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92 results for “Incubation”
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Research ArticleOpen accessIn the abstractHeterologous Expression and Biochemical Characterization of Lipase from Burkholderia cepacia Lu10-1Yao Zhang, Lu Wang, Yuanda SongEven though lipase genes from a variety of microorganism have been cloned and over-expressed, the prospective lipase resources for commercial production and industry application are still limited. In the present study, a lipase from Burkholderia cepacia Lu10-1 is heterologously over-expressed in Escherichia coli strain BL21(DE3) and purified to homogeneity. The molecular weight of the recombinant lipase from B. cepacia Lu10-1 … …American Journal of Biochemistry and Biotechnology, Vol. 13, Issue 4 · 23 Dec 2017 · 8,766 accessesView PDFCite
Research ArticleOpen accessIn the abstractThe Effect of Lactobacillus Plantarum and Bacterial Peptidoglycan on the Growth of Mouse Tumors in vivo and in vitroArpa Aintablian, Dalal F. Jaber, Mary Ann Jallad, Alexander M. AbdelnoorSome members of the microbiota have been shown to be effective strategies for inhibiting tumor growth through stimulation of host anti-tumor immune responses. Anti-tumor immune effects were observed when Lactobacillus plantarum (Lp), a member of the gut microbiota, was used to treat colorectal cancer in mice. Moreover, constituents of bacteria, including peptidoglycan (PG), have been shown to exhibit tumoricidal effects. … …American Journal of Immunology, Vol. 13, Issue 3 · 23 Sep 2017 · 8,451 accessesView PDFCite
Research ArticleOpen accessIn the abstractQuercetin Promotes the Expression of Genes Involved in Phagocytosis in Bovine NeutrophilsSuphakit Srikok, Sukij Nambut, Kanruethai Wongsawan, Phongsakorn ChuammitriThis study was conducted to explore the effect of Quercetin (QH) on phagocytosis of bovine neutrophils. Neutrophils were isolated from fifteen multiparous Holstein cows. We in vitro treated neutrophils with PBS or 50 µM of Quercetin (QH) or Cytochalasin B (CytB), as phagocytosis inhibitor, prior to monitoring phagocytosis of Escherichia coli by flow cytometry and microscopic examination. Additionally, the expressions … …American Journal of Animal and Veterinary Sciences, Vol. 12, Issue 2 · 2 Aug 2017 · 8,124 accessesView PDFCite
Review ArticleOpen accessIn the abstract"Ebola Ça Suffit!" is not enough to Prove Efficacy of an Ebola VaccineDavid R CroweA recently published Ebola vaccine trial claimed an almost perfect result with no cases of Ebola after the vaccine had time to take effect - a vaccine efficacy of 100%. This article is a detailed review of the information published about the trial and, particularly, this claim. The trial design was sub-optimal because randomization was only partial (and one treatment … …American Journal of Immunology, Vol. 13, Issue 3 · 8 Jul 2017 · 17,832 accessesView PDFCite
Research ArticleOpen accessIn the abstractEnzymatic Hydrolysis of Pretreated Kenaf using a Recombinant Xylanase: Effects of Reaction Conditions for Optimum Hemicellulose HydrolysisNur Izyan Wan Azelee, Jamaliah Md Jahim, Ahmad Fauzi Ismail et al.An efficient hemicellulose hydrolysis has now become a crucial step for xylooligosaccharides production for food additives and nutraceuticals industries. In this study, xylanase hydrolysis of pretreated hemicellulosic kenaf (Hibiscus cannabinus) for substantial hemicellulose hydrolysis was demonstrated. Experiments were performed primarily to investigate the effect of several critical reaction conditions towards the enzymatic hydrolysis of the pre-treated kenaf. The effects of … …American Journal of Agricultural and Biological Sciences, Vol. 11, Issue 2 · 28 Apr 2016 · 7,819 accessesView PDFCite
Research ArticleOpen accessIn the abstractStage Specificity of Eurycomanone Isolated from Eurycoma longifolia on Plasmodium falciparum CyclesEti Nurwening Sholikhah, Mahardika Agus Wijayanti, Ratna Asmah Susidarti et al.Eurycomanone is the most active compounds in the roots of Eurycoma longifolia and shown to have in vitro antiplasmodial activity. However, the stage of Plasmodium falciparum cycles which are sensitive to eurycomanone have not been investigated. This study was conducted to investigate stage specificity of eurycomanone at various stages of P. falciparum life cycles. Stage specificity of eurycomanone at various … …American Journal of Pharmacology and Toxicology, Vol. 11, Issue 1 · 16 Jan 2016 · 7,745 accessesView PDFCite
Research NotesOpen accessIn the abstractPolymerisation and Oxidation of Docosahexaenoic AcidBrian Mundell Ross, Shelly Lynn Browning, Adrian McKeeOils rich in polyunsaturated fatty acids, such as linseed oil, can oxidise to form polymers. These can be used as protective coatings and act as an alternative to those derived from petrochemicals. Such renewable source oils usually require the addition of metal ions as catalysts of the polymerisation process and/or the partial pre-oxidation of the oil before use. We hypothesised … …American Journal of Biochemistry and Biotechnology, Vol. 12, Issue 1 · 11 Dec 2015 · 7,621 accessesView PDFCite
Research ArticleOpen accessIn the abstractInfluence of Cadmium and Glucose on Soil Microbial CommunitiesAliya Gilmullina, Polina Galitskaya, Svetlana SelivanovskayaA laboratory-based simulation of exposure of the soil community in a gray forest soil to cadmium and glucose has been conducted. It was established that while growing in a fertile medium microbial communities isolated from a soil sample contaminated with cadmium have a substantially increased phase of growth delay and there is a reduction in total growth. While analyzing communities … …American Journal of Applied Sciences, Vol. 12, Issue 10 · 6 Nov 2015 · 6,652 accessesView PDFCite
Review ArticleOpen accessIn the abstractVaccine against Jembrana Disease Virus Infection: A Summary FindingsAsmarani Kusumawati, Tenri Ashari Wanahari, Pudji Astuti et al.Jembrana disease constitutes the main concern in cattle industry especially in Indonesia and Australia as it has caused important economic losses due to mortality of cattle. The pathology of the disease is unusual for a lentivirus infection as it is associated with a severe, often lethal disease syndrome and a short incubation period in cattle. For lack of efficient medical … …American Journal of Immunology, Vol. 11, Issue 3 · 2 Sep 2015 · 7,854 accessesView PDFCite
Research ArticleOpen accessIn the abstractOptimization of Cultural Conditions for Production of Exopolysaccaride by Halomonas marina HMA 103 under Batch-CultureArundhati Pal, Amrita Biswas, Sriya Chatterjee, A. K. PaulA moderately halophilic bacterium, Halomonas marina HMA 103 (MTCC 8968) accumulating intracellular polyesters also produced significant amount of exopolysaccharides (EPS) under batch-culture. Production of EPS by the bacterium was more or less parallel with the growth in modified basal synthetic medium. During growth, it produced 0.46 g/L of EPS after 50 h of incubation and was accompanied by the complete … …Current Research in Microbiology, Vol. 6, Issue 2 · 27 Jul 2015 · 7,936 accessesView PDFCite