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270 results for “Biochemical”
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Research ArticleOpen accessEvery word in the titleHeterologous Expression and Biochemical Characterization of Lipase from Burkholderia cepacia Lu10-1Yao Zhang, Lu Wang, Yuanda SongEven though lipase genes from a variety of microorganism have been cloned and over-expressed, the prospective lipase resources for commercial production and industry application are still limited. In the present study, a lipase from Burkholderia cepacia Lu10-1 is heterologously over-expressed in Escherichia coli strain BL21(DE3) and purified to homogeneity. The molecular weight of the recombinant lipase from B. cepacia Lu10-1 … …American Journal of Biochemistry and Biotechnology, Vol. 13, Issue 4 · 23 Dec 2017 · 8,766 accessesView PDFCite
InvestigationOpen accessEvery word in the titleMorphogenetic and Biochemical Study on University Common Bean CollectionBeibitgul Zhumabaeva, Erika Dzhangalina, Zaure Aytasheva et al.This research has been conducted to reveal the outcomes of rotation of crops in mountain and steppe districts near Almaty. The main morphogenetic characteristics have been investigated for the collection of representatives of common bean, Phaseolus vulgaris L. by using samples from various countries such as Kazakhstan, USA, China, Czech Republic, Poland, Turkey and Russia under different climate and soil … …OnLine Journal of Biological Sciences, Vol. 17, Issue 4 · 28 Nov 2017 · 7,051 accessesView PDFCite
Research ArticleOpen accessEvery word in the titleIntracellular L-Asparaginase from Bacillus sp. PG02: Purification, Biochemical Characterization and Evaluation of Optimum pH and TemperatureFatemeh Izadpanah Qeshmi, Mahsa Rahimzadeh, Sedigheh Javadpour, Manijeh PoodatBacterial L-asparaginases are amidohydrolases that act on L-asparagine and produce L-aspartate and ammonia. These enzymes have been used in treatment of lymphoblastic leukemia. In the present study, a novel strain, Bacillus sp. PG02 was explored for the production of intra-cellular L-asparaginase enzyme. The nitrogen source for L-asparaginase production was L-asparagine. New intracellular L-asparaginase was purified using ion exchange chromatography and … …American Journal of Biochemistry and Biotechnology, Vol. 12, Issue 1 · 26 Nov 2015 · 7,484 accessesView PDFCite
Research ArticleOpen accessEvery word in the titlePhysiological and Biochemical Evaluation of Fe-Efficiency in Fe-Deficient Maize GenotypesEntesar M. Essa, Sahar M. Zaghloul, Z.A. Salama et al.Iron (Fe) deficiency is prevalent particularly in calcareous soils of arid and semiarid regions. The present study aimed to investigate the response of eight local maize genotypes to Fe deficiency. In addition, a Fe- efficient (WF9) and a Fe-inefficient (ys1) indicator genotypes were used in this study. All genotypes were grown in growth chamber for 21 days in two levels … …American Journal of Agricultural and Biological Sciences, Vol. 10, Issue 1 · 22 Apr 2015 · 7,283 accessesView PDFCite
Research ArticleOpen accessEvery word in the titleBiochemical Efficacy of Nigella Sativa Oil and Metformin on Induced Diabetic Male RatsYassmina K. Mahmoud, Sherif Y. Saleh, Abd El Rehim A. El Ghannam, Ibrahim A. IbrahimThis study was carried out to investigate possible beneficial effects of Nigella sativa oil supplementation to diabetic rats. Fifty male Sprague-Dawley rats were randomly divided into five groups (n = 10/group) as follows: group I (normal control), group II (diabetic control), group III (diabetic rats treated with metformin), group IV (diabetic rats treated with metformin and Nigella sativa oil) and … …American Journal of Animal and Veterinary Sciences, Vol. 9, Issue 4 · 24 Dec 2014 · 7,902 accessesView PDFCite
Research ArticleOpen accessEvery word in the titleBiochemical Alterations Induced by Amylin in Wistar RatsMohamed Mohamed Soliman, Zein Shaban IbrahimAmylin is a peptide hormone synthesized in β cells of pancreas together with insulin in response to glucose and nutrients. Amylin controls food intake and body weight but the exact mechanism is still unknown. This study aimed to examine the effects of amylin injection on hormones, metabolites and cytokines expression in rats. Wistar rats were injected amylin Intraperitoneally (IP) (10 … …American Journal of Pharmacology and Toxicology, Vol. 8, Issue 2 · 7 Jun 2013 · 6,771 accessesView PDFCite
Research ArticleOpen accessEvery word in the titleFluctuation of Brain Tissue Oxygen Partial Pressure: A Biochemical Landmark in the Arctic Ground Squirrel's Spontaneous ArousalYi Long Ma, Shu Fen Wu, Lawrence K. DuffyHibernation in the Arctic ground Squirrel (AGS) is a regulated, adaptive response to arctic environmental conditions. Problem statement: Regional brain Blood Flow (rCBF) has been observed to increase dramatically during arousal in hibernators. However, the real time dynamic change in PtO2 during arousal has not been studied, we hypothesized that PtO2 is Interdependent of Tbrain and a key component in … …American Journal of Biochemistry and Biotechnology, Vol. 7, Issue 4 · 1 Feb 2012 · 7,605 accessesView PDFCite
Research ArticleOpen accessEvery word in the titleBiochemical Studies of Lipase from Germinating Oil Seeds (Glycine max)P. P. Gadge, S. D. Madhikar, J. N. Yewle et al.Problem statement: Lipase is one of the important enzymes in food, pharmaceutical, detergent and biofuels industries. Search for the lipase with distinct features, possibly from germinating seeds, is of interest for industrial applications. Approach: The lipase produced by soybean oil seeds was partially purified and characterized in terms of the optimal pH and temperature for activity as well as substrate … …American Journal of Biochemistry and Biotechnology, Vol. 7, Issue 3 · 19 Jan 2012 · 9,706 accessesView PDFCite
Research ArticleOpen accessEvery word in the titleBiochemical Studies on Recombinant Human Isobutyryl-CoA DehydrogenaseNasser E. Ibrahim, Al-Walid A. MohsenProblem statement: Human Isobutyryl-CoA Dehydrogenase (IBD) is member of the Acyl-CoA Dehydrogenases family. It is involved in Val metabolism. In this study we modified the purification method of the IBD and further spectroscopic characterization was conducted. Approach: Using DTT in the buffers during the purification, IBD purified to homogeneity by different chromatographic steps. Reactivity of Cys residues were measured using … …American Journal of Biochemistry and Biotechnology, Vol. 7, Issue 2 · 12 Aug 2011 · 7,541 accessesView PDFCite
Research ArticleOpen accessEvery word in the titleEffect of Maturity Stage of Papaya Maradol on Physiological and Biochemical ParametersLaura E. Gayosso-García Sancho, Elhadi M. Yahia, Miguel Angel Martínez-Téllez, Gustavo Adolfo González-AguilarProblem statement: Nowadays, the worldwide increase in diseases has motivated consumers to increase the intake of fruits and vegetables, in response to various research reports indicating that fruits and vegetables can help prevent certain types of illnesses, due to their potentially high antioxidant properties. We evaluated the effect of the stage of ripeness of papaya fruit (Carica papaya L.) on … …American Journal of Agricultural and Biological Sciences, Vol. 5, Issue 2 · 30 Jun 2010 · 11,270 accessesView PDFCite