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59 articles for “Polymerase Chain Reaction (PCR)”
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Research ArticleOpen accessIn the abstractLemon Pepper Fruit Extract (Zanthoxylum acanthopodium DC.) Suppresses the Expression of Inflammatory Mediators in Lipopolysaccharide-Induced Macrophages In VitroYanti, Theodorus Eko Pramudito, Nerissa Nuriasari, Katarina JulianaProblem statement: Lemon pepper fruits (Zanthoxylum acanthopodium DC.; Rutaceae) have been used as a traditional source against stomach ache by Batak people in North Sumatera province, Indonesia. However, its scientific evidence for treatment of inflammatory disorders particularly gastritis has not been reported. Approach: Here, we investigated the inhibitory effects of Lemon Pepper Fruit Extract (LPFE) against inflammatory biomarkers by conducting … …American Journal of Biochemistry and Biotechnology, Vol. 7, Issue 4 · 14 Feb 2012 · 9,581 accessesView PDFCite
Research ArticleOpen accessIn the abstractStudy and Identification of Insulin-Like Growth Factor-I Gene Polymorphisms in Zel Sheep PopulationSeyed Mohsen Kazemi, Cyrus Amirinia, Hossein Emrani, Shahaboddin GharahveysiProblem statement: The IGFs play an important role in regulating somatic growth according to nutritional conditions. Polymorphisms of IGF gene are reported to be significantly associated with many traits. Approach: In these study 142 samples of DNA from Zel sheep were used for detecting the polymorphisms of promoter region of the Insulin-like Growth Factor-1 (IGF-1) gene. Therefore DNA extracted from … …American Journal of Animal and Veterinary Sciences, Vol. 6, Issue 4 · 13 Jan 2012 · 7,739 accessesView PDFCite
Research ArticleOpen accessIn the abstractGenetic Polymorphism Exon 9-11 at the Leptin Gene Receptor in Breeder Hens of Mazandaran Native FowlsHossein Ali Abbasi, Shahabodin Gharavysi, Rohullah AbdullahpourProblem statement: Leptin a 16 kD protein synthesized by white adipose tissue and involved in regulation of feed intake, energy balance, fertility and immune function. In order to evaluate the leptin gene receptor polymorphism, we have used a Restriction Fragment Length Polymorphism (RFLP) method. Approach: Blood samples were collected from randomly chosen 100 native fowls breeding station of Mazandaran. The … …American Journal of Animal and Veterinary Sciences, Vol. 6, Issue 2 · 17 Sep 2011 · 7,700 accessesView PDFCite
Research ArticleOpen accessIn the abstractDetection of Polymorphism at the Insulin Like Growth Factor-I Gene in Mazandaran Native Chicken using Polymerase Chain Reaction-Restriction Fragment Length Polymorphism MethodHossein Ali Abbasi, Mohsen KazemiProblem statement: Molecular genetics selection on individual genes is a promising method to genetically improve economically important traits in chickens. The Insulin like Growth Factor-I (IGF1) gene may play important roles in growth of multiple tissues, including muscle cells, cartilage and bone. Approach: In the present study polymorphism of the promoter and 5' untranslated region of IGF-1 gene of Mazandaran … …American Journal of Animal and Veterinary Sciences, Vol. 6, Issue 2 · 8 Aug 2011 · 7,627 accessesView PDFCite
Research ArticleOpen accessIn the abstractUsing Randomly Amplified Polymorphic DNA-Polymerase Chain Reaction of Barbodes spp. in ThailandKeeravit Petjul, Aphidech Sangdee, Bungorn Thaewnon-ngiwProblem statement: The genetic diversity of Barbodes species in Thailand is presently unavailable. The objectives of this study were to study external morphology and genetic diversity using Randomly Amplified Polymorphic DNA-Polymerase Chain Reaction (RAPD-PCR) of three Barbodes species; Barbodes altus, B. gonionotus and Barbodes sp. Approach: Two of twelve screened decanucleotide primers (OPA07 and UBC122), were selected for the genetic … …OnLine Journal of Biological Sciences, Vol. 11, Issue 1 · 6 Jul 2011 · 8,085 accessesView PDFCite
Research ArticleOpen accessIn the abstractDNA Polymorphism of Bovine Pituitary-Specific Transcription Factor and Leptin Gene Between Iranian Bos indicus and Bos taurus CattleArash Javanmard, Nader Asadzadeh, Fatollah SarhadiProblem statement: Variations at DNA level contribute to the genetic characterization of livestock populations and this may help to identify possible hybridization events as well as past evolutionary trends. Approach: The leptin and Pit-1 are attractive candidate genes for production and reproduction traits in cattle. A total of 247 Animals from four breeds from two species of Iranian cattle populations … …American Journal of Agricultural and Biological Sciences, Vol. 5, Issue 3 · 30 Sep 2010 · 8,024 accessesView PDFCite
Research ArticleOpen accessIn the abstractTransgenic Indian Cotton (Gossypium hirsutum) Harboring Rice Chitinase Gene (Chi II) Confers Resistance to Two Fungal PathogensM. Ganesan, P. Bhanumathi, K. Ganesh Kumari et al.Problem statement: The present investigation described a simple and reproducible protocol for transgenic cotton regeneration and characterization of chitinase (Chi II) gene expression against two different fungal pathogens in cotton. Approach: Transgenic cotton (Gossypium hirsutum cv. SVPR2) plants were produced by pCambia-bar-Chi II (13.8 kb) under the control of the CaMV 35S promoter, harbored in the strain LBA 4404 Agrobacterium … …American Journal of Biochemistry and Biotechnology, Vol. 5, Issue 2 · 30 Jun 2009 · 8,531 accessesView PDFCite
Research ArticleOpen accessIn the abstractGenetic Diversity of Red Chittagong Cattle Using Randomly Amplified Polymorphic DNA MarkersMohammad Masud Rana Mufti, Mst. Parvin Mostari, Gautam Kumar Deb et al.Problem statement: Red Chittagong Cattle (RCC) is a potential genetic resource in Bangladesh having dairy potentialities, high resistance to disease and survive harse environmental condition while require low input supply. This resource is now disappearing due to indiscriminate crossing with other local and exotic cattle. To conserve this resource, find out the genetic diversity of RCC is prime concern. Approach: … …American Journal of Animal and Veterinary Sciences, Vol. 4, Issue 1 · 17 Mar 2009 · 8,680 accessesView PDFCite
Research ArticleOpen accessIn the abstractDNA Code Word Design for DNA Computing with Real-Time Polymerase Chain ReactionMuhammad Faiz Mohamed Saaid, Zuwairie Ibrahim, Mohamad Shukri Zainal Abidin et al.Problem statement: A number of DNA computing models to solve mathematical graph problem such as the Hamiltonian Path Problem (HPP), Traveling Salesman Problem (TSP), and the Shortest Path Problem (SPP), have been proposed and demonstrated. Normally, the DNA sequences used for the computation should be critically designed in order to reduce error that could occur during a computation. We have … …Journal of Computer Science, Vol. 5, Issue 1 · 31 Jan 2009 · 7,502 accessesView PDFCite
Research ArticleOpen accessIn the abstractIdentification of Methicillin Resistant Staphylococcus aureus (MRSA) and Methicillin Resistant Coagulase-Negative Staphylococcus (CoNS) in Clinical SettingsM.A. Shorman, A.M. Atoom, N.M. Abuharfeil, A.M. Al-MajaliTo evaluate different methods for the identification of methicillin-resistant Staphylococci and their reliability, 112 Staphylococcal isolates (32 Staphylococcus aureus isolates and 80 coagulase negative Staphylococci isolates "CoNS") were collected from 118 nasal swab cultures and were subjected to three methods to detect oxacillin susceptibility of the isolates. The three methods were oxacillin disk diffusion method; the Epsilometer- test (E-test) and … …American Journal of Infectious Diseases, Vol. 4, Issue 2 · 30 Jun 2008 · 7,972 accessesView PDFCite