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79 results for “Polymerase Chain Reaction”
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Research ArticleOpen accessIn the abstractThe Association of HLA Class II Alleles with the Response to Alfa-Interferon/Ribavirin Therapy in Chronic Hepatitis C PatientsAwny Aly Abol-Ez Gawish, Nahla Abd El-Hamid Mohammed, Rasha Adel Sayed Ahmed Hussein, Elsaid Galal El-BadrawyHuman Leucocyte Antigens (HLA) class II appears to play an important role in the individual’s immune response to viral infection. The present study was aimed at assessment of the relationship between HLA DRB1 alleles and the response to HCV combined therapy in Chronic Hepatitis C patients (CHC). We enrolled a total of 44 chronically infected HCV patients without Hepatitis B … …American Journal of Immunology, Vol. 11, Issue 3 · 12 Nov 2015 · 6,704 accessesView PDFCite
Research ArticleOpen accessIn the abstractAmino Acid Sequence of Amylase Type Alpha, MiAmy, from Ok-Rong Mango (Mangifera indica Linn. cv. Ok-Rong)Raksmont Ubonbal, Saijai Posoongnoen, Jureerut Daduang et al.The 594-amino acid residue sequence of α-amylase, MiAmy, from the Ok-rong mango (Mangifera indica Linn. cv. Ok-rong), in the ripening stage, was determined through Reverse Transcription Polymerase Chain Reaction (RT-PCR). Sequence alignments and evolutionary tree analyses revealed its high similarity to plastid α-amylase from many other plants. The sequence was revealed to have four conserved regions with catalytic amino acid … …American Journal of Biochemistry and Biotechnology, Vol. 11, Issue 3 · 19 Aug 2015 · 7,217 accessesView PDFCite
Research ArticleOpen accessIn the abstractExpression Pattern of the Orphan Nuclear Receptor, Nurr1, in the Developing Mouse Forelimb and its Relationship to Limb Skeletogenesis and OsteogenesisHeba A. Ahmed, Lidia L. Ibrahim, Desouki A. El Mekkawy, Abeer El WakilThe NR4A orphan nuclear receptor, Nurr1, has been shown to regulate the expression of osteoblastic genes and osteoblastic differentiation. However, the expression profile of Nurr1 in the developing mouse forelimb and its relationship to skeletogenesis has not, to the best of our knowledge, been previously analyzed. In this study, the relationship between Nurr1 expression pattern, skeletogenesis and osteogenesis in the … …OnLine Journal of Biological Sciences, Vol. 15, Issue 3 · 24 Jul 2015 · 6,952 accessesView PDFCite
Research ArticleOpen accessIn the abstractThe Comparison of RT-LAMP, RT-PCR and Dot-Blot Hybridization for Detection of Jembrana Disease VirusAsmarani Kusumawati, Tenri A. Wanahari, Issabellina D. Tampubolon, Basofi A. MappakayaJembrana disease virus is a viral pathogen that causes Jembrana disease in Bali cattle (Bos javanicus) with high mortality rate. Infection of Jembrana Disease Virus (JDV) on Bali cattle have caused substantial economic losses for farmers in Indonesia and Australia. In order to control the spread, development of a sensitive detection method is important. In this study, we used three … …American Journal of Biochemistry and Biotechnology, Vol. 11, Issue 2 · 26 Jun 2015 · 7,802 accessesView PDFCite
Research ArticleOpen accessIn the abstractThe Association of Early Pregnancy Loss with Polymorphism in Xenobiotics Detoxification GenesElena Vladimirovna Mashkina, Konstantin Nikolaevich Saraev, Elena Viktorovna Butenko et al.Pregnancy loss and other pregnancy complication can be connected with environmental and lifestyle risk factor, among which effect of chemical compounds is the strongest. Effects of xenobiotics can be modified by allele variants of xenobiotic detoxification enzymes phase I or II. A total of 71 women with early pregnancy loss and 101 control patients were examined by a case-control methodology. … …American Journal of Applied Sciences, Vol. 12, Issue 3 · 6 May 2015 · 7,036 accessesView PDFCite
Research ArticleOpen accessIn the abstractEvaluation of Different Drugs in Down-Regulation of Efflux Pump Genes expression in Methicillin-Resistant Staphylococcus aureus StrainsManu Chaudhary, Santosh Kumar Patnaik, Anurag PayasiAntimicrobial resistance caused by efflux systems is not widely studied in Staphylococcus aureus. In the present study, prevalence of NorA and NorB efflux pumps was studied in thirteen Methicillin-Resistant Staphylococcus aureus (MRSA) strains using a pair of primers with Polymerase Chain Reaction (PCR). Antibiotic susceptibility study in NorA and NorB efflux positive isolates was carried out according to the Clinical … …American Journal of Infectious Diseases, Vol. 10, Issue 4 · 7 Jan 2015 · 7,454 accessesView PDFCite
Research ArticleOpen accessIn the abstractInhibition of Panton-Valentine Leukocidin Toxin Induced Neutrophil Cell Lysis by Vancoplus in Methicillin-Resistant Staphylococcus Aureus InfectionsManu Chaudhary, Santosh Kumar Patnaik, Anurag PayasiPantone Valentine Leukocidin (PVL) is a cytotoxin associated with virulence of Methicillin-Resistant Staphylococcus aureus (MRSA). This study was undertaken to analyze the prevalence of PVL among MRSA strains and to study the effect of adjuvants (L-lysine, L-arginine, Ethylenediamine Tetraacetic Acid (EDTA) as well as drugs on PVL induced neutrophil cell lysis. Further, in vitro activity of Vancoplus, vancomycin, linezolid, teicoplanin … …American Journal of Infectious Diseases, Vol. 10, Issue 4 · 20 Dec 2014 · 7,110 accessesView PDFCite
Research ArticleOpen accessIn the abstractInhibition of Interleukin-6 Expression by Curcumin in Rat Vascular Smooth Muscle Explants In VitroYanti, Stephanie, Yuliani et al.In atherosclerotic plaques, infectious agents from microbes may release Lipopolysaccharide (LPS) and heat shock proteins that can stimulate the production of mediators, i.e., pro-inflammatory cytokines, by vascular endothelial cells and Smooth Muscle Cells (SMCs). The elevated level of Interleukin-6 (IL-6) is strongly associated with the development of atherosclerosis. Here, we investigated whether curcumin isolated from the rhizome of Curcuma longa … …American Journal of Biochemistry and Biotechnology, Vol. 10, Issue 4 · 15 Dec 2014 · 6,777 accessesView PDFCite
Research ArticleOpen accessIn the abstractHypoxia-Inducible Factor 1α Expression in Chorionic Tissue and Decidua of Women with Spontaneous Abortion at the First Trimester of PregnancyE. V. Mashkina, K. A. Kovalenko, E. V. ButenkoOxygen-regulated genes expression has important role in pre-implantation embryonic metabolism regulation. Hypoxia Inducible Factor (HIF) regulated by hypoxia oxygen tension is crucial for placenta development. But the data about its role in spontaneous abortion is very poor. Thus, we aimed to determine an expression level of HIF-1α in chorionic tissue and decidua at pregnancy. Samples of chorionic tissue and decidua … …American Journal of Biochemistry and Biotechnology, Vol. 10, Issue 4 · 18 Nov 2014 · 6,866 accessesView PDFCite
Research ArticleOpen accessIn the abstractA NEW PCR-RFLP FOR SPECIES-SPECIFIC DIAGNOSIS OF SOUTH AMERICAN ANIMAL TRYPANOSOMIASISLiliana Torcoroma García, Yezid A. Ardila, Deilis Rincón et al.The diagnosis of animal trypanosomiasis in field samples is currently based on clinical suspicion and less frequently on traditional parasitological methods characterized by their very low sensitivity, especially in the sub-acute and chronic phase of the infection. Molecular tools such as Polymerase Chain Reaction have been shown to reach the highest sensitivity percentages, but without good resolution for all of … …American Journal of Animal and Veterinary Sciences, Vol. 9, Issue 2 · 5 Jun 2014 · 7,186 accessesView PDFCite