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94 articles for “primer”
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Research ArticleOpen accessIn the abstractGenetic Variation and Relationship Between Turkish Flint Maize Landraces by RAPD MarkersA. OkumusA comparative characterization of 17 flint maize landraces (Zea mays L.) was carried out using RAPD markers. Fourteen primers giving reliable and consistent polymorphic bands amplified 125 fragments (89%) with an average of 8.90 fragment per primer. Genetic variation in 17 maize landraces was characterized based on dissimilarity matrix by UPGMA dendogram which has no association into distinct grouping with … …American Journal of Agricultural and Biological Sciences, Vol. 2, Issue 2 · 30 Jun 2007 · 7,982 accessesView PDFCite
Research ArticleOpen accessIn the abstractMolecular Characterization of Black Bengal and Jamuna Pari Goat Breeds By RAPD MarkersM.A. Rahman, S.M.M. Rahman, M.A. Jalil et al.This experiment was conducted in the Genetics, Breeding and Reproductive Biotechnology Laboratory under Goat and Sheep Production Research Division, Bangladesh Livestock Research Institute (BLRI), Saver, Dhaka, Bangladesh. DNA was extracted from 14 goat breeds and the extracted DNA was observed by gel electrophoresis. Eight goat specific primers were synthesized by ASM-800 DNA synthesizer and screened in the study and all … …American Journal of Animal and Veterinary Sciences, Vol. 1, Issue 2 · 31 Dec 2006 · 9,588 accessesView PDFCite
Research ArticleOpen accessIn the abstractMolecular Characterization and in Silico Analysis of Phytochemical Compounds from Plants with Immunomodulatory PotentialHermin Pancasakti Kusumaningrum, Rejeki Siti Ferniah, Siti Nur Jannah et al.The immune system works to protect the body against foreign antigens from microorganisms and abnormal cells. The use of herbal plants as immunomodulators are used to prevent disease attacks. TNFα and interleukin-6 are pro-inflammatory immune systems that cause disease in uncontrolled synthesis. Herbal plants as inhibitors of TNFα and interleukin-6 need to be identified molecularly to reduce the occurrence of … …OnLine Journal of Biological Sciences, Vol. 25, Issue 3 · 27 Nov 2025 · 1,959 accessesView PDFCite
Research ArticleOpen accessIn the abstractGenetic Diversity of Leifsonia xyli Subsp. xyli Causing Ratoon Stunting Diseases of Sugarcane in Indonesia Using Intergenic Spacer Region Sequence AnalysisSisca Prayudani Usman, Abdul Latief Abadi et al.Leifsonia xyli subsp xyli (Lxx) is the cause of Ratoon Stunting Disease (RSD) on sugarcane and it considers as a quarantine pest in certain regions of Indonesia. RSD is a systemic disease that causes severe cane yield loss. Information about genetic diversity of this bacterial pathogen in Indonesia is limited. While global trading of sugar cane seed may facilitate the … …OnLine Journal of Biological Sciences, Vol. 25, Issue 2 · 23 Jun 2025 · 3,358 accessesView PDFCite
Review ArticleOpen accessIn the abstractSalt Tolerance and Cultivation Challenges of Jojoba (Simmondsia chinensis): A ReviewHeba Allah Adel MohassebJojoba is considered to be salt tolerant, but little is known about the extent of its tolerance and the relative influence on ion uptake and ethylene production and other factors are affecting the mode of actions of jojoba salt tolerant. According to many research has done before including our article about the glyoxalase I gene from jojoba which exhibits moderate … …OnLine Journal of Biological Sciences, Vol. 25, Issue 2 · 12 Jun 2025 · 3,323 accessesView PDFCite
Research ArticleOpen accessIn the abstractMolecular Characterization of Contagious Pustular Dermatitis Virus in Goats of Sri LankaSusanthaa Piratheepan, Sumathy Puvanendran, Kalyani PereraContagious Pustular Dermatitis (CPD), is a zoonotic disease of small ruminants causing severe deterioration of skin. Contagious Pustular Dermatitis Virus (CPDV) is an epitheliotrophic virus belonging to the parapox virus. Accurate information on CPD in Sri Lanka is scarce. This study was conducted to investigate the prevalence and to characterize the CPD virus from goats in Sri Lanka and select … …American Journal of Animal and Veterinary Sciences, Vol. 18, Issue 4 · 29 Nov 2023 · 5,349 accessesView PDFCite
Research ArticleOpen accessIn the abstractAlcohol Intubation and Synergistic Effect of Folic Acid and Egg Yolk Powder Solution on Alcohol Dehydrogenase [ADH] Class I Gene Expression and Single Nucleotide Polymorphism Frequency in Parent and Prenatal Alcohol Exposed Wistar RatsAdejoke Olukayode Obajuluwa, Oluyinka Ajibola Iyiola, Joseph Akintade Morakinyo et al.Point mutation detections in alcohol metabolizing genes could unravel molecular targets of gestational alcohol and epigenetic remodeling capacity of maternal nutrition, which underscores growth and health endpoints in Pre­natal Alcohol Exposed (PAE) fetuses. gene expression was assayed using sequenced target and internal control gene primers in a two-step thermal cycling process from isolated RNA in purely bred alcohol male and … …OnLine Journal of Biological Sciences, Vol. 24, Issue 1 · 22 Nov 2023 · 4,859 accessesView PDFCite
Research ArticleOpen accessIn the abstractWild Allium longicuspis Regel is a Feral Form of Allium sativum L. in Kazakhstan: A Comparative Molecular Genetic AnalysisAkerke Tokenova, Gulnar Sitpayeva et al.The genus Allium L comprises over 1000 species with important nutritional, medicinal and horticultural applications. The natural flora of Kazakhstan is rich in Allium species, including the wild long-pointed onion, A. longicuspis and cultivated A. sativum garlic varieties. It has been hypothesized that A. longicupis is ancestral to A. sativum. We investigated genetic polymorphism in samples of A. longicupis and … …OnLine Journal of Biological Sciences, Vol. 23, Issue 1 · 26 Dec 2022 · 6,496 accessesView PDFCite
Research ArticleOpen accessIn the abstractGenetic State of Pulsatilla Patens (L.) Mill. Coenopopulations Based on DNA LabelingRustem Saltanovich Beishov, Gulnar Sultangazina, Indira Saltanovna Beishova et al.In the article, the results of studying the gene pools of the Kazakhstan coenopopulations of a rare plant species Pulsatilla patens (L.) Mill. are shown. The biomaterial taken from five coenopopulations located in Northern Kazakhstan has been used as samples. To identify the multilocus DNA polymorphism, the authors have used ISSR (Inter Simple Sequence Repeats, or intermicrosatellite analysis) labeling. The … …OnLine Journal of Biological Sciences, Vol. 21, Issue 1 · 2 Apr 2021 · 5,946 accessesView PDFCite
Research ArticleOpen accessIn the abstractA Meat-Derived Lactic Acid Bacteria, Lactobacillus plantarum IIA, Expresses a Functional Parvulin-Like Protein with Unique Structural PropertyCahyo Budiman, Irma Isnafia Arief, Fernandes Opook, Muhammad YusufThe genome sequence of a Lactic Acid Bacterium (LAB) Lactobacillus plantarum IIA contains a single gene encoding a parvulin-like protein (Par-LpIIA). This protein belongs to Peptidyl Prolyl cis-trans Isomerase (PPIase) family proteins that catalyze a slow cis-trans isomerization of cis prolyl bond during protein folding. This study aims to provide molecular and biochemical evidences of the existence of Par-LpIIA in … …OnLine Journal of Biological Sciences, Vol. 21, Issue 1 · 26 Feb 2021 · 7,025 accessesView PDFCite