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Research ArticleOpen accessIn the author namesEvaluation of Japanese Quail Egg-Yolk Extender in Cryopreservation of Cockerel SemenRantloko Rolly Maapola, Khathutshelo Agree Nephawe, Tshimangadzo Lucky Nedambale et al.The purpose of this study was to examine the effects of quail egg yolk extenders in the cryopreservation of cockerel semen. Ten Venda cockerels were chosen randomly and semen was collected twice a week with a two-day rest period using an abdominal massage technique. Immediately following semen collection, semen samples were evaluated for microscopic parameters (sperm cell motility, viability, morphology, … …American Journal of Animal and Veterinary Sciences, Vol. 18, Issue 1 · 25 Mar 2023 · 5,638 accessesView PDFCite
Research ArticleOpen accessIn the author namesStudy the Efficacy of Different Concentrations of Coconut Water on Boar Semen Following Equilibration at 18°C for Different HoursMduduzi M. Tshabalala, Masindi L. Mphaphathi, Thivhilaheli R. Netshirovha et al.A rich natural resourcesuch as Coconut Water (CCW) features high concentrations of antioxidants,nutrients, amino acids, sugars, and electrolytes. These herbal extractinfusions are used in semen extenders to preserve animal sperm. In addition,CCW is important for in vitro productionof embryos. The purpose of this study was to investigate the use of coconutwater as an alternative bulking agent for boar semen after … …American Journal of Animal and Veterinary Sciences, Vol. 17, Issue 4 · 23 Nov 2022 · 7,040 accessesView PDFCite
Research ArticleOpen accessIn the author namesEffect of two Different Permitting and Combination of Cryoprotectants on Cattle Oocytes Maturation Rate Following Brilliant Cresyl Blue ExposureMasindi Lottus Mphaphathi, Hester Adri O'Neill, Tshimangadzo Lucky NedambaleThe objectives of this study were to elucidate the toxicity of Dimethyl sulfoxide (DMSO), Ethylene glycol (EG), and the combination of both permitting cryoprotectants (CPAs) to cattle oocytes and the effectiveness of Brilliant cresyl blue (BCB) on immature oocytes pre-selection. Cattle ovaries were collected from the local slaughterhouse and transported within 2 hours of slaughter. The oocytes were exposed to … …American Journal of Animal and Veterinary Sciences, Vol. 17, Issue 2 · 6 May 2022 · 6,201 accessesView PDFCite
Research ArticleOpen accessIn the author namesEffects of Bradyrhizobium Japonicum on Nitrogen Concentration in Soybean Leaves and Seeds Cultivated on Acidic SoilsBen Tshibuyi Kasu-Bandi, Fabien Kitengie Kitengie, Jonas Lwamuguma Bagaluza et al.The legume-rhizobium symbiosis plays an important role in Nitrogen (N) uptake by plants, more particularly in the humid tropical region where soils are deeply weathered and have poor electrochemical properties. However, the influence of Bradyrhizobium on N allocation in soybean organs is not clearly understood. Here, we assessed the effect of Bradyrhizobium japonicum on the N concentration of soybean leaves … …American Journal of Agricultural and Biological Sciences, Vol. 17, Issue 1 · 24 Jan 2022 · 7,663 accessesView PDFCite
Research ArticleOpen accessIn the author namesA Comparative Study on the Reproductive Performance of South African Indigenous Sheep Breeds Following Oestrus SynchronizationJabulani Nkululeko Ngcobo, Tshimangadzo Lucky Nedambale, Tlou Caswell Chokoe, Fhulufhelo Vincent RamukhithiThe aim of the study was to compare the effect of age and live body weight on the oestrus response, duration, pregnancy rate and lambing rate among South African indigenous sheep breeds (Zulu sheep = 36, Bapedi sheep = 26, Damara sheep = 10 and Namaqua Afrikaner sheep = 9). Control Intravaginal Drug Release Dispensers (CIDR’s) were inserted into the … …American Journal of Animal and Veterinary Sciences, Vol. 17, Issue 1 · 14 Jan 2022 · 7,862 accessesView PDFCite
Research ArticleOpen accessIn the author namesSuperovulation Response and Embryo Quality Recovered Following Flushing Nguni Heifers and CowsAyanda Maqhashu, Masindi L. Mphaphathi, Pokgedi J Sebei et al.The aims of the study were to compare super ovulatory response, fertilization rate and evaluate embryo quality recovered from super ovulated Nguni stud cows and heifers. Nguni cows (n = 15) and heifers (n = 10) aged 4-6 and 2 years, respectively, were used as embryo donors. Embryo donors were synchronized for estrus before superovulation. For superovulation donors were administered … …American Journal of Animal and Veterinary Sciences, Vol. 16, Issue 4 · 25 Nov 2021 · 6,431 accessesView PDFCite
Research ArticleOpen accessIn the author namesStudy the Effect of Extracted Low-Density Lipoprotein from Egg Yolk on the Cryopreservation of Nguni Bull SemenMduduzi M. Tshabalala, Masindi Lottus Mphaphathi, Khathutshedzo A. Nephawe et al.The mechanism on how Egg Yolk (EY) helps sperm to survive the low temperature outside the animal body during cryopreservation and thawing process is not yet known. It is usually believed that the Low-Density Lipoprotein (LDL) is the major component that provides protection. The present study was undertaken to assess the cryo-protective effect of different concentrations (6, 8, 10 and … …American Journal of Animal and Veterinary Sciences, Vol. 16, Issue 3 · 13 Sep 2021 · 6,915 accessesView PDFCite
Research ArticleOpen accessIn the author namesEffect of Repeated Freezing and Thawing on Nguni Sperm Parameters Evaluated by Computer Assisted Sperm Analyzer SystemMasindi Lottus Mphaphathi, Tshimangadzo Lucky NedambaleThe aims of the current study were to assess the effect of double freezing and thawing on Nguni semen parameters. Semen was collected from Nguni bulls. The Sperm Class Analyzer® system was used to evaluate sperm motility rates prior and post freezing, repeatedly. Semen samples were diluted with egg yolk citrate extender. Cooled semen samples were loaded into straws and … …American Journal of Animal and Veterinary Sciences, Vol. 16, Issue 1 · 27 Jan 2021 · 6,591 accessesView PDFCite
Research ArticleOpen accessIn the author namesSeasonal Variations in Semen Parameters of Zulu Rams Preserved at 10°C for 72 H During Breeding and Non-Breeding SeasonJabulani Nkululeko Ngcobo, Khathutshelo Agree Nephawe, Ayanda Maqhashu, Tshimangadzo Lucky NedambaleThe study was conducted to evaluate the seasonal variations in semen parameters of Zulu rams preserved at 10°C for 72 h. The study site was the Agricultural Research Council (ARC) Irene, during the breeding season and the non-breeding season. Zulu rams (n = 6, age = 3 years and average weight = 35 kg ±2.29) were used in this study. … …American Journal of Animal and Veterinary Sciences, Vol. 15, Issue 3 · 28 Aug 2020 · 7,302 accessesView PDFCite
Research ArticleOpen accessIn the author namesPhenotypic Characterisation of South African Unimproved Indigenous and Tankwa GoatsFhulufhelo Vincent Ramukhithi, Khoboso Christina Lehloenya, Antoinette Kotze et al.The aim of this study was to characterise and compare phenotypic characteristics of South African unimproved indigenous and Tankwa goats. Phenotypic characteristics of 44 South African unimproved indigenous and 41 Tankwa goats were assessed. The qualitative characteristics were assessed through visual appraisal. Quantitative characteristics such as body measurements were obtained using a flexible tape, while body weight was determined by … …American Journal of Animal and Veterinary Sciences, Vol. 14, Issue 4 · 23 Oct 2019 · 8,156 accessesView PDFCite