53 results for “enzymatic activity”

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Research ArticleOpen accessIn the abstractEFFECT OF PIOGLITAZONE ON HEPATIC ULTRA-STRUCTURE AND METABOLIC CHANGES INDUCED BY A HIGH SUCROSE DIET IN RATSMohamed D. Morsy, Ahmed S. Khashaba, Ahmed I. Agamy, Refaat A. EidPioglitazone (Pio) is a PPAR-γ agonist insulin sensitizer has anti-inflammatory activity. Our novel aspect was to investigate its hepatic anti-inflammatory activity at the level of ultra-structure and enzymatic changes in a high sucrose diet animal model. Forty male Sprague Dawley rats were divided into four equal groups: Control; control Pio; high sucrose diet; high sucrose diet Pio treated groups. Fourteen … …American Journal of Applied Sciences, Vol. 11, Issue 7 · 23 Apr 2014 · 6,732 accessesView PDF Cite

Research ArticleOpen accessIn the abstractCELL DETACHMENT BY PROLYL-SPECIFIC ENDOPEPTIDASE FROM WOLFIPORIA COCOSNicole Mika, Katharina Cierpka, Michaela C. Lange et al.As requirements for Advanced Therapy Medicinal Product (ATMP) production differ from other production processes (e.g., therapeutic protein production), cell detachment is often a crucial step for the process success. In most cases, cell detachment is done enzymatically. Although many peptidases are established in cell culture in R&D, e.g., Trypsin as gold standard, many of them seem to be unsuitable in … …American Journal of Biochemistry and Biotechnology, Vol. 10, Issue 1 · 8 Feb 2014 · 8,157 accessesView PDF Cite

Research ArticleOpen accessIn the abstractAroclor 1254 Toxicity to Mice Liver Membrane AtpasesShweta Pathak, Rahul KunduPresent communication reports the sub-acute dose and exposure duration dependent effects of Aroclor 1254 on total, Na+, K+, Ca++ and Mg++-ATPases of the mouse liver cells. The study tests two hypotheses, (a) whether the sublethal dose or the exposure duration dependent in vivo exposure of hydrophobic PCB (Aroclor 1254) affects the hydrophilic membrane-bound ion dependent ATPases of the liver cells … …American Journal of Pharmacology and Toxicology, Vol. 8, Issue 2 · 13 Jul 2013 · 6,791 accessesView PDF Cite

Research ArticleOpen accessIn the abstractMicrowave Assisted Bioethanol Production from Sago Starch by Co-Culturing of Ragi Tapai and Saccharomyces CerevisiaeN. Saifuddin, Refal HussainProblem statement: Environmental issues such as global warming and recent events throughout the world, including the shortage of petroleum crude oil, the sharp increase in the cost of oil and the political instability of some crude oil producing countries, have demonstrated the vulnerability of the present sources for liquid fuel. These situations have created great demand for ethanol from fermentation … …Journal of Mathematics and Statistics, Vol. 7, Issue 3 · 18 Jul 2011 · 7,898 accessesView PDF Cite

Research ArticleOpen accessIn the abstractOligoglucans as Elicitors of an Enzymatic Antioxidant System in Zucchini Squash (Cucurbita pepo L.) Seedlings at Low TemperatureAbel Ceron-Garcia, Gustavo Adolfo Gonzalez-Aguilar, Irasema Vargas-Arispuro et al.Abstract: Problem statement: Non-controlled low temperatures affect physiological and biochemical processes leading to Reactive Oxygen Species (ROS) accumulation in plants, which normally are stabilized by the antioxidant system. Superoxide Dismutase (SOD), Ascorbate Peroxidase (APX) and Catalase (CAT) are the most important ROS-detoxification enzymes in stressed plants. Approach: The activity of these enzymes were examined in response to fungal Oligoglucans (OG) … …American Journal of Agricultural and Biological Sciences, Vol. 6, Issue 1 · 15 Dec 2010 · 8,306 accessesView PDF Cite

Research ArticleOpen accessIn the abstractUse of a Mixture of Thermophilic Enzymes Produced by the Fungus Thermoascus aurantiacus to Enhance the Enzymatic Hydrolysis of the Sugarcane Bagasse CelluloseJ. R. Monte, W. Carvalho, A. M. F. MilagresProblem statement: The production of hydrolytic enzymes by T. aurantiacus has been performed under solid-state fermentations using lignocellulosic materials. The influences of the inoculum size and of the fermentation medium on the production of hemicellulases and cellulases were studied. Filtrates from the cultures were used to hydrolyze a pulp of sugarcane bagasse and the produced enzymes were shown to be … …American Journal of Agricultural and Biological Sciences, Vol. 5, Issue 4 · 13 Dec 2010 · 9,212 accessesView PDF Cite

Research ArticleOpen accessIn the abstractGlomalin Production and Microbial Activity in Soils Impacted by Gypsum Mining in a Brazilian Semiarid AreaAdalia Cavalcanti do Espírito Santo Mergulhao, Helio Almeida Burity, Fabio Sérgio Barbosa da Silva et al.Problem statement: Mining activities involve the removal of the vegetal cover and the soil organic layer, causing a severe environmental impact. In Northeast Brazil, 40% of the world’s crude gypsum is found in a semiarid area, making this region responsible for 95% of the gypsum demand in the national market. Although economically important, this activity is harmful to the environment. … …American Journal of Agricultural and Biological Sciences, Vol. 5, Issue 4 · 11 Nov 2010 · 9,023 accessesView PDF Cite

Research ArticleOpen accessIn the abstractExpression, Purification and Activity Assay of the Recombinant Protein of Catechol-O-Methyltransferase from Chinese White Shrimp (Fenneropenaeus chinensis)Dian-Xiang Li, Xin-Jun Du, Xiao-Fan Zhao, Jin-Xing WangProblem statement: We have previously cloned a gene of Chinese white shrimp Catechol O-Methyltransferase (designated Fc-COMT) and characterized the gene expression pattern. In this study, expression and purification as well as activity assay of the recombinant Fc-COMT was further conducted. Approach: Using pET-30a (+) as a prokaryotic expression vector, the recombinant Fc- COMT was expressed in the supernatant of Escherichia … …American Journal of Biochemistry and Biotechnology, Vol. 6, Issue 3 · 30 Sep 2010 · 7,770 accessesView PDF Cite

Research ArticleOpen accessIn the abstractFish Spoilage Mechanisms and Preservation Techniques: ReviewA. E. Ghaly, D. Dave, S. Budge, M. S. BrooksProblem statement: Spoilage of food products is due to chemical, enzymatic or microbial activities One-fourth of the world’s food supply and 30% of landed fish are lost through microbial activity alone. With the ever growing world population and the need to store and transport the food from one place to another where it is needed, food preservation becomes necessary in … …American Journal of Applied Sciences, Vol. 7, Issue 7 · 31 Jul 2010 · 59,795 accessesView PDF Cite

Research ArticleOpen accessIn the abstractGenomic Distance between Thymidylate Synthase and Dihydrofolate Reductase Genes Does Not Correlate With Phylogenetic Evolution in BacteriaVitor Hugo MoreauProblem statement: Dihydrofolate Reductase (DHFR) and Thymidylate Synthase (TS) exist as bifunctional enzymes coded into unique polypeptide chain in protozoans. Bifunctional DHFR-TS is associated with an increase in the enzymatic activity by channeling the substrate between the active sites. In some bacteria, DHFR and TS genes are neighbors in the genome, whereas in others, they are located millions of base … …American Journal of Biochemistry and Biotechnology, Vol. 6, Issue 1 · 31 Mar 2010 · 8,188 accessesView PDF Cite