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419 results for “Enzyme”
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Research ArticleOpen accessEvery word in the titlePlants Responses to Nutrients Follow the Saturation Kinetic Typical of Enzyme Systems: Biological, Economical and Environmental ImplicationsR.P. LanaAgricultural efficiency has been associated with high plant productivity. However, it is dependent on fertilizers, which are nonrenewable resources. Crop production response to fertilizers is hyperbolic, following the Michaelis-Menten model and the law of diminishing return. Data of crop production (barley, bean, corn, cotton, soybean and wheat) as a function of nitrogen, phosphorus, and potassium fertilization were analyzed by Lineweaver-Burk … …OnLine Journal of Biological Sciences, Vol. 8, Issue 1 · 31 Mar 2008 · 6,895 accessesView PDFCite
Review ArticleOpen accessEvery word in the titleA Review: Biodegradation and Applications of Keratin Degrading Microorganisms and Keratinolytic Enzymes, Focusing on Thermophiles and Thermostable Serine ProteasesWirojne Kanoksilapatham, Weeranut IntagunKeratins are hard-degrading fibrous proteins, insoluble in water and organic solvents, often accumulated in nature and major components in feathers, skins, hair, horn, nail, hoof etc., Keratin-degrading microorganisms such as bacteria, archaea, actinomycetes and fungi employ keratinases to attack keratin. Keratinases belonging to subtilisin-like serine proteases were classified based on similarity of amino acid sequences. Keratinolytic thermophilic or hyperthermophilic bacteria … …American Journal of Applied Sciences, Vol. 14, Issue 11 · 10 Nov 2017 · 9,981 accessesView PDFCite
Research ArticleOpen accessIn the keywordsPurification, Structural Characterization and Anti-Aging and Stress Activities of Cordyceps Mycelium Polysaccharides from Paecilomyces HepialiXiaokang Hu, Huixiu Ma, Guicai Chen et al.This study identified and characterized the bioactive polysaccharide components from Cordyceps mycelium powder (CMP) derived from Paecilomyces hepiali, evaluating their anti-aging and stress-protective activities. Initial screening using Drosophila melanogaster revealed that crude Cordyceps polysaccharides (CCP) exhibited comparable effects to CMP and Cordyceps aqueous extracts (CAE) on natural lifespan and UV stress resistance, suggesting CCP as the primary bioactive component. CCP … …American Journal of Biochemistry and Biotechnology, Vol. 21, Issue 4 · 16 Feb 2026 · 1,698 accessesView PDFCite
Research ArticleOpen accessIn the keywordsPotentiometric Biosensor for Highly Toxic Pb (lead) and Cd (cadmium) Detection in Milk Using Layer-by-Layer ImmobilizationZhainagul Kakimova, Klara Zharykbasova, Aitbek Kakimov et al.Milk contamination with heavy metals such as lead (Pb) and Cadmium (Cd) poses a serious threat to public health, particularly in industrial regions. To address the need for rapid and on-site detection of these contaminants, a potentiometric biosensor was developed for the quantification of Pb²⁺ and Cd²⁺ ions in milk and dairy products. The biosensor functions by measuring the change … …OnLine Journal of Biological Sciences, Vol. 25, Issue 4 · 8 Feb 2026 · 1,480 accessesView PDFCite
Research ArticleOpen accessIn the keywordsThe Effect of NaCl on the Stability of Ab3 Lipase from Sphingobacterium sp. that Co-Expressed with LEA K Using In silico ApproachIbrahim Muhammad et al.This study explores how sodium chloride (NaCl) influences the binding affinity and stability of the Ab3 lipase-LEA K complex when interacting with olive oil, using molecular docking techniques. The docking simulations, conducted with AutoDock Vina, reveal that the presence of NaCl significantly enhances the binding affinity of the enzyme-substrate complex, increasing it from-6.55 kcal/mol in the absence of salt to-7.81 … …American Journal of Biochemistry and Biotechnology, Vol. 21, Issue 1 · 9 Mar 2025 · 5,599 accessesView PDFCite
Research ArticleOpen accessIn the keywordsEffects of Soil Storage at Freezing Temperatures on Soil Enzymatic ActivitiesAinsley Lougheed, Kabwe NkongoloSoil enzyme activities are good indicators of soil health. It has been hypothesized that soil storage before analysis might affect microbial function. The objective of the present study was to determine if soil storage at -20 and -80°C affects soil enzymatic activities. Soil samples were collected and stored for four weeks at -20 and -80°C. Activities of nine enzymes were … …American Journal of Biochemistry and Biotechnology, Vol. 20, Issue 3 · 12 Aug 2024 · 4,984 accessesView PDFCite
Research ArticleOpen accessIn the keywordsDiscovery of a New Thermostable Chitinase from Streptomyces No. 6Xiaowen Que, Yonghong Bao, Qing Liu et al.Chitinases are a class of hydrolases that hydrolyze the β-1, 4-glycosidic bonds between N-acetyl-D-glucosamine (GlcNAc) in chitin. Among chitinases, thermophilic chitinases and thermostable chitinases have received extensive attention in recent years due to their ability to tolerate higher temperatures and maintain enzyme stability for a longer period of time. However, it has previously been observed that not all chitinases are … …American Journal of Biochemistry and Biotechnology, Vol. 20, Issue 3 · 1 Aug 2024 · 5,115 accessesView PDFCite
Research ArticleOpen accessIn the keywordsA Cross-Sectional Study of the Prevalence of Hepatitis C Virus InfectionOmar Atrooz, Rehab Thunibat, Ihssan AtroozLiver pain is increased by hepatotropic viruses, such as the Hepatitis C Virus (HCV). This illness can progress to cirrhosis and hepatocellular cancer. This study aims to determine the prevalence of hepatitis C in the Southern Jordanian cities (Karak and Tafilah) as well as the correlations between alanine Aminotransferase (ALT), Aspartate aminotransferase (AST), age and gender in hepatitis C patients. … …OnLine Journal of Biological Sciences, Vol. 24, Issue 4 · 5 Jul 2024 · 4,414 accessesView PDFCite
Research ArticleOpen accessIn the keywordsOptimization and Molecular Identification of Protease-Producing Thermophilic Bacterial Isolate TUA-26Anthoni Agustien, Mufidhatul Muqarramah, Feskaharny AlamsjahProtease enzymes are used as biocatalysts that can accelerate a reaction. Thermostable enzymes produced by thermophilic bacteria are needed in the industrial field because of their ability to withstand high temperatures. The thermophilic bacterial isolate TUA-26 that produces protease was to analyze the optimum conditions of temperature, pH, agitation, trace elements, carbon sources, and nitrogen sources; the stability of protease … …OnLine Journal of Biological Sciences, Vol. 24, Issue 3 · 6 Apr 2024 · 4,935 accessesView PDFCite
Research ArticleOpen accessIn the keywordsThe Enzymatic Role of Lipid Metabolism in Yarrowia lipolytica Grown on GlycerolRuixue Wang, Feifei Xin, Yufei Chang et al.More attention has been paid to the production of commercial fatty acids by microbial fermentation. The basic biochemistry of lipid accumulation in oleaginous microorganisms under nitrogen limitation has been extensively studied. As an oleaginous microorganism, Yarrowia lipolytica can produce fatty acid-derived biofuels and biochemicals. However, the response of the enzymes in Y. lipolytica to glycerol remains unclear. Therefore, we aimed … …American Journal of Biochemistry and Biotechnology, Vol. 19, Issue 2 · 17 Apr 2023 · 6,309 accessesView PDFCite