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342 results for “In vitro”
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Research ArticleOpen accessEvery word in the titleAugmentation of Temperatures, Media and Cryoprotectants on in vitro Fertilization of Pig OocytesTumelo Thelma Maduwa, Masindi Lottus Mphaphathi et al.There are numerous challenges to the large-scale production of pig embryos using in vitro procedures. The aim of the present study was to compare the different holding temperatures, in vitro maturation media, and permeating cryoprotectants in the in vitro fertilization of pig oocytes. Ovaries were obtained from the local slaughterhouse, oocytes were retrieved by means of aspiration and slicing techniques … …American Journal of Animal and Veterinary Sciences, Vol. 19, Issue 3 · 29 Aug 2024 · 4,656 accessesView PDFCite
Research ArticleOpen accessEvery word in the titleTestosterone Concentration, Libido and Spermatozoa Quality in Gembrong Goats after in vivo and in vitro PGF2α AdministrationHusnurrizal, Sri Wahyuni et al.The Gembrong goat population is currently in a critical state. The preliminary study results show that one of the factors causing the population decline is the low fertility of male goats characterized by low sperm quality. The improvement of the spermatozoa quality can be performed with the administration of PGF2α. This study aims to determine the difference in semen quality … …American Journal of Animal and Veterinary Sciences, Vol. 19, Issue 2 · 29 Apr 2024 · 5,549 accessesView PDFCite
Research ArticleOpen accessEvery word in the titleIn Vitro Antimicrobial Activity of Modified Lysozyme (Lys 90) and Herbal Extracts Against Multidrug-Resistant Escherichia coliEni Kusumaningtyas, Bagem BR Sembiring, Andriani et al.The emergence of Antimicrobial Resistant (AMR) poses a significant challenge to the poultry farming industry. This study investigated the potential of Lysozyme (Lys), modified Lysozyme (Lys 90), an herbal extract, and their combination (herbs + Lys 90) as alternative antimicrobial agents against Multidrug-Resistant Escherichia coli (MDR-E. coli). Lys 90 was prepared by heating Lys at 90 °C for 20 minutes. … …OnLine Journal of Biological Sciences, Vol. 25, Issue 3 · 1 Dec 2025 · 2,079 accessesView PDFCite
Literature ReviewOpen accessEvery word in the titleControl of Contamination of Tissue Plant Cultures During in Vitro Clonal MicropropagationIrina Anikina, Nursultan Kaynidenov, Dora Dayu Rahma Turista et al.Plant micropropagation is currently an important method for modern large-scale propagation of valuable plant crops. Among the risks limiting the success of this method is the contamination of cultured plants. Conventional surface sterilization methods are not always effective against internal infection of plant tissues, and secondary contamination of tissue cultures during replication is also a major problem. This causes scientific … …OnLine Journal of Biological Sciences, Vol. 25, Issue 2 · 8 Apr 2025 · 8,714 accessesView PDFCite
Research ArticleOpen accessEvery word in the titleEffect of Temperature Change and Estradiol Levels on Cattle Oocytes Developmental Competency: Comparing X-Sexed and Non-Sexed Sperm Following in vitro FertilizationMaleke Dimpho Sebopela, Masindi Lottus Mphaphathi et al.The study aimed to investigate the effects of temperature change and estradiol (E2) hormone level on In Vitro Maturation (IVM) and fertilization of cattle oocytes using X-sexed and non-sexed sperm. In experiment 1: The oocytes were incubated at various temperatures of 35.5, 36.5, 37.5, 38.5, 39.5 and 40.5°C under similar conditions with 5% CO2, in humidified air for 22 h. … …American Journal of Animal and Veterinary Sciences, Vol. 18, Issue 4 · 16 Nov 2023 · 5,324 accessesView PDFCite
Research ArticleOpen accessEvery word in the titleIn vitro Ruminal Cumulative Gas and Methane Production, Enzyme Activity, Fermentation Profile and Nutrient Digestibility on Feed Supplemented with Organic SeleniumMoh. Sofi’ul Anam, Andriyani Astuti, Budi Prasetyo Widyobroto et al.Methane (CH4) emissions are indeed a global environmental concern and ruminants are a significant source of these emissions; however, there is limited understanding regarding the contribution of organic Selenium (Se) in mitigating CH4 emissions. Hence, this study investigated the impact of supplementing organic Se from Selenomethionine (Se-Met) on the in vitro ruminal cumulative gas and CH4 production, fermentation profile, enzyme … …American Journal of Animal and Veterinary Sciences, Vol. 18, Issue 4 · 26 Oct 2023 · 5,222 accessesView PDFCite
Research ArticleOpen accessEvery word in the titleComparison of in vitro Maturation Media on Cattle Oocytes after in vitro Embryo ProductionSindisiwe Mbali Sithole, Masindi Lottus Mphaphathi et al.The study was conducted to compare differences in vitro maturation media and their effect on subsequent in vitro embryo production. The study procedures were approved by the agricultural research council animal ethics committee (Ref no: APAEC 2020/05) and the Tshwane university of technology animal research ethics committee (Ref no: AREC 2021/08/004). Follicular fluid containing a suspension of oocytes was retrieved … …American Journal of Animal and Veterinary Sciences, Vol. 18, Issue 1 · 23 Feb 2023 · 6,919 accessesView PDFCite
Research ArticleOpen accessEvery word in the titleNon-Antibiotic Immunomodulatory Combination Treatment for Staphylococcus aureus Infections: In vitro Preliminary Studies IJatna Isha Rivas Zarete, Dominique Lyles, Benjamin Adu-AddaiDespite advancements in prevention measures, infectious mastitis is a common disease of economic significance in cattle. Clinical mastitis affects 20 to 25% of cows each year. Staphylococcus aureus (S. aureus) causes an estimated 10-11.7% of clinical bovine infectious mastitis cases in the US. Antibiotic therapy tends to fail 50% of the time and nature and vaccine immunity usually fails, due … …American Journal of Infectious Diseases, Vol. 18, Issue 4 · 14 Feb 2023 · 5,680 accessesView PDFCite
Research ArticleOpen accessEvery word in the titleIn vitro Kinematics Semen Tolerance to Oxidative StressCyril Mpho PilaneInterpretation of semen kinematic parameters remain as a point of discussion in predicting semen quality following processes like cryopreservation. During cryopreservation, oxidative stress remains a challenge in promoting post-thaw semen quality. Comparison of livestock semen exposed to oxidative stress, in vitro, highlight various aspects of sperm cell movement, like kinematics, to predict their qualities. Sperm cell kinematics includes the measurement … …American Journal of Animal and Veterinary Sciences, Vol. 19, Issue 3 · 13 Sep 2024 · 4,565 accessesView PDFCite
Research ArticleOpen accessEvery word in the titleOptimization of Preparation Procedures of Phlorizin-Zn (Ⅱ) Complex and Its Antioxidant Activity Study in vitroYuqing Zhang, Yaping Li, Guorui Yang et al.Flavonoid metal complexes possess more effective antioxidant properties than flavonoids. However, the preparation of the phlorizin-Zn (Ⅱ) complex and its antioxidant activity has not been reported. In this research, the preparation procedures of the phlorizin-Zn (Ⅱ) complex were enhanced using by single factor test and Response Surface Methodology (RSM). The optimal preparation procedures of the phlorizin-Zn (II) complex were as … …American Journal of Biochemistry and Biotechnology, Vol. 19, Issue 4 · 3 Apr 2024 · 4,761 accessesView PDFCite