40 results for “Tissue culture”

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Research ArticleOpen accessIn the abstractApplication of a Serum/Protein-Free Medium for the Growth of Mammalian Cell Lines and High Level Production of Classical, Variant and Very Virulent Strain of Infectious Bursal Disease Virus (IBDV)Ane Franciele Tapparo, Paloma Oliveira Tonietti, Maria CecÍlia R. Luvizotto, Tereza C. CardosoThe aim of this study was to verify the capacity of IBDV strains, Lukert, F52/70 and very virulent Brazilian genotype (G11) to infect and replicate on cell culture maintained without serum or protein supplementation. BHK21 and CER cells were directed adapted to growth under absence of protein and/or fetal bovine serum with addition of 5 µg mL-1 of insulin-like growth … …American Journal of Agricultural and Biological Sciences, Vol. 3, Issue 2 · 30 Jun 2008 · 7,656 accessesView PDF Cite

Research ArticleOpen accessIn the abstractSurface Properties of Cell-treated Polyethylene TerephthalateBing Shi, Hong Liang, Thomas B. Kuhn, Lawrence K. DuffyThe materials used in artificial joints undergo degradation through fatigue and corrosive wear in human body. The lifetime for well-designed artificial joints like hip joints is at most 12 years and a patient will usually have two total joint replacements during his/her lifetime. Tissue engineering, an alternative to total joint implantation, is the replacement of damaged tissue with the tissue … …American Journal of Biochemistry and Biotechnology, Vol. 2, Issue 4 · 31 Dec 2006 · 6,704 accessesView PDF Cite

Research ArticleOpen accessIn the abstractInduced Microcracking Affects Osteoblast Attachment on Hydroxyapatite Scaffolds for Tissue EngineeringIan O. Smith, Melissa J. Baumann, Eldon D. CaseBone microdamage caused by routine activity plays an important role in triggering targeted and nontargeted bone remodeling. Targeted remodeling occurs near localized areas of microdamage[1-4]. We hypothesize that bone remodeling may be directly and positively influenced by inducing microcracks in hydroxyapatite (HA) scaffolds for bone tissue engineering. A study by Case et al. showed microcracking in HA discs having >98% … …American Journal of Biochemistry and Biotechnology, Vol. 2, Issue 3 · 30 Sep 2006 · 6,557 accessesView PDF Cite

Case ReportOpen accessIn the abstractOsteomyelitis Caused by Mycobacterium fortuitum-a Case ReportClarissa Jane Lyngdoh, Anne Khyriem, W. Valarie Lyngdoh, Tashi KhonglahNon-tuberculous rapidly growing mycobacteria have been known to cause a variety of skin and soft tissue infection. The diagnosis of NTM in bone and joint infections is easily missed because of non-specific signs and symptoms. We report a case of a 65 year old male with osteomyelitis of the right knee of 4 years duration. The patient had no prior … …American Journal of Infectious Diseases, Vol. 12, Issue 1 · 18 Feb 2016 · 7,263 accessesView PDF Cite

Research ArticleOpen accessIn the abstractEnhancement of in Vitro Rooting By Gelling Agents and Activated Charcoal in Rehmannia Glutinosa L.Aye Aye Thwe, Hyun Ho Kim, Haeng Hoon Kim, Sang Un ParkPlant tissue culture has proven to be commercially important for the replication of various plants species, including medicinal and ornamental plants. This technique has been used extensively in research for applications in agriculture and forestry. This study examines the effect of activated charcoal and the relative effectiveness of Gelrite compared to Phytagar. The number of roots per explant (8.2) and … …OnLine Journal of Biological Sciences, Vol. 15, Issue 2 · 12 Jun 2015 · 7,103 accessesView PDF Cite

Research ArticleOpen accessIn the abstractMembrane Binding Activity with Virus in White Spot Syndrome Virus-Infected Fenneropenaeus chinensisLiu Qing-hui, Huang Jie, Chen Ting, Yang BingProblem statement: White Spot Syndrome Virus (WSSV) is one of the most disastrous pathogens in shrimp culture, having caused high mortality in many cultured shrimp species. It is well known that the most important step of viral infection is cell attachment. Approach:Therefore determining the binding activity of shrimp cells to WSSV is an important to evaluate anti-infection ability and understanding … …American Journal of Agricultural and Biological Sciences, Vol. 4, Issue 2 · 30 Jun 2009 · 7,496 accessesView PDF Cite

Research ArticleOpen accessIn the abstractExpression of Exosome-Derived MicroRNAs miR-21 and miR-135 are Differentially Regulated Among Dental Pulp Stem CellsMatthew Hunsaker, Brandon Richards, Adelle Fuller et al.Dental Pulp Stem Cells (DPSCs) are a type of mesenchymal stem cell that has the potential to differentiate into various types of cells and tissue. Previous research has demonstrated that DPSCs can be easily accessed and isolated from both permanent and deciduous teeth, such as wisdom teeth or third molars, although there is less information available about the mechanisms and … …Current Research in Medicine, Vol. 11, Issue 1 · 10 Jan 2023 · 10,533 accessesView PDF Cite

Research ArticleOpen accessIn the abstractResponse of Different Agrobacterium Rhizogenes Strains for in vitro Hairy Root Induction and Accumulation of Rosmarinic Acid Production in Agastache RugosaWoo Tae Park, Thanislas Bastin Baskar, Sun Kyung Yeo et al.In this study a total of seven different Agrobacterium rhizogenes strains were evaluated for their ability to transform the plant Agastache rugosa and to produce the secondary metabolite rosmarinic acid. All the strains of A. rhizogenes i.e., 13333, 15834, A4, LBA9402, R1000, R1200 and R1601 strains tested here in this study, were able to induce hairy root formation in leaf … …OnLine Journal of Biological Sciences, Vol. 17, Issue 3 · 3 Sep 2017 · 7,866 accessesView PDF Cite

Research ArticleOpen accessIn the abstractPassage Effect on Aging of Human Umbilical Cord Derived Mesenchymal Stem CellDian Mediana, Isabella Kurnia Liem, Jeanne Adiwinata Pawitan, Noviyanti GoeiTo be used in regenerative medicine, cells should be checked for various conditions, including cell aging. This study aimed to learn senescent profile and its relation to cell viability, proliferation and cell size in various passages, which were done in α-MEM-10% PRP medium, until senescence-associated β-galactosidase (SA-β-Gal) positive cells were found. Stem cells were isolated from umbilical cord tissue by … …OnLine Journal of Biological Sciences, Vol. 15, Issue 3 · 31 Jul 2015 · 8,039 accessesView PDF Cite

Research ArticleOpen accessAll words, across the recordKhaen: An Application of Isan Local Wisdom for Conservation Revitalization and Development of Raw Materials and Khaen Making for Value-Added CultureChampadaeng Sithisak, Bunsom Yodmalee, Khaentaphon SamdaengdejProblem statement: The electricity in recent time, the raw materials for making “Khaen” (Lao reed mouthorgan) were decreased. The Reed Mouthorgan manufacturing was a high wisdom heritance. The objective of this research was to study the following issues: (1) the history of Reed Mouthorgan musical instrument, raw material and Reed Mouthorgan manufacturing in Esan Region. (2) The state of problems … …Journal of Social Sciences, Vol. 5, Issue 4 · 31 Dec 2009 · 7,686 accessesView PDF Cite