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291 results for “RT-PCR”
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Research ArticleOpen accessIn the keywordsCASE REPORT OF A PATIENT WITH RARE P.ARG136CYS (APOE2*) MUTATION IN APOLIPOPROTEIN E GENEJana Lisyova, Jan Chandoga, Stanislav Oravec et al.Apolipoprotein E (apoE) is a polymorphic glycoprotein associated with plasma lipoproteins and plays an essential role in the metabolism and clearance of plasma lipids. ApoE can represent the major genetic risk factor in development of premature atherosclerosis, coronary heart disease and late-onset familial and sporadic Alzheimer’s Disease (AD). We present a case report of a 51-year old male with suspected … …OnLine Journal of Biological Sciences, Vol. 14, Issue 1 · 9 Jan 2014 · 7,295 accessesView PDFCite
Research ArticleOpen accessIn the keywordsRANDOMLY AMPLIFIED POLYMORPHIC DNA-A BRIEF REVIEWNandani Kumari, Saroj Kumar ThakurRAPD is a PCR based technique which involves the use of single arbitrary short primers (8-12 nucleotides), resulting in the amplification of many discrete DNA. The segments of DNA that are amplified are random. The technique was developed independently by two different laboratories and called as RAPD and AP-PCR (Arbitrary Primed PCR). This procedure detects nucleotide sequence polymorphisms in a … …American Journal of Animal and Veterinary Sciences, Vol. 9, Issue 1 · 2 Jan 2014 · 17,258 accessesView PDFCite
Research ArticleOpen accessIn the keywordsEnhancement of Nutritional Quality of Wheat (Triticum aestivum) by Metabolic Engineering of Isoflavone PathwayAhmed Mohamed El-Shehawi, Abdelmegid Ibrahim Fahmi, Mona Mohamed Elseehy, Hesham Ahmed NagatyIsoflavones are large group of secondary metabolites produced in legumes such as soybeans. They have essential biological functions as nutraceutical and health functions for human. They are involved in plant resistance to biotic and abiotic stresses, symbiotic relationship with nitrogen-fixing organisms and plant competition (allelopathy). In this report, isoflavonoids were expressed in wheat (Triticum aestivum) via introducing the key enzymes … …American Journal of Biochemistry and Biotechnology, Vol. 9, Issue 4 · 20 Dec 2013 · 7,135 accessesView PDFCite
Research ArticleOpen accessIn the keywordsPolymerase Chain Reaction Detection of Pasteurella multocida Type B: 2 in Mice Infected with Contaminated River WaterFaez Firdaus Jesse Abdullah, Mohammed Muqdad Khaleel, Lawan Adamu et al.Hemorrhagic septicemia is an acute, deadly disease of cattle and buffaloes associated with colossal economic loss in the livestock industry in the Asian regions particularly Malaysia. Therefore, this study was conducted to investigate on the Polymerase chain reaction detection of Pasteurella multocida type B: 2 in mice inoculated through different routes with river water contaminated with infected mice carcasses. Sixty … …American Journal of Animal and Veterinary Sciences, Vol. 8, Issue 3 · 17 Sep 2013 · 7,392 accessesView PDFCite
Research ArticleOpen accessIn the keywordsHerpetic Viral RetinitisHidetaka NomaHuman Herpes Virus (HHV) is a DNA virus and is the most important viral pathogen causing intraocular inflammation. HHV is classified into types 1-8. Among these types, HHV-1, HHV-2, HHV-3 Varicella Zoster Virus (VZV) and HHV-5 Cytomegalovirus (CMV) are known to cause herpetic viral retinitis, including acute retinal necrosis and CMV retinitis. Herpes viral retinitis can be diagnosed from characteristic … …Current Research in Virology, Vol. 2, Issue 1 · 29 Jul 2013 · 12,788 accessesView PDFCite
Research ArticleOpen accessIn the keywordsISOLATION AND CHARACTERIZATION OF HELICOBACTER PYLORI STRAINS FROM GASTRIC BIOPSIES OF ALGERIAN PATIENTSGuetarni Hassina, Bensoltane AhmedThe objective of this study is to isolate and characterize strains Helicobacetr pylori from gastric biopsies by upper gastrointestinal endoscopy. After a rapid urease test, two Helicobacter pylori strains isolated from four patients on blood agar. After extraction of DNA from these strains from homogenates of gastric biopsies, quantification by real-time PCR was performed to determine Helicobacter pylori on the … …OnLine Journal of Biological Sciences, Vol. 13, Issue 2 · 19 Apr 2013 · 7,264 accessesView PDFCite
Research ArticleOpen accessIn the keywordsHepatitis B Virus DNA Can be Amplified Directly From Dried Blood Spot on Filter PaperNaglaa Fathy Alhusseini, Mamdouh Z. Abadeer, Sheref M. El-TaherHepatitis B Virus (HBV) infections represent a major public health problem because of the ability of HBV to cause a chronic carrier state. Even though chronic carriers remain largely asymptomatic, a large number of these individuals subsequently develop cirrhosis and primary hepatocellular carcinomas. Dried Blood Spot (DBS) samples are a simple and inexpensive sampling method, especially useful for blood collection … …American Journal of Biochemistry and Biotechnology, Vol. 8, Issue 2 · 22 Jun 2012 · 8,454 accessesView PDFCite
Research ArticleOpen accessIn the keywordsRole of Progranulin Gene Expression as a Molecular Biomarker for Bladder CancerSalwa M. Abo El-khair, Mie A. Mohamed, Tamer E. AlkhiaryBladder cancer is the second most common cancer of the urinary system. Early diagnosis of this tumor and estimation of risk of future progression has a significant impact on prognosis. Although there are several molecular markers for the diagnosis and prognosis for this tumor, their accuracy is not ideal. Progranulin is a growth factor that may play a critical role … …American Journal of Biochemistry and Biotechnology, Vol. 8, Issue 2 · 22 Jun 2012 · 7,699 accessesView PDFCite
Research ArticleOpen accessIn the keywordsGanoderma Species Associated with Basal Stem Rot Disease of Oil PalmLing-Chie Wong, Choon-Fah J. Bong, A. S. IdrisProblem statement: Basal Stem Rot disease (BSR) is one of the most serious diseases that have been causing major losses in the oil palm industry in Southeast Asia, especially in Malaysia and Indonesia. Several species of Ganoderma have been reported pathogenic to oil palm, however, the diversity and differentiation of the Ganoderma species were not widely studied and the identity … …American Journal of Applied Sciences, Vol. 9, Issue 6 · 19 Mar 2012 · 9,631 accessesView PDFCite
Research ArticleOpen accessIn the keywordsDetection of Listeria monocytogenes in Ready-to-Eat Meat ProductsAsma M.M.A. Abdelgadir, Kunwar K. Srivastava, P. Gopal ReddyProblem statement: The objective of this study was to develop a rapid and sensitive method for the detection of Listeria monocytogenes and to screen large number of ready-to-eat (RTE) meat samples. Approach: A total of 250 fresh RTE meat samples of different varieties (chicken, turkey, beef, pork and cold cuts) were purchased from local grocery stores. Results: From the 250 … …American Journal of Animal and Veterinary Sciences, Vol. 4, Issue 4 · 30 Jan 2012 · 7,421 accessesView PDFCite