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79 results for “Polymerase Chain Reaction”
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Research ArticleOpen accessIn the abstractMomordica balsamina Fruit Extracts Enhances Selected Aspects of the Insulin Synthesis/Secretion PathwayAnanias Hodi Kgopa, Leshweni Jerry Shai, Motetelo Alfred MogalePrevious studies have suggested that crude plant extracts of the anti-diabetic medicinal plant, Momordica balsamina (MB) may exert their anti-diabetic effect through either enhancement of insulin sensitivity or inhibition of intestinal glucose absorption. However, the effects of these MB extracts on insulin synthesis and secretion are not well documented in the literature. Therefore, a main purpose of the current research … …American Journal of Biochemistry and Biotechnology, Vol. 16, Issue 4 · 17 Dec 2020 · 6,999 accessesView PDFCite
Research ArticleOpen accessIn the abstractDetection of tet Gene in Multidrug-Resistant Salmonella spp. Isolates from Layers and Broiler BreedersAgustin Indrawati, Leila Nur Azizah, I Wayan Teguh Wibawan et al.The present study was conducted with the following aims: (i) To detect the multidrug-resistant Salmonella spp. isolates recovered from faeces, litter and drinking water in layers and broiler breeders’ farms and (ii) to carried out the detection of the tet gene. A total of 21 Salmonella isolates were subjected to Polymerase Chain Reaction (PCR) assay to determine the presence of … …American Journal of Animal and Veterinary Sciences, Vol. 15, Issue 2 · 17 Jun 2020 · 7,007 accessesView PDFCite
Research ArticleOpen accessIn the abstractDetection of Commercial Fraud in Processed Meat Products Using Rapid TechniquesAli M. Ahmed, Takwa H. Ismail, Reham R. Abouelmaatti et al.Meat adulteration with different species has become a considerable problem worldwide. It means that meat products contain undeclared meat species; so, the meat ingredients are not consistent with the label. It has direct impact on public health, religious faith, fair-trades and food safety standers. This study focused on species adulteration detection in meat products by applying accurate, slandered and fast … …American Journal of Biochemistry and Biotechnology, Vol. 16, Issue 2 · 4 Jun 2020 · 7,817 accessesView PDFCite
Research ArticleOpen accessIn the abstractScreening of Blast Resistance Genes from South Tapanuli Rice Cultivars, North Sumatra, IndonesiaSaleha Hannum, Hesti Wahyuningsih, Riyanto Sinaga et al.Rice blast disease caused by a phytopathogenic fungus, Magnaporthe oryzae, has been considered as a major threat to global rice productivity, including Indonesia. South Tapanuli is a regency in North Sumatra, known for its intensive and significant rice production activities. A collection of 13 rice cultivars originating from the region, is claimed for its resistances against the rice blast disease … …OnLine Journal of Biological Sciences, Vol. 20, Issue 2 · 25 May 2020 · 7,537 accessesView PDFCite
Research ArticleOpen accessIn the abstractThe Role of SLC22A4 Gene Polymorphisms in the Response to Salbutamol in Asthmatic PatientsAlshymaa A. Ahmed, Nora M. Said, Alia A. El Shahaway, Nagwan A. IsmailResponse to Bronchodilators is influenced by many factors including genetics. To evaluate the influence of SLC22A4 gene polymorphisms (rs3792876 and rs2073838) on the response to inhaled salbutamol in asthmatic patients, bronchodilator response was assessed in 180 bronchial asthma patients via measuring changes of the Forced Expiratory Volume in one second (FEV1) after the administration of inhaled salbutamol and genotyping for … …American Journal of Immunology, Vol. 15, Issue 1 · 30 Jun 2019 · 7,470 accessesView PDFCite
Research ArticleOpen accessIn the abstractGenetic Diversity in Merozoite Surface Protein.1 of Plasmodium falciparum in Highlands: Wamena Papua IndonesiaRosye Hefmi Rechnelty Tanjung, Yulius Sarungu, Meidy Johana Imbiri et al.Malaria is the first killer as well as endemic disease in highland, lowland and coastal areas of Papua. In 2016 there were 160 thousands cases noted and 80% of the cases were caused by Plasmodium falciparum. Total sample for this study were provided by a clinical trial conducted from May to June 2018 in the regional hospital of Puncak Jaya, … …American Journal of Infectious Diseases, Vol. 14, Issue 4 · 11 Jan 2019 · 7,753 accessesView PDFCite
Research ArticleOpen accessIn the abstractVariations in Pathogenicity and Molecular Characterization of Infectious Bursal Disease Virus (IBDV) In EgyptMahmoud E. Sedeik, Ashraf M. Awad, Heba Rashed, Wael K. ElfeilInfectious bursal disease consider a vital threat to poultry industry worldwide and It has special potentiality in Egypt as the country become endemic with several clades from avian influenza from both types highly pathogenic (H5N1 and H5N8) and low pathogenic one (H9N2). The Egyptian government depends on the inactivated vaccine strategy to control avian influenza and any infection with IBD … …American Journal of Animal and Veterinary Sciences, Vol. 13, Issue 2 · 18 May 2018 · 8,479 accessesView PDFCite
Research ArticleOpen accessIn the abstractCariogenic Pathogen Scardovia Wiggsiae Screening Among Pediatric Orthodontic Patients: A Pilot StudyWeston Milne, Ghazaleh Rezaei, Adam Whiteley, Karl KingsleyDental caries remains one of the most prevalent oral health diseases in the United States, affecting nearly half of all children and a majority of adults. Most medically important cariogenic bacteria, including Streptococcus, Lactobacillus, Actinomyces and Veillonella species are well known, although recent evidence has identified the new cariogenic pathogen Scardovia wiggsiae (S. wiggsiae) among children and minorities with severe … …Current Research in Dentistry, Vol. 9, Issue 1 · 6 Oct 2017 · 11,869 accessesView PDFCite
Research ArticleOpen accessIn the abstractSex Identification and the Sex-Related Genes of Nipponia NipponFeilong Sun, Xuanfu Huang, Shuiyun YangSex identification in birds is necessary for studying their ecology, behavior and population dynamics and structure. This study was carried out to determine the sex of Nipponia nippon by a Polymerase Chain Reaction (PCR) method. The sexing universal primers of non-ratite birds were employed to amplify the sexing related genes and identify the sex of each individual. A 657 bp … …American Journal of Biochemistry and Biotechnology, Vol. 13, Issue 2 · 30 May 2017 · 7,841 accessesView PDFCite
Research ArticleOpen accessIn the abstractImpact of Acute Bronchiolitis on Cardiac Functions and Serum microRNA-122 and 499Mona Abd El-Gelel Mohamed, Khalid Mahmoud ZayedBronchiolitis is an acute inflammatory injury of the bronchioles that is usually caused by a viral infection, most commonly Respiratory Syncytial Virus (RSV). This study aimed to verify the direct injurious effect of acute bronchiolitis on the myocardium and serum level of microRNA-122 and 499 (miR-122 and miR-499) of infected infants. Serum lactate dehydrogenase (LDH), creatine phosphokinase-isoenzyme MB (CK-MB) and … …American Journal of Infectious Diseases, Vol. 12, Issue 1 · 26 Jan 2016 · 7,250 accessesView PDFCite