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89 results for “Culture Medium”

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Research ArticleOpen accessIn the abstractEffect of Fetal Serum and Follicular Liquor Supplementation on the In Vitro Production of Bovine EmbryosClara Larocca, Yael Filipiak, William Perez, Juan CalvoTo optimize In Vitro Fertilization (IVF) results in bovines by lowering costs, comparing the efficiency of Fetal Calf Serum (FCS) (high cost) with respect to bovine Follicular Fluid (bFF) (low cost) and by quantifying embryo production. Cumulus-Oocyte-Complexes (COC) obtained from a slaughter house, transported at 37°C in saline solution, were classified according to their quality in A, B, C and … …American Journal of Animal and Veterinary Sciences, Vol. 7, Issue 3 · 9 Aug 2012 · 7,842 accessesView PDFCite
Research ArticleOpen accessIn the abstractBACTERIAL ISOLATES OF MARINE COAST AS COMMERCIAL PRODUCER OF PROTEASES. Das, I. Mukherjee, M. Sudarshan et al.The objective of this work was to explore and exploit the extracellular protease secreting marine microbial biodiversity of the eastern coastal region of India. Culture dependent method was applied for isolation of microbes from the marine coast of West Bengal (Digha and Mandarmani) and Andhra Pradesh (Vizag) in India. Six protease secreting isolates were screened using casein hydrolysing property as … …OnLine Journal of Biological Sciences, Vol. 12, Issue 3 · 8 Aug 2012 · 8,799 accessesView PDFCite
Research ArticleOpen accessIn the abstractInulinase Production by Penicillium citrinum ESS in Submerged and Solid-State CulturesCynthia Lafuente-Castaneda, Gabriela Martinez, Juan C. Contreras-Esquivel et al.Problem statement: This work reports the Penicillium citrinum ESS strain as a new source of inulinase (2,1-β-D fructanhydrolase, E.C. 3.2.1.7). Approach: The enzyme was produced in both, Solid-State (SSC) and Submerged Culture (SmC) using a basal medium added either with inulin or sucrose (5 gL-1). Results: The culture grew faster in SmC than in SSC with both carbon sources; however, … …American Journal of Agricultural and Biological Sciences, Vol. 7, Issue 2 · 13 Mar 2012 · 8,394 accessesView PDFCite
Research ArticleOpen accessIn the abstractEnhanced Production of Extracellular Alkaline Lipase by an Improved Strain of Pseudomonas aeruginosa MTCC 10,055Deepali Bisht, Santosh Kumar Yadav, Nandan Singh DarmwalProblem statement: Lipases are industrially important enzymes having applications in numerous industries. For easy commercialization it is necessary to produce lipases at industrial level which could be achieved by strain improvement and medium formulation. Approach: In the present study strain improvement of Pseudomonas aeruginosa MTCC 10,055 was done by chemical mutagenesis using mutagen 4-nitroquinoline1-oxide for alkaline lipase production. Different fermentation … …American Journal of Applied Sciences, Vol. 9, Issue 2 · 24 Nov 2011 · 8,767 accessesView PDFCite
Research ArticleOpen accessIn the abstractEffect of Nitrogen on Growth and Lipid Content of Chlorella pyrenoidosaSubhasha Nigam, Monika Prakash Rai, Rupali SharmaProblem Statement: In today’s scenario, microalgae are widely recognized as a promising source for biofuel production as a renewable source of energy. They are exceedingly rich in oil, which can be converted to biofuel. This paper deals with one of the method to enhance the lipid content in microalgae. Approach: Microalga, Chlorella pyrenoidosa, was grown autotrophically in batch culture and … …American Journal of Biochemistry and Biotechnology, Vol. 7, Issue 3 · 22 Sep 2011 · 12,293 accessesView PDFCite
Research ArticleOpen accessIn the abstractEffects of Teaching Literature on Culture Learning in the Language ClassroomChittra Muthusamy, Rasaya Marimuthu, Elangkeeran SabapathyProblem statement: The role of literature in enhancing readers’ cultural understanding in the language classroom was explored. It was a part of an extensive research which focused mainly on language learning and creativity. It is argued that the interface of language, literature and culture are at the forefront of present-day language and literature learning and this facilitates inter-racial, intra-racial and … …American Journal of Applied Sciences, Vol. 8, Issue 8 · 19 Jul 2011 · 7,779 accessesView PDFCite
Research ArticleOpen accessIn the abstractKnowledge Management Acceptance: Success Factors amongst Small and Medium-Size EnterprisesChristine Nya Ling TANProblem statement: The value and importance of knowledge, as seen by numerous organisations today, does without a doubt play a crucial role in the current ever-challenging and aggressive business environment. As a result, businesses that aspire to be labelled as being successful and competitive need to seek and find better ways to improve their firms’ performance. Hence, Knowledge Management (KM), … …American Journal of Economics and Business Administration, Vol. 3, Issue 1 · 7 Jan 2011 · 8,378 accessesView PDFCite
Research ArticleOpen accessIn the abstractEffect of Plant Growth Regulators on Callus, Cell Suspension and Cell Line Selection for Flavonoid Production from Pegaga (centella asiatica L. urban)Suat Hian Tan, Radzali Musa, Arbakariya Ariff, Mahmood MaziahProblem statement: Considering pegaga medicinal properties and over-exploitation, the requirement for a tissue culture technique as an alternative production system was crucial. Approach: Investigation of cell suspension culture response to different plant growth regulators (PRGs) for flavonoid production from elite cell line was carried out. Callus cultures were initiated from the leaf explants of Centella asiatica on Murashige and Skoog … …American Journal of Biochemistry and Biotechnology, Vol. 6, Issue 4 · 31 Dec 2010 · 11,261 accessesView PDFCite
Research ArticleOpen accessIn the abstractSynergistic Effect of Insulin on in vitro Development of Immature Bovine OocytesMojtaba Dashtizad, Abd Wahid Haron, Rosnina Yusoff et al.Problem statement: Development of efficient culture system to support embryonic development would be valuable when quality of produced embryos was important. However, the rate of bovine embryo production in vitro was still lower than expected. Present study, including of three experiments, was carried out to investigate the effect of insulin on nuclear maturation and subsequent development of immature bovine oocytes … …American Journal of Animal and Veterinary Sciences, Vol. 5, Issue 4 · 31 Dec 2010 · 10,438 accessesView PDFCite
Research ArticleOpen accessIn the abstractFungal Culture Systems for Production of Antioxidant Phenolics Using Pecan Nut Shells as Sole Carbon SourceMiguel A. Medina, Ruth E. Belmares, Antonio Aguilera-Carbo et al.Problem statement: Many agro-industrial wastes have little or none utilization, when these materials could be a very rich source of several value-added compounds, such as: the pecan nut shells, which contain Antioxidant Phenolic (AP) molecules like tannins. Approach: In this study, a bioprocess for the liberation of AP from Pecan Nut Shells (PNS) was described. A chemical characterization of raw … …American Journal of Agricultural and Biological Sciences, Vol. 5, Issue 3 · 30 Sep 2010 · 8,045 accessesView PDFCite