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63 results for “Allele”

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Research ArticleOpen accessIn the abstractCreation of Tetracycline Regulatable Knockouts of ORFF Gene from the LD1 Locus of Leishmania donovaniBindu Sukumaran, Manju Jain, Peter J. Myler et al.The tetracycline repressor/operator system was adapted for use in Leishmania donovani. In order to investigate the possible importance and function of open reading frame F (ORFF) in Leishmania donovani strain AG83 that has a single copy of ORFF in the LD1 locus on chromosome 35, we have attempted to generate tetracycline regulatable single allele mutants of ORFF. Leishmania cells expressing … …American Journal of Biochemistry and Biotechnology, Vol. 3, Issue 4 · 31 Dec 2007 · 6,829 accessesView PDFCite
Research ArticleOpen accessIn the abstractQTL Mapping Using Multiple Markers SimultaneouslyD. Kolbehdari, J.A.B. RobinsonMethods for detecting and locating a single QTL within a 10 cM region of DNA using 10 equally spaced SNP markers were compared. The QTL was assumed to be bi-allelic and located between markers 5 and 6. Monte Carlo simulation of a granddaughter design with 30 sires and 400 sons was used. Linear regression nested within sire using either two … …American Journal of Agricultural and Biological Sciences, Vol. 2, Issue 3 · 30 Sep 2007 · 7,110 accessesView PDFCite
Research ArticleOpen accessIn the abstractGenetic Diversity of Common Wild Wheat Species in Armenia Using ISSR MarkersManvel Badalyan, Tatevik Aloyan, Varya Dilanyan et al.Armenia is one of the primary genealogical centers of numerous crops, the wild relatives of many of which are still found in Armenia today. Population limits of three out of 4 species of wild relatives of endemic wheat in Armenia (T. araraticum Jakubz, T. urartu Tumanian ex Gandilyan, T. boeoticum Boiss.) have been reduced in recent decades as a result … …OnLine Journal of Biological Sciences, Vol. 23, Issue 3 · 15 Aug 2023 · 5,217 accessesView PDFCite
Research ArticleOpen accessIn the abstractMarking of Genetic Resistance to Chlamydia, Brucellosis and Mastitis in Holstein Cows by Using Polymorphic Variants of LTF, MBL1 and TLR9 GenesIndira Beishova, Birzhan Nurgaliyev, Alena Belaya et al.The purpose of the research is to study the association of polymorphic variants of the Toll-Like Receptor 9 (TLR9), Mannose-Binding Lectin 1 (MBL1), and Lactoferrin (LTF) genes with the incidence of chlamydia, brucellosis and mastitis in Holstein cows. Samples of Holstein cows with established diagnoses of chlamydia, brucellosis, and mastitis were characterized by the nature of the correspondence of the … …American Journal of Animal and Veterinary Sciences, Vol. 18, Issue 2 · 6 May 2023 · 6,157 accessesView PDFCite
Research ArticleOpen accessIn the abstractAssessment of Genetic Diversity among Elite Breeding Lines of Arabica Coffee (Coffea arabica L.) in Ethiopia using Simple Sequence Repeats MarkersTadesse Benti, Endale Gebre, Kassahun Tesfaye et al.Despite being the center of origin and genetic diversity, there has beenlimited application of molecular markers in arabica coffee breeding inEthiopia. In this study, the extent of genetic diversity and relationships of48 elite breeding lines of arabica coffee from different geographical originswere evaluated using 14 SSR markers. The SSR markers amplified 105 alleles ofwhich 104 are polymorphic with an average … …American Journal of Biochemistry and Biotechnology, Vol. 18, Issue 4 · 25 Nov 2022 · 6,009 accessesView PDFCite
Research ArticleOpen accessIn the abstractGenetic State of Pulsatilla Patens (L.) Mill. Coenopopulations Based on DNA LabelingRustem Saltanovich Beishov, Gulnar Sultangazina, Indira Saltanovna Beishova et al.In the article, the results of studying the gene pools of the Kazakhstan coenopopulations of a rare plant species Pulsatilla patens (L.) Mill. are shown. The biomaterial taken from five coenopopulations located in Northern Kazakhstan has been used as samples. To identify the multilocus DNA polymorphism, the authors have used ISSR (Inter Simple Sequence Repeats, or intermicrosatellite analysis) labeling. The … …OnLine Journal of Biological Sciences, Vol. 21, Issue 1 · 2 Apr 2021 · 5,944 accessesView PDFCite
Research ArticleOpen accessIn the abstractValidation of SSR markers associated with Submergence Tolerance in Rice (Oryza sativa L.)Kirill V. Azarin, Alexander V. Usatov, Andrey V. Alabushev et al.The present work is devoted to the validation of SSR markers associated with submergence tolerance (Sub1A) QTLs in rice. It was shown that only microsatellite markers RM 7481 differentiates donor lines and recipient variety. Based upon the results of DNA analysis with the marker of the RM 7481 of the F2-plants, the 14 homozygotes by the locus Sub1A, 40 samples … …American Journal of Agricultural and Biological Sciences, Vol. 11, Issue 4 · 19 Dec 2016 · 7,634 accessesView PDFCite
Research ArticleOpen accessIn the abstractDevelopment, Polymorphism and Cross-Species Transferability of Genomic SSR Markers in Duabanga Moluccana, an Indigenous Tree Species from SarawakKit-Siong Liew, Wei-Seng Ho, Shek-Ling Pang, Julaihi AbdullahIn this study, we used ISSR-suppression methods to develop a set of SSR markers for Duabanga moluccana. It is an indigenous fast growing tropical tree species. A total of 44 SSR regions were identified and specific primer pairs were designed. The SSR motifs contained perfect compound with 24 (54.5%) occurrences, followed by the imperfect compounds with 8 (18.2%), simple perfect … …OnLine Journal of Biological Sciences, Vol. 16, Issue 1 · 26 Jan 2016 · 7,784 accessesView PDFCite
Research ArticleOpen accessIn the abstractSSR Analysis of Maternal and Paternal Lines Selected in the Don Region (Russia)N. V. Markin, A. V. Usatov, V. N. Vasilenko et al.Evaluation of DNA polymorphisms of breeding material of sunflower from L.A. Zhdanov Don Experimental Station of oil Crops of V.S. Pustovoit All-Russian Research Institute of Oil Crops represented by 17 maternal (CMS) lines and 12 paternal (Rf) lines was conducted. There were identified 35 allelic variants of CMS lines and 42 allelic variants of Rf lines with use of 11 … …American Journal of Agricultural and Biological Sciences, Vol. 11, Issue 1 · 16 Jan 2016 · 7,555 accessesView PDFCite
Research ArticleOpen accessIn the abstractINTERVENTION OF PHARMACOGENETIC PROFILING IN THERAPEUTICS OF KASHMIRI POPULATION, A LESSON LEARNED FROM CYP3A5*3 VARIANTAshaqullah Bhat, Aadil Wani, Rajinder Kaur et al.Human Cytochrome P450 3A, a variant of Cytochrome P-450- dependent monooxygenases is a major drug metabolizing enzyme. CYP3A has evolved to catalyze the biotransformation of its substrate. A great deal of inter-individual variation in expression levels exists for CYP3A5 gene. The common polymorphism in CYP3A5 that induces a cryptic splice site has been shown to cause a significant reduction in … …OnLine Journal of Biological Sciences, Vol. 14, Issue 3 · 10 Jul 2014 · 7,215 accessesView PDFCite