74 articles for “Molecular Characterization”

Research ArticleOpen accessIn the abstractCharacteristics of a Novel Highly Thermostable and Extremely Thermophilic Alkalitolerant Amylase from Hyperthermophilic Bacillus Strain HUTBS71Farouk Al-Quadan, Hazem Akel, Rasha NatshiProblem statement: This study reported the purification and characterization of a novel highly thermostable alkaline amylase from a newly isolated Bacillus strain HUTBS71. Approach: The enzyme was purified using ammonium sulfate precipitation, ion exchange and gel filtration chromatography. Results: Maximum amylase activity (72 U mL-1) was obtained at 100°C after 10 min of incubation. The enzyme was purified 24 fold … …OnLine Journal of Biological Sciences, Vol. 9, Issue 3 · 30 Sep 2009 · 7,400 accessesView PDF Cite

Research ArticleOpen accessIn the abstractDiscrimination Capacity of RAPD, ISSR and SSR Markers and of their Effectiveness in Establishing Genetic Relationship and Diversity among Egyptian and Saudi Wheat CultivarsSalah El-Din El-Assal, Ahmed GaberProblem statement: Yield crop cultivars and landraces are valuable sources of genetic variations that the knowledge and implication of these variations are critical in the plant breeding programs. our major objective of this study is investigating the discriminating capacity of RAPD, ISSR and SSR markers and of their effectiveness in establishing genetic relationship and diversity among Egyptian and Saudi wheat … …American Journal of Applied Sciences, Vol. 9, Issue 5 · 9 Mar 2012 · 7,768 accessesView PDF Cite

Research ArticleOpen accessIn the abstractDrug-Protein Interactions for Clinical Research by Nucleic Acid Programmable Protein Arrays-Quartz Crystal Microbalance with Dissipation Factor Monitoring Nanoconductometric AssayClaudio Nicolini, Rosanna Spera, Nicola Luigi Bragazzi, Eugenia PechkovaConductometric monitoring of drug-gene and drug-protein interactions is of fundamental importance in the field of molecular pharmacology. Here, we present our main findings and characterizations of an important antiblastic used in neuro-oncology (Temozolomide), interacting with selected proteins that represent predictive biomarkers of the rate survival of the patients, of the outcome of chemotherapy and resistance to drug itself (namely, BRIP1 … …American Journal of Biochemistry and Biotechnology, Vol. 10, Issue 3 · 17 Nov 2014 · 8,506 accessesView PDF Cite

Research ArticleOpen accessAll words, across the recordCharacterization of Fructan Content from Stem Juices of Different Varieties of Agave Atrovirens, Traditionally Used for the Production of Pulque. Production of a Sweetening Syrup by Chemical and Enzymatic ProcessingKaren Gott-Bravo, Arnulfo Pluma-Pluma, Victor Eric López-López et al.Agave atrovirens, the hardiest species of Agave in Mexico, thrives in tough, infertile soils and extreme temperatures across a wide range of altitudes, particularly in impoverished rural areas of Central and Northern Mexico. Its potential for agroindustrial use could offer a pathway to social and economic prosperity for many communities. Traditionally, A. atrovirens is tapped for its sap, known as … …American Journal of Agricultural and Biological Sciences, Vol. 19, Issue 1 · 4 Mar 2025 · 7,670 accessesView PDF Cite

Research ArticleOpen accessAll words, across the recordPreparation, Characterization and Antioxidant Activity of a Novel Acetylated Polysaccharide from Flammulina VelutipesYingyun Peng, Han Jiang, Yufeng Wu et al.In this study, a novel polysaccharide FVP2a wasextracted and purified from F. velutipes. Ac-FVP2a was prepared bychemical acetylation. The preparation process of Ac-FVP2a was optimized usingthe response surface method based on three experimental factors includingreaction time, reaction temperature, and the ratio of FVP2a to aceticanhydride. The physicochemical characteristics andantioxidant activities of Ac-FVP2a in vitro were investigated. The results showed that … …American Journal of Biochemistry and Biotechnology, Vol. 18, Issue 3 · 20 Sep 2022 · 5,207 accessesView PDF Cite

Research ArticleOpen accessAll words, across the recordUltrasound-Assisted Enzymatic Extraction of Polysaccharides from Waste Corn Bract: Process Optimization, Characterization, Antioxidant and Anti-Diabetic PotentialsYihui Liu, Yayi Li, Na Niu et al.Corn bract is the main agricultural waste of corn production and processing. Little information is focused on its value-added utilization. In the present study, the Ultrasound-Assisted Enzymatic Extraction (UAEE) was used for the first time to extract Corn Bract Polysaccharides (CBPs). The optimum conditions of UAEE for CBPs extraction were: Cellulase amount of 0.7%, enzymolysis for 58 min, and ultrasound … …American Journal of Biochemistry and Biotechnology, Vol. 18, Issue 2 · 28 Jun 2022 · 6,126 accessesView PDF Cite

Research ArticleOpen accessAll words, across the recordHeterologous Expression and Biochemical Characterization of Lipase from Burkholderia cepacia Lu10-1Yao Zhang, Lu Wang, Yuanda SongEven though lipase genes from a variety of microorganism have been cloned and over-expressed, the prospective lipase resources for commercial production and industry application are still limited. In the present study, a lipase from Burkholderia cepacia Lu10-1 is heterologously over-expressed in Escherichia coli strain BL21(DE3) and purified to homogeneity. The molecular weight of the recombinant lipase from B. cepacia Lu10-1 … …American Journal of Biochemistry and Biotechnology, Vol. 13, Issue 4 · 23 Dec 2017 · 8,765 accessesView PDF Cite

Research ArticleOpen accessAll words, across the recordIn Silico Prediction and Functional Characterization of Genes Related to Abiotic and Biotic Stresses in Chickpea (Cicer arietinum)Sukhdeep Kaur, Satendra Singh, Gitanjali Tandon et al.Chickpea (Cicer arietinum L.) is second largest grown legumes worldwide contributing 75% of total pulse production. It is a cool season legume crop and grown in tropical and subtropical areas. Due to drastic climatic changes, chickpea suffers from many biotic (blight and wilt) and abiotic (salinity, drought, cold) stresses that directly impact the growth and yield. In our study, we … …Current Research in Bioinformatics, Vol. 7, Issue 1 · 25 Sep 2017 · 14,188 accessesView PDF Cite

Research ArticleOpen accessAll words, across the recordCharacterization of the Genetic Diversity of Peach Cultivars in Taif by RAPD-PCRMohamed A. Nagaty, Salah El-Din El-Assal, Mahmoud M. RifaatProblem statement: Peach genotypes from the Kingdom of Saudi Arabia were not adequately characterized at the biological and molecular levels. This study was carried out to characterize nine peach cultivars growing in Taif (KSA) at the biological and molecular levels. Approach: For the nine peach cultivars studied, flowering and fruiting indices were determined and eight, 10-base primers were used to … …American Journal of Applied Sciences, Vol. 8, Issue 7 · 24 Jun 2011 · 7,271 accessesView PDF Cite

Research ArticleOpen accessAll words, across the recordA Putative Serine protease from Larval Midgut of Red Palm Weevil Rhynchophorus ferrugineus (Olivier) (Coleoptera: Curculionidae): Partial Purification and Biochemical CharacterizationAhmed Al Jabr, Mahmoud Abo-El-SaadA putative serine protease was isolated and partially purified from larval midgut of red palm weevil (RPW), Rhynchophorus ferrugineus (Olivier) using anion exchange chromatography, DE-52. Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) of this protease showed that the major band has a molecular weight of approximately 24 kDa corresponding to the same molecular weight of mammalian trypsin. In addition, zymography … …American Journal of Environmental Sciences, Vol. 4, Issue 6 · 31 Dec 2008 · 7,018 accessesView PDF Cite