37 results for “High-performance liquid chromatography”

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Research ArticleOpen accessIn the abstractEffect of Plant Growth Regulators on Callus, Cell Suspension and Cell Line Selection for Flavonoid Production from Pegaga (centella asiatica L. urban)Suat Hian Tan, Radzali Musa, Arbakariya Ariff, Mahmood MaziahProblem statement: Considering pegaga medicinal properties and over-exploitation, the requirement for a tissue culture technique as an alternative production system was crucial. Approach: Investigation of cell suspension culture response to different plant growth regulators (PRGs) for flavonoid production from elite cell line was carried out. Callus cultures were initiated from the leaf explants of Centella asiatica on Murashige and Skoog … …American Journal of Biochemistry and Biotechnology, Vol. 6, Issue 4 · 31 Dec 2010 · 11,254 accessesView PDF Cite

Research ArticleOpen accessIn the abstractExpression, Purification and Activity Assay of the Recombinant Protein of Catechol-O-Methyltransferase from Chinese White Shrimp (Fenneropenaeus chinensis)Dian-Xiang Li, Xin-Jun Du, Xiao-Fan Zhao, Jin-Xing WangProblem statement: We have previously cloned a gene of Chinese white shrimp Catechol O-Methyltransferase (designated Fc-COMT) and characterized the gene expression pattern. In this study, expression and purification as well as activity assay of the recombinant Fc-COMT was further conducted. Approach: Using pET-30a (+) as a prokaryotic expression vector, the recombinant Fc- COMT was expressed in the supernatant of Escherichia … …American Journal of Biochemistry and Biotechnology, Vol. 6, Issue 3 · 30 Sep 2010 · 7,770 accessesView PDF Cite

Research ArticleOpen accessIn the abstractCross Reactivity between Dromedary Whey Proteins and IgG Anti Bovine α-Lactalbumin and Anti Bovine β-LactoglobulinN. Youcef, D. Saidi, F. Mezemaze et al.Problem statement: Our aim was to enhance the data on antigenic properties of dromedary whey proteins. Approach: The identification of the whey proteins was carried by SDS-page and Reversed phase high performance liquid chromatography (RP-HPLC). The cross-reactivity of dromedary whey proteins with IgG anti bovine β-lactoglobulin and anti bovine α-lactalbumin, obtained by immunisation of Balb/c mice, was carried out by … …American Journal of Applied Sciences, Vol. 6, Issue 8 · 31 Aug 2009 · 7,429 accessesView PDF Cite

Research ArticleOpen accessIn the abstractMass Spectrometry/Mass Spectrometry Study on the Degradation of B-Aflatoxins in Maize with Aqueous Citric AcidA. Méndez-Albores, I. Nicolás-Vázquez, R. Miranda-Ruvalcaba, E. Moreno-MartÍnezDegradation of B-aflatoxins in maize by means of 1N aqueous citric acid was confirmed by the AFLATEST immunoaffinity column method, High Performance Liquid Chromatography (HPLC), tandem mass spectrometry (MS/MS) and computational information. The AFLATEST and HPLC assays showed that 96.7% degradation occurred in maize contaminated with 93 ng g-1 when treated with the aqueous citric acid. Two major products, produced … …American Journal of Agricultural and Biological Sciences, Vol. 3, Issue 2 · 30 Jun 2008 · 8,049 accessesView PDF Cite

Research ArticleOpen accessIn the abstractSeparation of Guanine and Hypoxanthine with Some Ionic Liquids in RP-HPLCJ. Zheng, K. H. RowIn this paper, guanine and hypoxanthine were separated with four different ionic liquids as additives for the mobile phase using reversed phase high performance liquid chromatography (RP-HPLC). The ionic liquids, 1-butyl-3-methylimidazolium tetrafluoroborate ([BMIm][BF4]), 1-ethyl-3-methylimidazolium tetrafluoroborate ([EMIm][BF4]), 1-ethyl-3-methylimidazolium methylsulfate ([EMIm][MS]) and 1-octyl-3-methylimidazolium methylsulfate ([OMIm][MS]) were used. Guanine and hypoxanthine couldn’t be separated with many different kinds of unadjusted mobile phase, such … …American Journal of Applied Sciences, Vol. 3, Issue 12 · 31 Dec 2006 · 6,892 accessesView PDF Cite

Research ArticleOpen accessIn the abstractThe Effects of Chinese Traditional Processing Method on Components in Semen Sinapis AlbaeLifang Liu, Hui Zhou, Suqin Sun et al.Techniques of infrared (IR) spectrophotometry, High Performance Liquid Chromatography (HPLC) and HPLC-mass spectrometry are employed in this study to reveal the changes of the components in Semen sinapis Albae during the Chinese traditional processing. While the degradation of proteins and polysaccarides can be observed, sinapine in this drug can be known to decompose immediately after 15 mins during processing. A … …American Journal of Biochemistry and Biotechnology, Vol. 1, Issue 2 · 30 Jun 2005 · 6,810 accessesView PDF Cite

Research ArticleOpen accessAll words, across the recordDevelopment and Validation of High Performance Liquid Chromatographic Method for the Simultaneous Determination of Ceftazidime and Sulbactam in Spiked Plasma and Combined Dosage form-ZydotamMasoom Raza Siddiqui, Abu Tariq, Manu Chaudhary et al.Problem statement: To develop a sensitive method to determine simultaneously ceftizidime and sulbactam in spiked plasma and combined formulation. Approach: In this study an isocratic High performance liquid chromatographic method with UV detection at 230 nm was described for simultaneous determination of Ceftazidime and sulbactam sodium in plasma and combined dosage form. Chromatographic separation of two drugs was achieved on … …American Journal of Applied Sciences, Vol. 6, Issue 10 · 31 Oct 2009 · 7,672 accessesView PDF Cite