Membrane Binding Activity with Virus in White Spot Syndrome Virus-Infected Fenneropenaeus chinensis

Problem statement: White Spot Syndrome Virus (WSSV) is one of the most disastrous pathogens in shrimp culture, having caused high mortality in many cultured shrimp species. It is well known that the most important step of viral infection is cell attachment. Approach:Therefore determining the binding activity of shrimp cells to WSSV is an important to evaluate anti-infection ability and understanding the procedure of a viral infection. So this study aims to analyze binding activity of cell membrane with WSSV in shrimp of Fenneropenaeus chinensis (F. chienesis). WSSV was used to stimulate F. chinensis and binding activity in gills, muscle and hepatopancreas with virus within the procession of WSSV was measured. Results:The results showed that binding activity in gills with WSSV within infection stage (0-96 h) had no significant change. The highest binding activity was attained after infection 24-48 h in muscle. The binding activity in hepatopancreas with WSSV was lower during infection stage (0-96 h). Conclusion/Recommendation: The higher binding activity of WSSV to gills and muscle indicated that gills and muscle are the importance target tissue for WSSV infection. This suggested that receptor of WSSV existed in gills and muscle.