCite
Research ArticleOpen accessEvery word in the titleSeparation and Identification of Two Fungal Strains in Stored MaizeXiaoyu Yue, Lynne McLandsborough, Jian Zou, Xueqin GaoThe microbiological spoilage of stored grain is important for human health and economic loss. Two major predominant grain spoilage fungi (M15 and M16) were isolated from maize from a granary in China. Morphological observation of the fungal isolates, analysis of their ITS sequences and construction of their phylogenetic trees were conducted on these two strains, which were identified as Fusarium … …American Journal of Biochemistry and Biotechnology, Vol. 13, Issue 3 · 3 Aug 2017 · 7,331 accessesView PDFCite
Research ArticleOpen accessEvery word in the titleResistance in Escherichia coli Strains Isolated from Pig Faecal Samples and Pig farm Workers, GreeceGeorge Valiakos, Alexandros Vontas, Constantina N. Tsokana et al.In this study, we compare the degree of antibiotic resistance between the E. coli strains isolated from swine faecal and farm workers. Resistance in nine widely used antibiotics was assessed by the determination of Minimum Inhibitory Concentration. Sixty-four out of 72 E. coli isolates derived from pigs and sixty out of 72 derived from farm workers showed resistance to at … …American Journal of Animal and Veterinary Sciences, Vol. 11, Issue 4 · 5 Nov 2016 · 7,505 accessesView PDFCite
Research ArticleOpen accessEvery word in the titleEvaluation of Different Drugs in Down-Regulation of Efflux Pump Genes expression in Methicillin-Resistant Staphylococcus aureus StrainsManu Chaudhary, Santosh Kumar Patnaik, Anurag PayasiAntimicrobial resistance caused by efflux systems is not widely studied in Staphylococcus aureus. In the present study, prevalence of NorA and NorB efflux pumps was studied in thirteen Methicillin-Resistant Staphylococcus aureus (MRSA) strains using a pair of primers with Polymerase Chain Reaction (PCR). Antibiotic susceptibility study in NorA and NorB efflux positive isolates was carried out according to the Clinical … …American Journal of Infectious Diseases, Vol. 10, Issue 4 · 7 Jan 2015 · 7,451 accessesView PDFCite
Research ArticleOpen accessEvery word in the titleCirculation of Multidrug-Resistant (MDR) and Extensively Drug-Resistant (XDR) Mycobacterium Tuberculosis Strains in MexicoCarlos A. Vazquez-Chacon, Armando Martinez-Guarneros, Susana Balandrano Campos et al.Emergence of Extensively Drug-Resistant (XDR) Tuberculosis (TB) is an important public health problem worldwide. In Mexico, the extent of circulation of XDR-TB strains is unknown. Here, the drug-resistance patterns of TB isolates collected in Mexico between 2006 and 2008 were analyzed. Among 137 TB isolates, (58%) were classified as MDR exclusively, (33%) were Pre-XDR and (9%) were assigned as XDR. … …American Journal of Infectious Diseases, Vol. 10, Issue 4 · 7 Jan 2015 · 7,422 accessesView PDFCite
Research ArticleOpen accessEvery word in the titleDifferential Activities of Glutathione S-Transferase Isoenzymes in Strains of Fasciola Hepatica Susceptible and Resistant to TriclabendazoleV. Fernández, P. Ortiz, M.V. Solana, H. SolanaFasciolosis, a parasitic zoonosis of intrahepatic location, is caused by the trematode Fasciola hepatica. Its control is mainly based on the use of the anthelminthic Triclabendazole (TCBZ). The indiscriminate use of this drug has favored the development of anthelmintic resistance. The Glutation S-Transferases (GSTs) are multifunctional enzymes involved in the detoxification of xenobiotics and endogenous compounds using conjugation with endogenous … …American Journal of Animal and Veterinary Sciences, Vol. 9, Issue 4 · 10 Dec 2014 · 7,574 accessesView PDFCite
Research ArticleOpen accessEvery word in the titlePhylogenetic Study of Some Strains of DunaliellaDuc Tran, Trung Vo, Sixto Portilla et al.Dunaliella strains were isolated from a key site for salt production in Vietnam (Vinh Hao, Binh Thuan province). The strains were identified based on Internal Transcribed Spacer (ITS) markers. The phylogenetic tree revealed these strains belong to the clades of Dunaliella salina and Dunaliella viridis. Results of this study confirm the ubiquitous nature of Dunaliella and suggest that strains of … …American Journal of Environmental Sciences, Vol. 9, Issue 4 · 28 Aug 2013 · 7,692 accessesView PDFCite
Research ArticleOpen accessEvery word in the titleISOLATION AND CHARACTERIZATION OF HELICOBACTER PYLORI STRAINS FROM GASTRIC BIOPSIES OF ALGERIAN PATIENTSGuetarni Hassina, Bensoltane AhmedThe objective of this study is to isolate and characterize strains Helicobacetr pylori from gastric biopsies by upper gastrointestinal endoscopy. After a rapid urease test, two Helicobacter pylori strains isolated from four patients on blood agar. After extraction of DNA from these strains from homogenates of gastric biopsies, quantification by real-time PCR was performed to determine Helicobacter pylori on the … …OnLine Journal of Biological Sciences, Vol. 13, Issue 2 · 19 Apr 2013 · 7,264 accessesView PDFCite
Research ArticleOpen accessEvery word in the titlePrevalence of Drug-Resistant Strains of Streptococcus Pneumoniae in AbakalikiIroha Ifeanyichukwu Romanus, Okafor Florence Njideka, Nwakaeze Emmanuel Amobi et al.Streptococcus pneumoniae is a major cause of illness such as pneumonia, meningitis, bacteremia and otitis media in children and the elderly. The emergence of drug-resistant strains threatens to complicate the management of these diseases. We conducted a hospital-based surveillance for drug-resistant Strep. Pneumoniae in outpatients with pneumococcal infection in Abakaliki Ebonyi State Nigeria. Between 2003-2005 from January through December, a … …American Journal of Infectious Diseases, Vol. 8, Issue 3 · 26 Nov 2012 · 7,677 accessesView PDFCite