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291 results for “Z-DNA”

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Research ArticleOpen accessEvery word in the titleInteraction of a C-terminal Truncated Hepatitis C Virus Core Protein with Plasmid DNA Vaccine Leads to in vitro Assembly of Heterogeneous Virus-like ParticlesNelson Acosta-Rivero, Joanna Poutou, Alexis Mussachio et al.Recently, it has been shown that HCV core proteins (HCcAg) with C-terminal deletions assemble in vitro into virus-like particles (VLPs) in the presence of structured RNA molecules. Results presented in this work showed that a truncated HCcAg variant covering the first 120 aa (HCcAg.120) with a 32 aa N-terminal fusion peptide (6xHistag-XpressTMepitope) interacts with plasmid DNA vaccine. Interestingly, the buoyant … …American Journal of Infectious Diseases, Vol. 1, Issue 1 · 31 Mar 2005 · 6,756 accessesView PDFCite
Research ArticleOpen accessEvery word in the titleRandom Amplified Polymorphic Dna-PCR Typing of Vibrio parahaemolyticus Isolated from Local Cockles (Anadara granosa)Lesley Maurice Bilung, Son Radu, Abdul Rani Bahaman et al.Randomly amplified polymorphic DNA (RAPD) was used in this study to examine the genetic relatedness among the Vibrio parahaemolyticus strains. In the analysis by RAPD-PCR, the size for RAPD fragments ranged from 0.25 to 10.0 kb with average number of ten bands. The RAPD profiles revealed a high level of DNA sequence diversity within the Vibrio parahaemolyticus strains tested. Hence, … …American Journal of Immunology, Vol. 1, Issue 1 · 31 Mar 2005 · 8,611 accessesView PDFCite
Research ArticleOpen accessEvery word in the titleContrasting Effects of Metal Contaminations and Soil Liming on Cations Exchange Capacity and Global DNA Methylation in Betula papyrifera Populations from a Mining RegionGabriel Theriault, Kabwe NkongoloThe Greater Sudbury Region in Northern Ontario (Canada) has been one of the most contaminated regions in the world. Soil liming with dolomitic limestone applications has decreased significantly the level of soil acidity resulting in forest regeneration. This reclamation process does not affect the level of soil metal contamination but results in metals availability decrement. The coping mechanisms of birch … …American Journal of Environmental Sciences, Vol. 12, Issue 2 · 11 Apr 2016 · 7,539 accessesView PDFCite
Research ArticleOpen accessEvery word in the titleVariability of Chloroplast DNA of Extranuclear Sunflower MutantsMarkin Nicolay, Aieksandr Usatov, Maria Logacheva et al.A comparative analysis of full-genome sequences of chloroplast DNA (cpDNA) of the original inbred line 3629 and three extranuclear mutants, which were obtained by the method of mutagenesis induced with N-nitroso-N-methylurea (NMU) and characterized by different level of chlorophyll insufficiency (en:chlorina-7-yellow-green leaves; chlorophyll content (a + b)-67.8% with respect to the line 3629, variegated-10-leaves with white zones; chlorophyll content (a … …American Journal of Biochemistry and Biotechnology, Vol. 12, Issue 1 · 26 Mar 2016 · 7,272 accessesView PDFCite
Research ArticleOpen accessEvery word in the titleBIS-CATTLE: A Web Server for Breed Identification using Microsatellite DNA MarkersSarika Jaiswal, Sandeep Kumar Dhanda, M. A. Iquebal et al.Domestic cow, Bos taurus is one of the important species selected by humans for various traits, viz. milk yield, meat quality, draft ability, resistance to disease and pests and social and religious reasons. Since cattle domestication from Neolithic (8,000-10,000 years ago) today the population has reached 1.5 billion and further it’s likely to be 2.6 billion by 2050. High magnitude … …Current Research in Bioinformatics, Vol. 5, Issue 1 · 23 Jan 2016 · 9,317 accessesView PDFCite
Research ArticleOpen accessEvery word in the titleAssessment of Genetic Purity of Parental Lines of Hybrid Rice Using DNA-Based MarkersSunil Bhavsar, Twinkle Solanki, Suchita Amin, Neeru JainAssessment of hybrid seed genetic purity is one of the most important quality control parameters in hybrid seed production. With the objective of replacing the GOT with DNA based assays, Cytoplasmic Male Sterile (CMS), restorer and hybrid lines have been screened by means of microsatellite and Sequence Tagged Site (STS) markers. Cytoplasmic Male Sterile (CMS) lines often get contaminated with … …OnLine Journal of Biological Sciences, Vol. 15, Issue 2 · 13 Jun 2015 · 7,442 accessesView PDFCite
Research ArticleOpen accessEvery word in the titleDNA-MARKERS OF SUNFLOWER RESISTANCE TO THE DOWNY MILDEW (PLASMOPARA HALSTEDII)A. V. Usatov, A. I. Klimenko, K. V. Azarin et al.One of the areas of biotechnology sunflower is the development and testing of DNA markers of important agronomic traits and in particular markers of resistance to downy mildew. Resistance of 16 Rf-lines of sunflower to the races 330 and 710 of Plasmopara halstedii has been studied. Genotyping of these lines was carried out using 9 STS-markers of three Pl-loci, Pl5, … …American Journal of Biochemistry and Biotechnology, Vol. 10, Issue 2 · 24 Jul 2014 · 7,427 accessesView PDFCite
Research ArticleOpen accessEvery word in the titleIn-Vivo Evaluation of Hexavalent Chromium Induced DNA Damage by Alkaline Comet Assay and Oxidative Stress in Catla catlaKantha Deivi Arunachalam, Sathesh Kumar Annamalai, Jaya Krishna KuruvaIn the present study, the acute toxicity of Chromium in fingerlings of Catla catla, an Indian major carp, was evaluated with renewal bioassay method. In vivo studies were designed to assess the extent of Micronucleus Assay, Comet Assay under the exposure of common heavy-metal compounds, namely, Chromium Nitrate, using Catla catla (2n = 20), as a test model. The laboratory … …American Journal of Environmental Sciences, Vol. 9, Issue 6 · 15 Jan 2014 · 7,200 accessesView PDFCite
Research ArticleOpen accessEvery word in the titleDNA Polymorphism of Bovine Pituitary-Specific Transcription Factor and Leptin Gene Between Iranian Bos indicus and Bos taurus CattleArash Javanmard, Nader Asadzadeh, Fatollah SarhadiProblem statement: Variations at DNA level contribute to the genetic characterization of livestock populations and this may help to identify possible hybridization events as well as past evolutionary trends. Approach: The leptin and Pit-1 are attractive candidate genes for production and reproduction traits in cattle. A total of 247 Animals from four breeds from two species of Iranian cattle populations … …American Journal of Agricultural and Biological Sciences, Vol. 5, Issue 3 · 30 Sep 2010 · 8,028 accessesView PDFCite
Research ArticleOpen accessEvery word in the titleThe Widely Used Diagnostics "DNA Microarray"-A ReviewR. Manoj KumarProblem statement: DNA microarray technique is one of the latest advances in the field of molecular biology and medicine. It is a multiplex technique used in combination of bioinformatics and statistical data analysis. Since, 1995, the technique offers the possibility of conducting tens or hundreds of thousands of simultaneous hybridizations. Approach: This increased experimental efficiency permits high throughput and whole … …American Journal of Infectious Diseases, Vol. 5, Issue 3 · 30 Sep 2009 · 7,789 accessesView PDFCite