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82 results for “In vitro Method”
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Research ArticleOpen accessIn the abstractFlavonoids from the Zizania latifolia Bracts: Extraction Optimization, Antioxidant Activity and Exploration in Strawberry PreservationTong Wang, Yuzhu Zhang, Lijie Ben et al.Zizania latifolia bracts are Z. latifolia-based agricultural waste materials, distributed in various parts of the world. In the current study, the Response Surface Method (RSM) was used to optimize the cellulase-assisted extraction of Total Flavonoids from Z. latifolia bracts (TFZB). Then, the in vitro antioxidant capacity and the potential effect on strawberry preservation were assessed. The results showed that the … …American Journal of Biochemistry and Biotechnology, Vol. 17, Issue 1 · 15 Mar 2021 · 6,363 accessesView PDFCite
Research ArticleOpen accessIn the abstractAcetylation Process and Antioxidant Activity of a Polysaccharide from Loquat (Eriobotrya japonica) LeavesYiting Xue, Xiao Ding, Ze Zhang et al.Chemical modifications of polysaccharides can provide an opportunity to obtain new agents with possible therapeutic uses. In this study, a water-soluble polysaccharide extracted from Loquat Leaves (coded LLPs) was purified by the column chromatography, which was used to investigate its acetylation process by the acetic anhydride method. The Degree of Substitution (DS) was used as the index, the effect of … …American Journal of Biochemistry and Biotechnology, Vol. 16, Issue 4 · 1 Jan 2021 · 7,056 accessesView PDFCite
Research ArticleOpen accessIn the abstractOptimization of Chelating Process of Polysaccharides of Lyophyllum decastes with Zinc and Its Antioxidant ActivityBing Xu, Yi Dai, Jing Hong, Yiyong ChenIn this study, polysaccharides of Lyophyllum decastes (LDS) were used as raw material. The chelating rate was set as the index, the effect of chelating time, mass ratio and initial mass concentration on chelating rate was investigated. Response surface method was used to optimize the preparation technology of LDS and Zinc (Ⅱ) chelate (LDS-Zn) based on single factor experiments. LDS-Zn … …Current Research in Bioinformatics, Vol. 9, Issue 1 · 26 Aug 2020 · 11,761 accessesView PDFCite
Research ArticleOpen accessIn the abstractPhytochemical Profiles and Anti-Diabetic Potential of the Extract of Pueraria lobata Compound Teabags: Preparation and Optimization Infusion ConditionsQing Liu, Wenyue Shi, Futing Xue et al.A Pueraria lobata root compound teabag using Pueraria lobate root, Stigma maydis, and Prunus mume as the main material was prepared in this study, and the brewing technology was optimized using Response Surface Methodology (RSM). The sensory evaluation, total polysaccharide, and phenolic compound content were used as evaluation indicators and the effect of the compound ratio of raw material was … …American Journal of Biochemistry and Biotechnology, Vol. 18, Issue 4 · 26 Nov 2022 · 5,641 accessesView PDFCite
Research ArticleOpen accessIn the abstractPreparation of Low-Sugar Herbal Buccal Tablet and its Antioxidant ActivityYuqing Wang, Mingjie Sun, Rui Xu et al.In this study, in order to prepare a low-sugar Herbal Buccal Tablet (HBT), Response Surface Methodology (RSM) was used to optimize the formula of HBT. HBT was prepared by wet granulation and tableting process on the basis of formula optimization. The in vitro antioxidant activity, glycemic index and particle microstructure of HBT were evaluated. The results showed that the optimal … …American Journal of Biochemistry and Biotechnology, Vol. 18, Issue 1 · 8 Mar 2022 · 6,357 accessesView PDFCite
Research ArticleOpen accessIn the abstractExtraction, Partial Identification and Bioactivities of Total Flavonoids from Carex meyeriana KunthHongbiao Wang, Penghui Wang, Xiudong Yang et al.To date, knowledge associated with extraction, chemical constituents and bioactivities of Total Flavonoids from Carex meyeriana Kunth (TFCMK) that remains unclear. Therefore, this paper was designed to optimize the ethanol reflux extraction method by response surface methodology and determine the chemical constituents by Liquid Phase-Mass Spectrometry (HPLC-MS); additionally, the bioactivities including antioxidant, antibacterial and hemolysis test were also explored. The … …American Journal of Biochemistry and Biotechnology, Vol. 15, Issue 3 · 7 Aug 2019 · 7,523 accessesView PDFCite
Research ArticleOpen accessIn the abstractDevelopment and Optimization of Plant Extract Loaded Nanoemulsion Mixtures for the Treatment of Inflammatory DisorderMukesh Gohel, Tejal Soni, Lal Hingorani et al.In current study, a nanoemulsion as drug delivery system (NE) of spray dried Boswellia Serrata Extract (BSE) was developed and characterized to compare it in vitro/Ex vivo diffusion with reference formulation. Pseudo-ternary phase diagrams were constructed by phase diagram by aqueous titration method, of which the evaluation method was improved for solubility, Droplet size and transmittance. The formulation of BSE-loaded … …Current Research in Drug Discovery, Vol. 1, Issue 2 · 26 Jan 2015 · 14,064 accessesView PDFCite
Research ArticleOpen accessIn the abstractEMERGING METRONIDAZOLE RESISTANCE IN ANAEROBES AND MAPPING THEIR SUSCEPTIBILITY BEHAVIOURManu Chaudhary, Anurag PayasiIn the present study, anaerobic clinical isolates of Bacteroides fragilis, Escherichia coli, Staphylococcus aureus and Yersinia enterocolitica were obtained from different clinical specimens and were subjected to molecular typing to detect the gene encoding metronidazole resistance in these isolates. Subsequently, antibacterial activity of drugs was tested against the selected clinical isolates. A total of 53 clinical isolates involving 18 obligate … …American Journal of Infectious Diseases, Vol. 10, Issue 2 · 12 Apr 2014 · 9,192 accessesView PDFCite
Research ArticleOpen accessIn the abstractDihydrobiopterin (BH2): Key Determinant in Influencing Arginine Mediated Endothelial Tolerance and DysfunctionSrinidi Mohan, Harsh Patel, Jorge Bolinaga et al.The redox-sensitive tetrahydrobiopterin (BH4) is an essential cofactor that is required by endothelial Nitric Oxide Synthase (eNOS) for L-arginine (ARG) mediated Nitric Oxide (NO) generation. Oxidation of BH4 causes cofactor insufficiency and uncoupling of eNOS, resulting in product switching from NO to O2⋅– production. Here we tested the hypothesis that eNOS uncoupling is not simply a consequence of BH4 insufficiency, … …American Journal of Biochemistry and Biotechnology, Vol. 8, Issue 2 · 22 Jun 2012 · 8,348 accessesView PDFCite
Research ArticleOpen accessIn the abstractQuercetin Liposomes via Nasal Administration Reduce Anxiety and Depression-Like Behaviors and Enhance Cognitive Performances in RatsTerdthai Tong-Un, Panakaporn Wannanon, Jintanaporn Wattanathorn, Wathita PhachonpaiProblem statement: At present, the prevalence of neuropsychological disorders particularly anxiety, depression and dementia are increase. Many strategies have been developed in order to increase the efficiency of treatment and decrease side effects. Oxidative stress has been reported to play an important role on various path physiological states. Quercetin is a flavonoid commonly found in fruits and vegetables. It has … …American Journal of Pharmacology and Toxicology, Vol. 5, Issue 2 · 30 Jun 2010 · 9,034 accessesView PDFCite